Bone Regeneration Using Duck’s Feet-Derived Collagen Scaffold as an Alternative Collagen Source

2020 ◽  
pp. 3-13
Author(s):  
Jeong Eun Song ◽  
Muthukumar Thangavelu ◽  
Joohee Choi ◽  
Hunhwi Cho ◽  
Byung Kwan Moon ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold

In vivo Evaluation of a Collagen Scaffold Preconditioned with Adipose-derived Mesenchymal Stem Cells Used for Bone Regeneration A histological study

2018 ◽  
Vol 55 (4) ◽  
pp. 691-695
Author(s):  
Tudor Sorin Pop ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Klara Brinzaniuc ◽  
Simona Gurzu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Histological Study ◽  
Collagen Scaffold ◽  
Bone Defects ◽  
Collagen Scaffolds ◽  
In Vivo Evaluation ◽  
Calvarial Bone ◽  
Bone Apposition

The use of collagen scaffolds and stem cells for obtaining a tissue-engineering complex has been an important concept in promoting repair and regeneration of the bone tissue. Such units represent important steps in the development of an ideal scaffold-cell complex that would sustain new bone apposition. The aim of our study was to perform a histologic evaluation of the healing of critical-sized bone defects, using a biologic collagen scaffold with adipose-derived mesenchymal stem cells, in comparison to negative controls created in the adjacent bone. We used 16 Wistar rats and according to the study design 2 calvarial bone defects were created in each animal, one was filled with collagen seeded with adipose-derived stem cells and the other one was considered negative control. During the following month, at weekly intervals, the animals were euthanized and the specimens from bone defects were histologically evaluated. The results showed that these scaffolds were highly biocompatible as only moderate inflammation no rejection reactions were observed. Furthermore, the first signs of osseous healing appeared after two weeks accompanied by angiogenesis. Collagen scaffolds seeded with adipose-derived mesenchymal stem cells can be considered a promising treatment option in bone regeneration of large defects.

Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold

2009 ◽  
Vol 15 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Tadashi Kawai ◽  
Takahisa Anada ◽  
Yoshitomo Honda ◽  
Shinji Kamakura ◽  
Keiko Matsui ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Octacalcium Phosphate

Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration

Biomaterials ◽  
2017 ◽  
Vol 113 ◽  
pp. 203-216 ◽  
Author(s):  
Jin-long Sun ◽  
Kai Jiao ◽  
Li-na Niu ◽  
Yang Jiao ◽  
Qun Song ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Cell Homing

Polyphenols and recombinant protein activated collagen scaffold enhance angiogenesis and bone regeneration in rat critical-sized mandible defect

Cytotherapy ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. e8-e9
Author(s):  
G. Bhattarai ◽  
S. Poudel ◽  
M. Kim ◽  
H. Sim ◽  
H. So ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Mandible Defect

Bone regeneration utilizing a newly developed octacalcium phosphate/weakly denatured collagen scaffold

2020 ◽  
pp. 039139882092404
Author(s):  
Mayumi Kaneko ◽  
Keiji Shigeno ◽  
Mariko Wakatsuki ◽  
Akira Nakada ◽  
Yuji Inada ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tumor Resection ◽  
Collagen Scaffold ◽  
Collagen Matrix ◽  
Octacalcium Phosphate ◽  
Canine Model ◽  
Histopathological Analysis ◽  
Collagen Scaffolds ◽  
Skull Defects ◽  
Freeze Dried

Introduction: Autologous bone grafting or various bone-regenerating materials are used to treat bone defects caused by tumor resection and accident trauma. Octacalcium phosphate, a reasonable bone regenerative material, activates osteoblasts. We fabricated a composite material, octacalcium phosphate/weakly denatured collagen, as a new scaffold. We aimed to investigate the osteoregenerative effect of the octacalcium phosphate/weakly denatured collagen scaffold (compared with that of weakly denatured collagen) in skull defects in a canine model. Methods: Atelocollagen was extracted from porcine skin via pepsin treatment. The weakly denatured collagen scaffold was fabricated with a freeze-dried and thermally crosslinked atelocollagen suspension at pH 7.4. Octacalcium phosphate was synthesized using Ca-acetate and NaH2PO4. Octacalcium phosphate particles (diameter, 199–298 µm) were mixed with a collagen matrix to fabricate an octacalcium phosphate/weakly denatured collagen scaffold. Bilateral defects (diameter, 10 mm; full-thickness) were induced in dog skulls, and the octacalcium phosphate/weakly denatured collagen and weakly denatured collagen scaffolds were implanted into the defects. Results: Eight weeks after implantations, bone regeneration was evaluated via histopathological analysis. It revealed osteoblast infiltration and osteoregeneration in all defects treated with the octacalcium phosphate/weakly denatured collagen scaffold. The defects treated with weakly denatured collagen scaffold or without any scaffold mostly contained connective tissue, with no neo-osteogenesis. Discussion: The novel octacalcium phosphate/weakly denatured collagen scaffold better promotes osteoregeneration than the weakly denatured collagen scaffold; this “in situ tissue engineering” approach is potentially clinically applicable for bone reconstruction.

scholarly journals Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

2021 ◽  
Author(s):  
Yu-Xuan Ma ◽  
Kai Jiao ◽  
Qian-Qian Wan ◽  
Jing Li ◽  
Ming-Yi Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Sensory Nerves ◽  
Semaphorin 3A

Intrafibrillar silicified collagen scaffold promotes in-situ bone regeneration by activating the monocyte p38 signaling pathway

2018 ◽  
Vol 67 ◽  
pp. 354-365 ◽  
Author(s):  
Jin-long Sun ◽  
Kai Jiao ◽  
Qun Song ◽  
Chu-fan Ma ◽  
Chao Ma ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Signaling Pathway ◽  
Collagen Scaffold
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