Development of Innovative Gel Sunscreen Products from Momordica cochinchinensis (Lour.) Spreng. Extract

A study on the development of sunscreen gel products from Momordica cochinchinensis(Lour.) Spreng. extract aimed to study phenolic content, inhibitory effect of Elastase and Tyrosinase, product stability, toxicity, astringent effect of M. cochinchinensis extract, skin elasticity value, suitable product formula calculation for preparing sunscreen gel products from M. cochinchinensis extract, and irritation test. The process started fromthe selection of raw materials, preparation of extracts for determining the total phenolic content, development of suitable formula, test of safety and product physical characteristics, and then test of the anti-allergic effect of 10 volunteers to get efficient and safe sunscreen gel from M. cochinchinensis extract. The study result indicated that M. cochinchinensisaril extract had antioxidant activity DPPH of 1.51±0.05 mg/ml, compared to standard substance - Vitamin C, and total phenolic content of 13.18±0.18 (mg equivalent of gallic acid per 100 g - dry weight). Regarding Cytotoxicity at a concentration of 0.0001-1 mg/ml, it revealed that M. cochinchinensisaril extract was not toxic to human skin cells with the cell survival percentage at a concentration of 1 mg/ml equaled to 95.35±1.86 and 88.15±4.73%, respectively. M. cochinchinensisaril extract concentration of 1 mg/ml had astringent effect which can stimulate human skin cells to move together faster than the control group but showed effect slower than Vitamin C concentrate of 1 mg/ml. and did not have inhibitory effect on Elastase and Tyrosinaseenzymes. Regarding M. cochinchinensis seed oil extract, it did not toxic to human skin cells at the concentration of 0.0001-1 mg/ml with the survival percentage equaled to 105.67-111.46%, and had a few antioxidants activity of unsaturated fatty acids with an IPC50 more than 1000 mg/ml. This study was only the development of sunscreen gel products from M. cochinchinensis extract.

A STUDY OF CORRELATION OF PRAGMATIC RISK SCORE WITH MORTALITY IN PATIENTS ADMITTED TO RLJH AND RC ICU WITH CORONAVIRUS DISEASE 2019 (COVID-19)

Background : In many people the disease is mild and self-limiting, but in a considerable portion of patients the disease is severe and fatal. Determining which patients are at high risk of severe illness or mortality is an essential part of understanding this illness Aims and Objective : Study of correlation of prognostic risk score with mortality in patients admitted to RLJH and RC ICU with coronavirus disease 2019 (COVID-19) . Methodology : This was a Retrospective observational study in the patients tested RT-PCR positive for COVID 19 and admitted at SRI DEVARAJ URS MEDICAL COLLEGE Tamaka, Kolar during the two month duration i.e. June 2021 to July 2021 in the 50 patients .The statistical analysis was done by Graph pad prism 6 version from that we have calculated correlation co-efcient and Survival graph Result : In our study we have seen that The majority of the patients were in the age group of 61-70 were 28% , followed by 51-60 were 24%, 71-80 were 22%, 41-50 were 14% , 30-40 & >80 were 6%. The majority of the patients were Male i.e. 68% and Female were 32%. There was strong correlation between Prognostic score and mortality of the patients which was statistically highly signicant i.e. Spearman r= 0.8450, P value (two-tailed) was < 0.0001*** . survival rate for the Score 0, 2 was almost 100% and as the score increases i.e. 3,4,5,6 there survival percentage also decrease approximately to 25 %, 5% and 0%,0% respectively. Conclusion :It can be concluded from our study that there was signicantly higher coreltaion between the Prognostic score and mortality of the patients hence this score could be reliable tool for the assessment of the severe patients and prompt and aggressive treatment for the better outcome

Effects of Phytohormones and Alternative Sources on the Propagation of Ternstroemia cameroonensis Cheek. by Marcotting in the Lebialem Highlands, Cameroon

Marcotting is a method of vegetative propagation which still finds its relevance in the present day forest management and routine practice to produce viable seedlings for the regeneration of Ternstroemia cameroonensis. There is dearth of information regarding the propagation of T. cameroonensis by marcotting. Hence effects of phytohormone application (Indole-3-acetc acid, Indole-3-butyric acid) and alternative sources (coconut water) on the propagation of T. cameroonensis by marcotting were investigated in the Lebialem Highlands. Mortality rates, number of roots, root length as well as marcotts position in the crown was evaluated. The propagation trials were carried under natural environmental conditions. Marcotts had an overall survival percentage of 35.41%. Those established at the middle of the crown had the highest survival percentage (15.27%) followed by those at the lower (11.80 %). Also marcotts established on branches with larger diameter (4-6 cm) and shorter length (50 cm) had the highest survival percentage (20.13 and 19.44 respectively). Again marcotts treated with IBA and CW had the best performance compared to other pre-treatments. According to the result, T. cameroonensis can be amenable through marcotts.

