Yield and biological nitrogen fixation of common bean (Phaseolus vulgaris L.) in Peru

1993 ◽  
Vol 152 (1) ◽  
pp. 87-91 ◽  
Author(s):  
A. Manrique ◽  
K. Manrique ◽  
J. Nakahodo
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Biological Nitrogen Fixation ◽  
Phaseolus Vulgaris L ◽  
Biological Nitrogen

scholarly journals Biological nitrogen fixation by irrigated dry bean (Phaseolus vulgaris L.) genotypes

2018 ◽  
Vol 98 (5) ◽  
pp. 1159-1167 ◽  
Author(s):  
Z. Akter ◽  
B.B. Pageni ◽  
N.Z. Lupwayi ◽  
P.M. Balasubramanian
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Biological Nitrogen Fixation ◽  
Rhizobium Leguminosarum ◽  
Phaseolus Vulgaris L ◽  
Dry Bean ◽  
Pinto Bean ◽  
Red Bean ◽  
Biological Nitrogen ◽  
High Seed Yield

Dry bean (Phaseolus vulgaris L.) is usually considered to be poor at biological nitrogen fixation (BNF), but large variations in this trait have been observed among bean genotypes. We evaluated 16 bean genotypes for N2 fixation ability in four N treatments: (i) uninoculated in low-N soil (30 kg N ha−1), (ii) inoculated with commercial Rhizobium leguminosarum bv. phaseoli inoculant Nitrastik-D® in low-N soil, (iii) inoculated with commercial R. leguminosarum bv. phaseoli inoculant Nodulator® in low-N soil, and (iv) uninoculated in high-N soil (100 kg N ha−1). There were differences between genotypes in all the plant parameters that were measured, but only nodulation was affected by N treatment. The 100 kg N ha−1 treatment suppressed nodulation. Seven genotypes nodulated well with either inoculant, two genotypes nodulated better with Nitrastik-D than with Nodulator, three nodulated better with Nodulator than with Nitrastik-D, and four nodulated poorly with either inoculant. Cultivars AC Redbond, Island, and Resolute, all currently commercially grown, did not fix much N2 at flowering (4–8 kg N ha−1) or maturity (19–34 kg N ha−1). By contrast, germplasm lines PI 136692 (red bean), GH-196 (pinto bean), and LEF2RB (carioca bean) had high BNF capability at flowering (10–11 kg N ha−1) and especially at maturity (60–72 kg N ha−1), in addition to high seed yield (2778–2897 kg ha−1), indicating their superior ability to support both of these economically important traits throughout plant growth. These three genotypes would be valuable to breeders for the genetic improvement of BNF in dry bean cultivars.

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

2014 ◽  
Vol 94 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Zafrin Akter ◽  
Binod B. Pageni ◽  
Newton Z. Lupwayi ◽  
Parthiba M. Balasubramanian
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Biological Nitrogen Fixation ◽  
Nifh Gene ◽  
Rapid Test ◽  
Dry Beans ◽  
Phaseolus Vulgaris L ◽  
Dry Bean ◽  
Biological Nitrogen

Akter, Z., Pageni, B. B., Lupwayi, N. Z. and Balasubramanian, P. M. 2014. Biological nitrogen fixation and nif H gene expression in dry beans ( Phaseolus vulgaris L.). Can. J. Plant Sci. 94: 203–212. Dry bean (Phaseolus vulgaris L.) is one of the least efficient nitrogen (N2) fixers among legumes (approximately 30 to 50 kg N ha−1). To identify efficient N2 fixers, 22 dry bean genotypes were screened for symbiotic N2-fixation in potted low-N soil in the greenhouse under four treatments: (1) uninoculated, (2) inoculated with commercial Rhizobium leguminosarum bv. phaseoli inoculant Nitrastik-D®, (3) inoculated with commercial Rhizobium inoculant Nodulator®, and (4) fertilizer N added. Expression of nitrogenase reductase (nifH) gene in selected uninoculated genotypes (nodulated by indigenous rhizobia) was quantified by reverse transcription polymerase chain reaction (RT-PCR) to know if expression of this gene was an indicator of N2-fixation. Nitrogen fixation potential varied among the genotypes and between the rhizobia inoculants. Genotypes differed in shoot (5.17 to 8.39 g plant−1) and root (1.71 to 6.44 g plant−1) dry weights, nodule number (0 to 155 nodules plant−1) and mass (0.055 to 0.250 mg plant−1), and N2-fixed (0 to 129 mg N plant−1). Genotypes GH–196, FR 266 and AC Redbond fixed the most N2 (60 to 112 mg N plant−1) with either rhizobial inoculant, and Viva fixed the highest amount (129 mg N plant−1) when inoculated with Nitrastik-D. In contrast, AC Black Diamond, Island, Winchester, AC Polaris, LEF 2RB and SEA 5 had low N2-fixing potential (3 to 23 mg N plant−1) in inoculated treatments. The qRT-PCR analysis indicated that nifH gene expression was 5 fold higher in GH-196 compared to the non-nodulating mutant R99, which further confirmed the high N2-fixation capacity of GH-196. After confirmation in the field, dry bean genotypes with improved N2-fixation potential identified in this study may be used as parents in breeding programs in the development of future cultivars. The nifH gene expression may be used as a rapid test to select dry beans with high N2-fixation potential.

Partitioning the contributions of biochar properties to enhanced biological nitrogen fixation in common bean (Phaseolus vulgaris)

2015 ◽  
Vol 51 (4) ◽  
pp. 479-491 ◽  
Author(s):  
David T. Güereña ◽  
Johannes Lehmann ◽  
Janice E. Thies ◽  
Akio Enders ◽  
Nancy Karanja ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Biological Nitrogen Fixation ◽  
Biological Nitrogen
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