Comparison of spatial distributions and food of allopatric populations of cutthroat trout (Salmo clarki) and Dolly Varden (Salvelinus malma) in two lakes and of sympatric populations in a third lake suggested interactive segregation. Allopatric cutthroat changed their depth distribution throughout the spring and summer but no diel migration was noted. Changes in food habits closely corresponded to seasonal changes in vertical distribution. A wide range of food organisms was eaten from surface, midwater, and benthic sources. Allopatric Dolly Varden underwent diel changes in spatial distribution, with many being caught in onshore waters near the surface at night. They were absent from such areas in the day, apparently occupying offshore waters at greater depths. Their diet throughout summer consisted largely of surface insects and zooplankton, with lesser amounts of bottom fauna.Sympatric cutthroat trout and Dolly Varden were spatially segregated throughout the summer. Cutthroat trout occurred and fed mostly near the surface in littoral areas. In contrast, Dolly Varden were offshore and benthic in distribution and fed primarily on bottom organisms. By comparing distribution and food habits in allopatric and sympatric populations, it was suggested that competition for food may contribute to their segregation in sympatry. Although segregation of these species when cohabiting may involve interactive processes, the possibility of selective differences being of importance cannot be dismissed.