Captan binding to avian myeloblastosis virus reverse transcriptase and its effect on RNase H activity

1990 ◽  
Vol 94 (1) ◽  
Author(s):  
M.J. Freeman-Wittig ◽  
RogerA. Lewis
Keyword(s):  
Reverse Transcriptase ◽  
Rnase H ◽  
Avian Myeloblastosis Virus

scholarly journals Mn2+ Suppressor Mutations and Biochemical Communication between Ty1 Reverse Transcriptase and RNase H Domains

2007 ◽  
Vol 81 (17) ◽  
pp. 9004-9012 ◽  
Author(s):  
Robert M. Yarrington ◽  
Jichao Chen ◽  
Eric C. Bolton ◽  
Jef D. Boeke
Keyword(s):  
Reverse Transcriptase ◽  
Ion Homeostasis ◽  
Point Mutations ◽  
Rnase H ◽  
Avian Myeloblastosis Virus ◽  
Manganese Ion ◽  
Suppressor Mutations ◽  
Suppressor Mutants ◽  
Ty1 Retrotransposition

ABSTRACT Ty1 reverse transcriptase/RNase H (RT/RH) is exquisitely sensitive to manganese concentrations. Elevated intracellular free Mn2+ inhibits Ty1 retrotransposition and in vitro Ty1 RT-polymerizing activity. Furthermore, Mn2+ inhibition is not limited to the Ty1 RT, as this ion similarly inhibits the activities of both avian myeloblastosis virus and human immunodeficiency virus type 1 RTs. To further characterize Mn2+ inhibition, we generated RT/RH suppressor mutants capable of increased Ty1 transposition in pmr1Δ cells. PMR1 codes for a P-type ATPase that regulates intracellular calcium and manganese ion homeostasis, and pmr1 mutants accumulate elevated intracellular manganese levels and display 100-fold less transposition than PMR1 + cells. Mapping of these suppressor mutations revealed, surprisingly, that suppressor point mutations localize not to the RT itself but to the RH domain of the protein. Furthermore, Mn2+ inhibition of in vitro RT activity is greatly reduced in all the suppressor mutants, whereas RH activity and cleavage specificity remain largely unchanged. These intriguing results reveal that the effect of these suppressor mutations is transmitted to the polymerase domain and suggest biochemical communication between these two domains during reverse transcription.

Discrimination of DNA polymerase and RNase H activities in reverse transcriptase of avian myeloblastosis virus

Biochemistry ◽  
1978 ◽  
Vol 17 (12) ◽  
pp. 2438-2442 ◽  
Author(s):  
Marian Gorecki ◽  
Amos Panet
Keyword(s):  
Reverse Transcriptase ◽  
Dna Polymerase ◽  
Rnase H ◽  
Avian Myeloblastosis Virus

scholarly journals Mechanism of RNA primer removal by the RNase H activity of avian myeloblastosis virus reverse transcriptase.

1984 ◽  
Vol 49 (3) ◽  
pp. 686-691 ◽  
Author(s):  
J J Champoux ◽  
E Gilboa ◽  
D Baltimore
Keyword(s):  
Reverse Transcriptase ◽  
Rnase H ◽  
Avian Myeloblastosis Virus

scholarly journals RNase H activity associated with bacterially expressed reverse transcriptase of human T-cell lymphotropic virus III/lymphadenopathy-associated virus.

1987 ◽  
Vol 262 (26) ◽  
pp. 12393-12396 ◽  
Author(s):  
J Hansen ◽  
T Schulze ◽  
K Moelling
Keyword(s):  
T Cell ◽  
Reverse Transcriptase ◽  
Rnase H ◽  
Human T Cell

scholarly journals Sequence relatedness between the subunits of avian myeloblastosis virus reverse transcriptase.

1975 ◽  
Vol 250 (13) ◽  
pp. 5278-5280 ◽  
Author(s):  
H M Rho ◽  
D P Grandgenett ◽  
M Green
Keyword(s):  
Reverse Transcriptase ◽  
Avian Myeloblastosis Virus ◽  
Sequence Relatedness

scholarly journals Flavonoids and Acid-Hydrolysis derivatives ofNeo-Clerodane diterpenes fromTeucrium flavumsubsp.glaucumas inhibitors of the HIV-1 reverse transcriptase–associated RNase H function

2021 ◽  
Vol 36 (1) ◽  
pp. 749-757
Author(s):  
Benedetta Fois ◽  
Angela Corona ◽  
Enzo Tramontano ◽  
Simona Distinto ◽  
Elias Maccioni ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Acid Hydrolysis ◽  
Rnase H ◽  
Clerodane Diterpenes ◽  
Hiv 1

scholarly journals Stereochemical course of polymerization catalyzed by avian myeloblastosis virus reverse transcriptase.

1982 ◽  
Vol 257 (15) ◽  
pp. 8879-8884 ◽  
Author(s):  
P A Bartlett ◽  
F Eckstein
Keyword(s):  
Reverse Transcriptase ◽  
Avian Myeloblastosis Virus

scholarly journals Free Energy-Based Virtual Screening and Optimization of RNase H Inhibitors of HIV-1 Reverse Transcriptase

ACS Omega ◽  
2016 ◽  
Vol 1 (3) ◽  
pp. 435-447 ◽  
Author(s):  
Baofeng Zhang ◽  
Michael P. D’Erasmo ◽  
Ryan P. Murelli ◽  
Emilio Gallicchio
Keyword(s):  
Free Energy ◽  
Virtual Screening ◽  
Reverse Transcriptase ◽  
Rnase H ◽  
Hiv 1
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