Somatic embryogenesis and plant regeneration from callus cultures of Aconitum heterophyllum Wall

1993 ◽  
Vol 32 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Archana Giri ◽  
Paramvir Singh Ahuja ◽  
P. V. Ajay Kumar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures ◽  
Aconitum Heterophyllum

Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus Tricyrtis

2004 ◽  
Vol 40 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Masaru Nakano ◽  
Keiko Mizunashi ◽  
Shigefumi Tanaka ◽  
Toshinari Godo ◽  
Masashi Nakata ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures

Plant regeneration by somatic embryogenesis from callus cultures of sweet sorghum

1986 ◽  
Vol 5 (5) ◽  
pp. 349-351 ◽  
Author(s):  
Christy MacKinnon ◽  
Glenys Gunderson ◽  
Murray W. Nabors
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Sweet Sorghum ◽  
Callus Cultures

EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS FROM IMMATURE LEAF-DERIVED PROTOPLASTS OF GROUNDNUT (ARACHIS HYPOGAEA L.)

1996 ◽  
Vol 44 (4) ◽  
pp. 387-396 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Narayanasamypillai Jayabalan
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Callus Cultures ◽  
Alternative Methods ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
High Yields ◽  
2,4 Dichlorophenoxyacetic Acid

High yields of protoplasts were obtained from immature leaves of aseptically grown plants of Arachis hypogaea using an enzyme solution containing cellulase 2.0% (w/v) and Macerozyme 1.0% (w/v) in 0.6 M mannitol. Isolated protoplasts were cultured in Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The protoplasts started to divide after 3–5 days of culture. Sustained divisions resulted in mass production of cell colonies and mini calli in 4 weeks. After 4 weeks, protoplast colonies were transferred to the Murashige and Skoog (MS) medium supplemented with a-naphthalene acetic acid (NAA) and BAP. Colonies proliferated into actively growing calli. Further attempts to regenerate plants from such calli were not successful. However, protoclones differentiated roots on the same medium. Alternative methods for plant regeneration from protoplast derived callus cultures were tried through somatic embryogenesis. Protoplast-derived calli treated with 2,4-D and BAP formed somatic embryos. Somatic embryogenesis began in the proembryo stage and proceeded from globular to dicotyledonary stage. Embryos were then transferred onto hormone-free MS medium for germination. Five to ten percent of these embryoids germinated and grew to plantlets. Regenerated plants were transferred to plastic cups and grown to maturity.

Plant regeneration via organogenesis and somatic embryogenesis in callus cultures derived from mature zygotic embryos of leek (Allium ampeloprasum L.)

Euphytica ◽  
1996 ◽  
Vol 91 (3) ◽  
pp. 261-270 ◽  
Author(s):  
B. Silvertand ◽  
A. van Rooyen ◽  
P. Lavrijsen ◽  
A. M. van Harten ◽  
E. Jacobsen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures ◽  
Zygotic Embryos ◽  
Allium Ampeloprasum

scholarly journals Somatic Embryogenesis and Plant Regeneration in Basal Plate and Receptacle Derived-Callus Cultures of Garlic (Allium sativum L.).

1991 ◽  
Vol 60 (3) ◽  
pp. 627-634 ◽  
Author(s):  
Hui-min Xue ◽  
Hajime Araki ◽  
Ling Shi ◽  
Toshiro Yakuwa
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Allium Sativum ◽  
Callus Cultures ◽  
Basal Plate

Somatic Embryogenesis and Plant Regeneration from Callus Cultures of ‘Tifton 9’ Bahiagrass

Crop Science ◽  
1994 ◽  
Vol 34 (5) ◽  
pp. 1378-1384 ◽  
Author(s):  
Robert G. Shatters ◽  
Richard A. Wheeler ◽  
S. H. West
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures
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