Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.)

Euphytica ◽  
1990 ◽  
Vol 47 (3) ◽  
pp. 241-247
Author(s):  
J. Keller
Keyword(s):  
Allium Cepa ◽  
Haploid Induction ◽  
Flower Buds ◽  
Allium Cepa L

scholarly journals The genotypic response towards haploid induction in Turkish onion (Allium cepa L.) germplasm

2020 ◽  
Vol 48 (3) ◽  
pp. 1176-1184
Author(s):  
Faika YARALI KARAKAN
Keyword(s):  
Flow Cytometry ◽  
Allium Cepa ◽  
Ms Medium ◽  
Haploid Induction ◽  
Flower Buds ◽  
Plant Survival ◽  
Survival Percentage ◽  
Induction Rate ◽  
Allium Cepa L ◽  
Medium Flow

Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).

scholarly journals Embryo-gynogenic induction of Indonesia shallot (Allium cepa L. Aggregatum group) cultivars using three different protocol unpollinated flower cultures

2021 ◽  
Vol 883 (1) ◽  
pp. 012020
Author(s):  
E S Lestari ◽  
Sulastriningsih ◽  
D C Prayantini ◽  
A Purwantoro ◽  
E Sulistyaningsih
Keyword(s):  
Liquid Medium ◽  
Allium Cepa ◽  
Basic Medium ◽  
Second Step ◽  
Haploid Induction ◽  
Step Method ◽  
Allium Cepa L

Abstract Embryo-gynogenic induction was an important step in haploid induction. The research was determined the best method of unpollinated flower culture of Indonesia shallot varieties. Three methods were used including method 1 with two-step cultures using BDS as basic medium supplemented with polyamines 2 mM putrescine on the first step and spermidine 0,1 mM at the second step, method 2 using BDS as basic medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1 on the first step and NAA 1 mgL−1 and 2iP 2 mgL−1 on the second step and method 3 using B5 medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1. Six shallot cultivars used as flower donor were Katumi, Bima Brebes, Tajuk, Trisula, Superphilip, and Bauji. Flower umbel was collected and pretreated using Yoshida liquid medium then placed on incubator at four °C for overnight. The results showed that all flowers cultured could anthesis properly in the first week of cultured. The embryos could emerge from ovaries from week 7th of cultured in all methods. However, the highest percentage of embryos gynogenic developed to seedlings was achieved using method 3 in Superphilip (6.98%) followed by method 2 in Tajuk (4.44%).

Цитогенетические эффекты гамма-излучения в проростках Allium cepa L.

2018 ◽  
Vol 481 (1) ◽  
pp. 99-103
Author(s):  
А.Я. Болсуновский ◽  
Д.В. Дементьев ◽  
Е.М. Иняткина ◽  
Ю.В. Кладько ◽  
М.В. Петриченков ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Allium Cepa L

Biased chloroplast and mitochondrial transmission in somatic hybrids of Allium ampeloprasum L. and Allium cepa L.

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 219-228 ◽  
Author(s):  
J Buiteveld ◽  
W Kassies ◽  
R Geels ◽  
M.M van Lookeren Campagne ◽  
E Jacobsen ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Somatic Hybrids ◽  
Allium Ampeloprasum ◽  
Allium Cepa L ◽  
Mitochondrial Transmission
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