Purification and characterization of 8 of the pathogenesis-related proteins in tobacco leaves reacting hypersensitively to tobacco mosaic virus

1986 ◽  
Vol 6 (2) ◽  
pp. 69-80 ◽  
Author(s):  
Elisabeth Jamet ◽  
Bernard Fritig
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Purification And Characterization ◽  
Tobacco Leaves ◽  
Pathogenesis Related Proteins ◽  
Pathogenesis Related ◽  
Related Proteins

scholarly journals Synthesis and localization of pathogenesis-related proteins in tobacco.

1987 ◽  
Vol 7 (4) ◽  
pp. 1580-1583 ◽  
Author(s):  
J P Carr ◽  
D C Dixon ◽  
B J Nikolau ◽  
K V Voelkerding ◽  
D F Klessig
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Immunofluorescence Microscopy ◽  
Pathogenesis Related Proteins ◽  
Chemical Agents ◽  
Nicotiana Tabacum L ◽  
Pathogenesis Related ◽  
Major Cell Type ◽  
Xylem Elements ◽  
Related Proteins

The PR1 family of pathogenesis-related proteins from tobacco (Nicotiana tabacum L.) leaves is induced by a variety of pathogenic and chemical agents and is associated with resistance to tobacco mosaic virus. The majority of the PR1 proteins did not copurify with mesophyll protoplasts (the major cell type of the leaf) isolated from tobacco mosaic virus-infected N. tabacum cv. Xanthi-nc leaves. However, these isolated protoplasts were capable of synthesizing and selectively secreting the PR1 proteins. Using monoclonal antibodies for immunofluorescence microscopy, we localized these proteins to the extracellular spaces predominantly in regions adjacent to viral lesions as well as in xylem elements of infected leaves.

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