The Crossbreed Invariant Optimization MSVM Method for Effective Diagnosis of Pneumonia from Chest X-Ray Images

The survival percentage of pulmonary sufferers can be improved if pneumonia is detected in time. Imaging of the chest x-Ray is the most common way of finding as well as identifying pneumonia. A competent radiologist poses a severe problem while identifying pneumonia using CXR scans. To maximize classification precision, it requires an autonomous computer-aided detection approach. Designing a lightweight autonomous pneumonia detection mechanism for resource-efficient healthcare devices is critical for enhancing healthcare quality while lowering expenses and increasing reaction time. In this proposed work, a machine learning-based hybridization approach is implemented for the identification of pneumonia in the chest x-Ray scans. The proposed methodology is divided into different segments: the 1st segment is to remove noise from the chest x-Ray scans (pre-processing). After the pre-processing of CXR scans, the second module is to extract features from the pre-processed scans. The scale-invariant feature transform (SIFT) method is implemented for the extraction of essential features. This CIO-MSVM (Crossbreed Invariant Optimization-MSVM) method will select the valuable feature with the help of FF (fitness function). This function will help to select the feature matrix and then implement the MSVM algorithm. It will pass the instance selected feature set to the train model and test model. It will classify the feature sets. If feature sets will match then detect or classify the Chest X-ray image and evaluate the performance metrics such as accuracy, spec, sens., etc and compared with the existing methods.

Multi-layer Budding of Oversized Rubber (Hevea brasiliences) Seedlings: Success and Growth in Different Soil Media

The study on Multi-layer budding of oversized rubber seedling aimed at evaluating the success of multiple budding operation on single seedling of rubber tree species comprising of 4 to 7 budding layers per seedling. The experiment used randomized complete blocked design layout with 3 treatments such as 4, 5 and 7 layer budding in a single seedling replicated 4 times. Results revealed that the highest number of budding layers succeeded significantly higher as compared to 4 and 5 layers. The main survival percentage is about 79.17% for the 7 layers, 76.52% for the 5 layers and only about 55.36% for the 4 layers. Pure garden soil reported to have significant results on sprouting rate, sprout length, stalk diameter and number of leaves. This finding emphasized that budding of rejected oversized rubber seedlings grown in an abandoned nurseries can be reutilized for mass production of budded cuttings through multiple budding of 7 layers per seedlings to maximize planting materials derived from single seedlings.

Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been identified, however the functions of most of these ageing-related proteins are not known. Here, we report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. Three of the CLS-extending proteins, Ptc4, Zwf1, and Sme1, contributed to an overall higher survival percentage and shorter doubling time of yeast growth compared to the control. The CLS-extending proteins contributed to thermal and oxidative stress responses differently, suggesting different mechanisms of actions. The overexpression of Ptc4 or Zwf1 also promoted rapid cell proliferation during yeast growth, suggesting their involvement in cell division or growth pathways.

To Study the Effect of Auxin Concentrations (IBA and IAA) on Growth Performance of Stem Cutting of Hardwickia binata (Roxb.)

The present studies on “Effect of Plant Growth Regulators on Growth Performance of Stem Cutting of Hardwickia binata Roxb” was carried out in the mist chamber of Institute of Forest Biodiversity, Dulapally, Kompally, Hyderabad, during January- April, 2021. The cuttings of Hardwickia binata Roxb were prepared from selected superior phenotypes from the surrounding environments of Kompally, Hyderabad. Hardwickia binata Roxb. is difficult to root and need auxin treatment and controlled environment to successful survival and rooting. The cuttings were treated with 0, 200, 500,1000, 1500, 2000 mg/l concentrations of IBA and IAA and planted in poly bags under mist chamber. The experiment was conducted in a completely randomized design. Analysis of variance was worked out to assess the variation in Hardwickia binata Roxb. for eight characters. Treatment with IBA at 2000mg was found to be the best treatment as it achieved maximum in survival percentage (52.64%), shoot length (31.92cm), rooting percentage (81.69%), number of roots per cutting (20.44) and root biomass (6.32g) followed by treatment with IAA at 2000mg/l (T10) achieved maximum. The performance increased with an increase in concentrations of both IBA and IAA. Among the two auxins treatments, IBA was found to be most effective, showed significantly higher values compared to IAA. It is possible to successfully multiply the Hardwickia binata Roxb. cuttings by treating with 2000 mg/l of IBA under controlled phyto-environmental condition. The study evolved an easy and efficient protocol for vegetative propagation of HardwickiabinataRoxb.via stem cuttings to establish clonal forestry.

