The genotypic response towards haploid induction in  Turkish onion (Allium cepa L.) germplasm

Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).

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Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.)

Euphytica ◽

10.1007/bf00024247 ◽

1990 ◽

Vol 47 (3) ◽

pp. 241-247

Author(s):

J. Keller

Keyword(s):

Allium Cepa ◽

Haploid Induction ◽

Flower Buds ◽

Allium Cepa L

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Callus regeneration and polyploidy induction of Allium cepa L var. Bima Brebes using oryzalin

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/948/1/012043 ◽

2021 ◽

Vol 948 (1) ◽

pp. 012043

Author(s):

R Q A’ yun ◽

D Dinarti ◽

A Husni ◽

M Kosmiatin

Keyword(s):

Flow Cytometry ◽

Allium Cepa ◽

Ploidy Level ◽

Dna Content ◽

Callus Induction ◽

Consumer Preference ◽

Morphological Trait ◽

Flow Cytometry Analysis ◽

Allium Cepa L ◽

Callus Regeneration

Abstract Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytometry. The results showed callus diameter, number of green spots, and number of shoots were decreased with increasing oryzalin concentration. The planlet leaves regenerated from oryzalin treated callus were darker than that of control. The flow cytometry analysis showed that planlets with 75 μM oryzalin was tetraploid, had longer and wider stomata than that of the control.

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Combate de fitopatógenos en almácigo de cebolla (Allium cepa L.).

Agronomía Mesoamericana ◽

10.15517/am.v8i2.24671 ◽

2016 ◽

Vol 8 (2) ◽

pp. 107

Author(s):

Juan Ramón Navarro ◽

Gerardina Umaña

Keyword(s):

Costa Rica ◽

Allium Cepa ◽

Weather Conditions ◽

Plant Survival ◽

Root Pathogens ◽

Allium Cepa L ◽

Control Root ◽

Compost Treatment

The effect of several treatments to control root pathogens on onion seedlings (Allium cepa L.) were studied. The test was carried out in Bagaces, Guanacaste province, Costa Rica. Treat-ments tested were: Benomil + Captan, solarization for three weeks ,solarization for four weeks , compost at arate of 25 t/ha, and a control. During the test weather conditions were exceptionally wet thus increasing the pathogen pressure beyond normal. Despite weather conditions, the compost treatment showed the highest plant survival and seedlings. The use of compost seems to be an adequate altemative for the production of onion seedlings.

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Allium cepa L. Cultivars from Four Continents Compared by Flow Cytometry show Nuclear DNA Constancy

Annals of Botany ◽

10.1006/anbo.1999.1071 ◽

2000 ◽

Vol 85 (3) ◽

pp. 351-357 ◽

Cited By ~ 33

Author(s):

M Bennett

Keyword(s):

Flow Cytometry ◽

Allium Cepa ◽

Nuclear Dna ◽

Allium Cepa L

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Embryo-gynogenic induction of Indonesia shallot (Allium cepa L. Aggregatum group) cultivars using three different protocol unpollinated flower cultures

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/883/1/012020 ◽

2021 ◽

Vol 883 (1) ◽

pp. 012020

Author(s):

E S Lestari ◽

Sulastriningsih ◽

D C Prayantini ◽

A Purwantoro ◽

E Sulistyaningsih

Keyword(s):

Liquid Medium ◽

Allium Cepa ◽

Basic Medium ◽

Second Step ◽

Haploid Induction ◽

Step Method ◽

Allium Cepa L

Abstract Embryo-gynogenic induction was an important step in haploid induction. The research was determined the best method of unpollinated flower culture of Indonesia shallot varieties. Three methods were used including method 1 with two-step cultures using BDS as basic medium supplemented with polyamines 2 mM putrescine on the first step and spermidine 0,1 mM at the second step, method 2 using BDS as basic medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1 on the first step and NAA 1 mgL−1 and 2iP 2 mgL−1 on the second step and method 3 using B5 medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1. Six shallot cultivars used as flower donor were Katumi, Bima Brebes, Tajuk, Trisula, Superphilip, and Bauji. Flower umbel was collected and pretreated using Yoshida liquid medium then placed on incubator at four °C for overnight. The results showed that all flowers cultured could anthesis properly in the first week of cultured. The embryos could emerge from ovaries from week 7th of cultured in all methods. However, the highest percentage of embryos gynogenic developed to seedlings was achieved using method 3 in Superphilip (6.98%) followed by method 2 in Tajuk (4.44%).

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250 INDUCTION AND AVOIDANCE OF VITRIFICATION OF MICROPROPAGATED ONION (ALLIUM CEPA L.)

HortScience ◽

10.21273/hortsci.29.5.465d ◽

1994 ◽

Vol 29 (5) ◽

pp. 465d-465

Author(s):

Renee M. Schloupt ◽

Walter E. Splittstoesser ◽

Robert M. Skirvin

Keyword(s):

Liquid Medium ◽

Allium Cepa ◽

Shoot Tips ◽

Shoot Tip ◽

Ms Medium ◽

Allium Cepa L ◽

Micropropagated Plants ◽

Shoot Tip Explants

The objective of this research was to induce vitrification in onion (Allium cepa L. cv. `White Ebeneezer'); then use this information to make suggestions on how to avoid vitrification of micropropagated plants. There were no differences in vitrification percentage when shoot tip explants were isolated, sterilized and placed on MS medium (8 g.L-1 agar) supplemented with 0.16 uM NAA and varying (0.0 to 70.0 uM) levels of BA. When agar was replaced by gelrite (MS medium with 4.4 uM BA and 0.16 uM NAA), vitrification increased when gelrite concentrations decreased from 2.0 to 1.0 g.L-1. More vitrification occurred when shoot tips were supported on a synthetic cosmetic puff in liquid medium or when agar was reduced to 4.0 g.L-1 than when supported on a cosmetic puff in 8 g.L-1 agar or on 8 g.L-1 agar alone.

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