scholarly journals Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

2021 ◽  
Author(s):  
Yu-Xuan Ma ◽  
Kai Jiao ◽  
Qian-Qian Wan ◽  
Jing Li ◽  
Ming-Yi Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Sensory Nerves ◽  
Semaphorin 3A

Intrafibrillar silicified collagen scaffold promotes in-situ bone regeneration by activating the monocyte p38 signaling pathway

2018 ◽  
Vol 67 ◽  
pp. 354-365 ◽  
Author(s):  
Jin-long Sun ◽  
Kai Jiao ◽  
Qun Song ◽  
Chu-fan Ma ◽  
Chao Ma ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Signaling Pathway ◽  
Collagen Scaffold

In vivo Evaluation of a Collagen Scaffold Preconditioned with Adipose-derived Mesenchymal Stem Cells Used for Bone Regeneration A histological study

2018 ◽  
Vol 55 (4) ◽  
pp. 691-695
Author(s):  
Tudor Sorin Pop ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Klara Brinzaniuc ◽  
Simona Gurzu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Histological Study ◽  
Collagen Scaffold ◽  
Bone Defects ◽  
Collagen Scaffolds ◽  
In Vivo Evaluation ◽  
Calvarial Bone ◽  
Bone Apposition

The use of collagen scaffolds and stem cells for obtaining a tissue-engineering complex has been an important concept in promoting repair and regeneration of the bone tissue. Such units represent important steps in the development of an ideal scaffold-cell complex that would sustain new bone apposition. The aim of our study was to perform a histologic evaluation of the healing of critical-sized bone defects, using a biologic collagen scaffold with adipose-derived mesenchymal stem cells, in comparison to negative controls created in the adjacent bone. We used 16 Wistar rats and according to the study design 2 calvarial bone defects were created in each animal, one was filled with collagen seeded with adipose-derived stem cells and the other one was considered negative control. During the following month, at weekly intervals, the animals were euthanized and the specimens from bone defects were histologically evaluated. The results showed that these scaffolds were highly biocompatible as only moderate inflammation no rejection reactions were observed. Furthermore, the first signs of osseous healing appeared after two weeks accompanied by angiogenesis. Collagen scaffolds seeded with adipose-derived mesenchymal stem cells can be considered a promising treatment option in bone regeneration of large defects.

scholarly journals Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

2020 ◽  
Author(s):  
Isabel Pereira ◽  
José Eduardo Pereira ◽  
Luís Maltez ◽  
Alexandra Rodrigues ◽  
Catarina Rodrigues ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Substitute ◽  
Bone Substitutes ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Micro Computed Tomography ◽  
Synthetic Bone Substitute ◽  
In Situ Forming ◽  
Bioactive Agents ◽  
In Situ Forming Hydrogels

Abstract The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike®) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike® granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.

Digital light processing strength-strong ultra-thin bioceramic scaffolds for challengeable orbital bone regeneration and repair in Situ

2021 ◽  
Vol 22 ◽  
pp. 100889
Author(s):  
Jingyi Wang ◽  
Xizhe Dai ◽  
Yiyu Peng ◽  
Mengtao Liu ◽  
Fengling Lu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Digital Light Processing

Bioabsorbable scaffold for in situ bone regeneration

2006 ◽  
Vol 60 (8) ◽  
pp. 386-392 ◽  
Author(s):  
R. Giardino ◽  
N Nicoli Aldini ◽  
M. Fini ◽  
M.C. Tanzi ◽  
S. Faré ◽  
...  
Keyword(s):  
Bone Regeneration

scholarly journals Composite Sponges for in Situ Alveolar Bone Regeneration Following Tooth Extraction

2017 ◽  
Vol 131 (3) ◽  
pp. 580-584 ◽  
Author(s):  
Z.A. Guclu ◽  
A.P. Hurt ◽  
L. Ohia ◽  
N.J. Coleman
Keyword(s):  
Bone Regeneration ◽  
Tooth Extraction ◽  
Alveolar Bone

Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold

2009 ◽  
Vol 15 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Tadashi Kawai ◽  
Takahisa Anada ◽  
Yoshitomo Honda ◽  
Shinji Kamakura ◽  
Keiko Matsui ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold ◽  
Octacalcium Phosphate

scholarly journals In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano-Hydroxyapatite/Collagen Scaffold

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Yan Chen ◽  
Zhi Huang ◽  
Xiaoming Li ◽  
Songjian Li ◽  
Zhilai Zhou ◽  
...  
Keyword(s):  
Collagen Scaffold ◽  
Reaction Cell ◽  
In Vitro Biocompatibility ◽  
In Situ Forming ◽  
Osteogenic Markers ◽  
Polymerase Chain ◽  
Cell Expression

The purpose of this study was to evaluate the in vitro cell biocompatibility of an in situ forming composite consisting of chitosan (CS), nano-hydroxyapatite and collagen (nHAC), which has a complex hierarchical structure similar to natural bone. MC3T3-E1 mouse calvarial preosteoblasts were cultured on the surface of the injectable CS/nHAC and CS scaffold. The proliferations of seeded MC3T3-E1 were investigated for 10 days. Cytotoxicity, cell proliferation, and cell expression of osteogenic markers such as alkaline phosphatase (ALP), type 1 collagen (COL-1), RUNX-2, and osteocalcin (OCN) were examined by biochemical assay and reverse transcription polymerase chain reaction. Cell viability and total cellularity (measured by dsDNA) were similar between the two scaffold groups. However, ALP, COL-1, OCN, and RUNX-2 production were significantly greater when osteoblasts were cultured on CS/nHAC scaffolds. The increase in osteogenic markers production on CS/nHAC scaffolds indicated that these scaffolds were superior to chitosan-only scaffolds in facilitating osteoblast mineralization. These results demonstrate the potential of the CS/nHAC scaffolds to be used in bone tissue engineering.

Bone Regeneration Using Duck’s Feet-Derived Collagen Scaffold as an Alternative Collagen Source

2020 ◽  
pp. 3-13
Author(s):  
Jeong Eun Song ◽  
Muthukumar Thangavelu ◽  
Joohee Choi ◽  
Hunhwi Cho ◽  
Byung Kwan Moon ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffold
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