Influence of Cavity Size on the Survival of Single Surface Atraumatic Restorative Treatment Using Glass Ionomer Cement with or without Chlorhexidine Diacetate—A Randomized Trial

The purpose of the present study was to assess the influence of cavity size on the survival of conventional and CHX modified GIC in single surface primary molars receiving Atraumatic Restorative Treatment (ART). A randomized controlled trial with a split-mouth design was conducted on 90 children with symmetrical bilateral single surface carious lesions on primary molars. The teeth were randomly allotted to the conventional GIC group (group 1, n = 90) and CHX modified GIC group (group 2, n = 90). Both groups received atraumatic restorative treatment under rubber dam isolation. The cavity size was measured in terms of depth, mesiodistal, and buccolingual dimensions. The survival of ART restorations was measured after 6, 12, 18, and 24 months. The difference in proportion was tested using the Kruskal–Wallis H test, and survival curve estimation was carried out using the Kaplan–Meier method. The overall survival of all ART restorations was 83.3% at 24 months for the total sample. The survival of conventional GIC at 24 months was 83.9%, and for CHX-modified GIC was 82.7% (p > 0.05). The collective overall success of 65.1% was seen in the cavity volume category of 10–29.9 mm3. CHX modified GIC showed high survival percentage (60%) with depth >3 mm. To conclude, no significant difference was observed in the overall survival percentage of conventional and CHX modified GIC. Survival percentage was highest for cavities with a volume of 10–19.9 mm3.

358. Early Cardiac Marker of Mortality in COVID-19

Background Epicardial adipose tissue (EAT) is a highly inflammatory depot of fat, with high concentrations of IL-6 and macrophages, which can directly reach the myo-pericardium via the vasa vasorum or paracrine pathways. TNF-α and IL-6 diminish cardiac inotropic function, making EAT inflammation a potential cause of cardiac dysfunction. Methods A retrospective cohort study assessing EAT Thickness and Density from CT scans, without contrast, from adult patients during index admission for COVID-19 infection at Mount Sinai Medical Center from March 2020 to January 2021. A total of 1,644 patients were screened, of which 148 patients were included. Follow-up completed until death or discharge. The descriptive analysis was applied to the general population, parametric test of normality for comparisons between groups. Kaplan survival analysis was conducted after survival distribution was confirmed significant. It was followed by the assumption of normality by Q-Q Plot, prior to performing a multiple regression analysis in the vulnerable group using a K-Matrix input for cofounders. A log-rank test was conducted to determine differences in the survival distributions for the different ranges of EAT thickness. Results A total of 148 Participants were assigned to two groups based on epicardial adipose tissue in order to classify them as increased or decreased risk of cardiovascular risk: &gt;5mm (n = 99), &lt; 5mm (n = 49). The survival percentage was higher in the group with no EAT inflammation compared to the group with EAT inflammation (95.0% and 65%, respectively). Participants with EAT &gt;5mm had a median day of hospital stay of 18 (95% CI, 16.86 to 29.92). The survival distributions for the two categories were statistically significantly different, χ2(2) = 6.9, p &lt; 0.01. A Bonferroni correction was made with statistical significance accepted at the p &lt; 0.025 level. There was a statistically significant difference in survival distributions for the EAT &gt;5 mm vs EAT &lt; 5 mm, χ2(1) =6.953, p = 0.008. EAT Thickness Survival Analysis 2020-2021 COVID-19 MSMC Scatter Plot Length of Stay by EAT Thickness Conclusion There was an association with increased EAT thickness and increased mortality. These findings suggest that EAT thickness can be used as a prognostic factor and as a risk factor for increased mortality in patients with COVID-19 Disclosures All Authors: No reported disclosures

Effect of Phytohormones on rooting behaviour in air layers of Phoebe cooperiana, an economically important tree species of Arunachal Pradesh, India

This study reports the response of air layers of Phoebe cooperiana (Common name: Tapil) to different concentrations of rooting hormones in mid hills of Arunachal Pradesh. Ten trees between the ages of 7 and 10 were selected at Boleng village of Siang district, Arunachal Pradesh, India for the experiment. Eight treatments comprising of IBA and NAA at concentrations of 1000ppm, 2000ppm and 3000ppm each, Rootex hormone for semi-hardwood and control were applied to a total of 240 layers during April 2020. After 60 days, rooting percentage, mean number of roots and length of the longest root were recorded. Significant variation (p<0.05) among treatments was observed for all root parameters. Among treatments, the highest rooting percentage and mean a number of roots were obtained in layers treated with NAA 3000ppm (80% and 21.33 respectively). The length of the longest root was the highest in layers treated with NBA 2000ppm (4.23cm). The highest survival percentage of layers two months after transplanting was observed in those treated with NAA 3000ppm (54.16%) and the least in control (23.07%). The technique offers an easy and cheap method of propagation for farmers as well as for government and private agencies to enhance seedling production of the species.