Biomaterials in Tooth Tissue Engineering

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

Journal of Dental Research ◽

10.1177/154405910408300703 ◽

2004 ◽

Vol 83 (7) ◽

pp. 523-528 ◽

Cited By ~ 259

Author(s):

M.T. Duailibi ◽

S.E. Duailibi ◽

C.S. Young ◽

J.D. Bartlett ◽

J.P. Vacanti ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Engineering Applications ◽

Adult Rat ◽

Dental Tissues ◽

Biodegradable Scaffolds ◽

Bioengineered Tooth ◽

Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

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Tooth Tissue Engineering and Regeneration—a Translational Vision!

Journal of Dental Research ◽

10.1177/154405910408300701 ◽

2004 ◽

Vol 83 (7) ◽

pp. 517-517 ◽

Cited By ~ 22

Author(s):

Anthony J. (Tony) Smith

Keyword(s):

Tissue Engineering ◽

Tooth Tissue

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Evaluation of scaffold materials for tooth tissue engineering

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.32749 ◽

2010 ◽

Vol 9999A ◽

pp. NA-NA ◽

Cited By ~ 3

Author(s):

Takayuki Ohara ◽

Toshimitsu Itaya ◽

Kazutada Usami ◽

Yusuke Ando ◽

Hiroya Sakurai ◽

...

Keyword(s):

Tissue Engineering ◽

Scaffold Materials ◽

Tooth Tissue

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Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells

Tissue Engineering Part A ◽

10.1089/ten.tea.2014.0617 ◽

2015 ◽

Vol 21 (21-22) ◽

pp. 2639-2648 ◽

Cited By ~ 6

Author(s):

Ricardo Luiz Pisciolaro ◽

Monica Talarico Duailibi ◽

Neil Ferreira Novo ◽

Yara Juliano ◽

Debora Pallos ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Tissue Culture ◽

Culture Medium ◽

Tissue Culture Medium ◽

Blood Products ◽

Dental Stem Cells ◽

Tooth Tissue

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Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering

Biomaterials ◽

10.1016/j.biomaterials.2006.01.055 ◽

2006 ◽

Vol 27 (17) ◽

pp. 3238-3248 ◽

Cited By ~ 132

Author(s):

Y SUMITA ◽

M HONDA ◽

T OHARA ◽

S TSUCHIYA ◽

H SAGARA ◽

...

Keyword(s):

Tissue Engineering ◽

Collagen Sponge ◽

Tooth Tissue

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Tooth Tissue Engineering: Potential and Pitfalls

Journal of Biomimetics Biomaterials and Tissue Engineering ◽

10.4028/www.scientific.net/jbbte.12.59 ◽

2012 ◽

Vol 12 ◽

pp. 59-81 ◽

Cited By ~ 4

Author(s):

Sweta Gupta ◽

Chhavi Sharma ◽

Amit Kumar Dinda ◽

Amiya K. Ray ◽

Narayan C. Mishra

Keyword(s):

Tissue Engineering ◽

Tooth Loss ◽

Direct Pulp Capping ◽

Proper Selection ◽

Cell Scaffold ◽

Conventional Methods ◽

Specific Tissue ◽

Tooth Tissue ◽

Missing Tooth ◽

Selection Of

All over the world a large number of people suffer from tooth diseases like dental caries, tooth abscess, and plaques. Tooth loss or damage, which occurs frequently in our society are generally repaired by applying several conventional methods, such as root-canal treatment, direct pulp capping and dental implants. These methods are quite painful, create damage to the surrounding tooth tissues and also may at times have adverse side-effects. The limitations of the conventional methods can be overcome by applying the concept of tooth tissue engineering. Tooth tissue engineering is the application of biosciences and engineering to regenerate a biofunctional tooth, which can be used to replace the missing tooth or repair the damaged tooth. Tissue engineering involves three key elements - cell, scaffold and growth factors, which interact with each other to regenerate a specific tissue. The success of tissue engineering depends on the proper selection of these three key elements and understanding the interactions among them. To bring us close to the realization of a tissue-engineered tooth, immense progress is going on in understanding how tooth is first developed, and there is a good advancement in tooth regeneration. In this review, “tooth tissue engineering” will be discussed, along with the recent advancements and challenges in bring a biofunctional tooth from laboratory out into clinical use.

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