Somatic embryogenesis and plant regeneration in inflorescence and seed derived callus cultures of Poa pratensis L. (Kentucky bluegrass)

1989 ◽  
Vol 7 (8) ◽  
pp. 644-647 ◽  
Author(s):  
P. van der Valk ◽  
M. A. C. M. Zaal ◽  
J. Creemers-Molenaar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Callus Cultures

Somatic embryogenesis and plant regeneration from callus cultures of Aconitum heterophyllum Wall

1993 ◽  
Vol 32 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Archana Giri ◽  
Paramvir Singh Ahuja ◽  
P. V. Ajay Kumar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures ◽  
Aconitum Heterophyllum

Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus Tricyrtis

2004 ◽  
Vol 40 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Masaru Nakano ◽  
Keiko Mizunashi ◽  
Shigefumi Tanaka ◽  
Toshinari Godo ◽  
Masashi Nakata ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures

Plant regeneration by somatic embryogenesis from callus cultures of sweet sorghum

1986 ◽  
Vol 5 (5) ◽  
pp. 349-351 ◽  
Author(s):  
Christy MacKinnon ◽  
Glenys Gunderson ◽  
Murray W. Nabors
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Sweet Sorghum ◽  
Callus Cultures

EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS FROM IMMATURE LEAF-DERIVED PROTOPLASTS OF GROUNDNUT (ARACHIS HYPOGAEA L.)

1996 ◽  
Vol 44 (4) ◽  
pp. 387-396 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Narayanasamypillai Jayabalan
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Callus Cultures ◽  
Alternative Methods ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
High Yields ◽  
2,4 Dichlorophenoxyacetic Acid

High yields of protoplasts were obtained from immature leaves of aseptically grown plants of Arachis hypogaea using an enzyme solution containing cellulase 2.0% (w/v) and Macerozyme 1.0% (w/v) in 0.6 M mannitol. Isolated protoplasts were cultured in Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The protoplasts started to divide after 3–5 days of culture. Sustained divisions resulted in mass production of cell colonies and mini calli in 4 weeks. After 4 weeks, protoplast colonies were transferred to the Murashige and Skoog (MS) medium supplemented with a-naphthalene acetic acid (NAA) and BAP. Colonies proliferated into actively growing calli. Further attempts to regenerate plants from such calli were not successful. However, protoclones differentiated roots on the same medium. Alternative methods for plant regeneration from protoplast derived callus cultures were tried through somatic embryogenesis. Protoplast-derived calli treated with 2,4-D and BAP formed somatic embryos. Somatic embryogenesis began in the proembryo stage and proceeded from globular to dicotyledonary stage. Embryos were then transferred onto hormone-free MS medium for germination. Five to ten percent of these embryoids germinated and grew to plantlets. Regenerated plants were transferred to plastic cups and grown to maturity.

scholarly journals 582 PB 091 PLANT REGENERATION FROM EMBRYOGENIC CALLUS TISSUES OF KENTUCKY BLUEGRASS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515c-515
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee
Keyword(s):  
Plant Regeneration ◽  
Light Exposure ◽  
Poa Pratensis ◽  
Shoot Proliferation ◽  
Kentucky Bluegrass ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Albino Plants ◽  
Proliferation Medium ◽  
Callus Tissues

Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5-2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2,4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2,4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15-0.3 mg/liter picloram or 0.2-0.5 mg/liter 2,4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5 mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks.

Plant regeneration via organogenesis and somatic embryogenesis in callus cultures derived from mature zygotic embryos of leek (Allium ampeloprasum L.)

Euphytica ◽  
1996 ◽  
Vol 91 (3) ◽  
pp. 261-270 ◽  
Author(s):  
B. Silvertand ◽  
A. van Rooyen ◽  
P. Lavrijsen ◽  
A. M. van Harten ◽  
E. Jacobsen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures ◽  
Zygotic Embryos ◽  
Allium Ampeloprasum
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