Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population

1996 ◽  
Vol 2 (1) ◽  
Author(s):  
Hong-Bin Zhang ◽  
Sangdun Choi ◽  
Sung-Sick Woo ◽  
Zhikang Li ◽  
RodA. Wing
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Recombinant Inbred ◽  
Mapping Population ◽  
Artificial Chromosome

scholarly journals Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle α-actin gene

genesis ◽  
2010 ◽  
Vol 48 (7) ◽  
pp. 457-463 ◽  
Author(s):  
John J. Armstrong ◽  
Irina V. Larina ◽  
Mary E. Dickinson ◽  
Warren E. Zimmer ◽  
Karen K. Hirschi
Keyword(s):  
Smooth Muscle ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Artificial Chromosome ◽  
Actin Gene

Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library

Genomics ◽  
2000 ◽  
Vol 65 (2) ◽  
pp. 87-94 ◽  
Author(s):  
Marie-Christine Le Paslier ◽  
Raymond J. Pierce ◽  
Françoise Merlin ◽  
Hirohisa Hirai ◽  
Wenjie Wu ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Schistosoma Mansoni ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome

Construction and characterization of a new bovine bacterial artificial chromosome library with 10 genome-equivalent coverage

2000 ◽  
Vol 11 (8) ◽  
pp. 662-663 ◽  
Author(s):  
Wesley Warren ◽  
Timothy P.L. Smith ◽  
Caird E. Rexroad III ◽  
Scott C. Fahrenkrug ◽  
Tanya Allison ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome ◽  
Genome Equivalent

Construction and Characterization of a Deep-Coverage Bacterial Artificial Chromosome Library for Maize

Crop Science ◽  
2002 ◽  
Vol 42 (3) ◽  
pp. 928 ◽  
Author(s):  
J. P. Tomkins ◽  
G. Davis ◽  
D. Main ◽  
Y. Yim ◽  
N. Duru ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome

Construction and characterization of a duck bacterial artificial chromosome library

2006 ◽  
Vol 37 (6) ◽  
pp. 599-600 ◽  
Author(s):  
X. Yuan ◽  
M. Zhang ◽  
W. Ruan ◽  
C. Song ◽  
L. Ren ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome

scholarly journals Genotypic characterization of two bacterial artificial chromosome clones derived from a single DNA source of the very virulent gallid herpesvirus-2 strain C12/130

2011 ◽  
Vol 92 (7) ◽  
pp. 1500-1507 ◽  
Author(s):  
Stephen J. Spatz ◽  
Lorraine P. Smith ◽  
Susan J. Baigent ◽  
Lawrence Petherbridge ◽  
Venugopal Nair
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Genetic Changes ◽  
Mixed Genotype ◽  
Bac Clones ◽  
Gallid Herpesvirus 2 ◽  
The Individual

The identification of specific genetic changes associated with differences in the pathogenicity of Marek's disease virus strains (GaHV-2) has been a formidable task due to the large number of mutations in mixed-genotype populations within DNA preparations. Very virulent UK isolate C12/130 induces extensive lymphoid atrophy, neurological manifestations and early mortality in young birds. We have recently reported the construction of several independent full-length bacterial artificial chromosome (BAC) clones of C12/130 capable of generating fully infectious viruses with significant differences in their pathogenicity profiles. Two of these clones (vC12/130-10 and vC12/130-15), which showed differences in virulence relative to each other and to the parental strain, had similar replication kinetics both in vitro and in vivo in spite of the fact that vC12/130-15 was attenuated. To investigate the possible reasons for this, the nucleotide sequences of both clones were determined. Sequence analysis of the two genomes identified mutations within eight genes. A single 494 bp insertion was identified within the genome of the virulent vC12/130-10 clone. Seven non-synonymous substitutions distinguished virulent vC12/130-10 from that of attenuated vC12/130-15. By sequencing regions of parental DNA that differed between the two BAC clones, we confirmed that C12/130 does contain these mutations in varying proportions. Since the individual reconstituted BAC clones were functionally attenuated in vivo and derived from a single DNA source of phenotypically very virulent C12/130, this suggests that the C12/130 virus population exists as a collection of mixed genotypes.

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