Establishment of a gene tagging system in Arabidopsis thaliana based on the maize transposable element Ac

1992 ◽  
Vol 84-84 (3-4) ◽  
pp. 371-383 ◽  
Author(s):  
T. Altmann ◽  
R. Schmidt ◽  
L. Willmitzer
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Gene Tagging ◽  
Maize Transposable Element ◽  
Tagging System

scholarly journals Behaviour of the maize transposable element Ac in Arabidopsis thaliana

1992 ◽  
Vol 2 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Caroline Dean ◽  
Christina Sjodin ◽  
Tania Page ◽  
Jonathan Jones ◽  
Clare Lister
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Maize Transposable Element

Transfer of the maize transposable element MU1 into Arabidopsis thaliana

Plant Science ◽  
1987 ◽  
Vol 48 (3) ◽  
pp. 165-173 ◽  
Author(s):  
Hong Zhang ◽  
Chris R. Somerville
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Maize Transposable Element

scholarly journals Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yan Xue ◽  
Zhenhui Zhong ◽  
C. Jake Harris ◽  
Javier Gallego-Bartolomé ◽  
Ming Wang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Zinc Finger ◽  
Protein Function ◽  
C Elegans ◽  
Eukaryotic Organisms

AbstractThe Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants and animals, and C. elegans MORC-1 has been shown to topologically entrap and condense DNA. In Arabidopsis thaliana, mutation of MORCs has been shown to reactivate silent methylated genes and transposons and to decondense heterochromatic chromocenters, despite only minor changes in the maintenance of DNA methylation. Here we provide the first evidence localizing Arabidopsis MORC proteins to specific regions of chromatin and find that MORC4 and MORC7 are closely co-localized with sites of RNA-directed DNA methylation (RdDM). We further show that MORC7, when tethered to DNA by an artificial zinc finger, can facilitate the establishment of RdDM. Finally, we show that MORCs are required for the efficient RdDM mediated establishment of DNA methylation and silencing of a newly integrated FWA transgene, even though morc mutations have no effect on the maintenance of preexisting methylation at the endogenous FWA gene. We propose that MORCs function as a molecular tether in RdDM complexes to reinforce RdDM activity for methylation establishment. These findings have implications for MORC protein function in a variety of other eukaryotic organisms.

scholarly journals Characterization of the Putative Transposase mRNA of Tag1, Which Is Ubiquitously Expressed in Arabidopsis and Can Be Induced by Agrobacterium-Mediated Transformation With dTag1 DNA

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 693-701 ◽  
Author(s):  
Dong Liu ◽  
Nigel M Crawford
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Transcript Levels ◽  
Agrobacterium Mediated Transformation ◽  
Transgenic Lines ◽  
Localization Signal ◽  
Transposase Mrna

Abstract Tag1 is an autonomous transposable element of Arabidopsis thaliana. Tag1 expression was examined in two ecotypes of Arabidopsis (Columbia and No-0) that were transformed with CaMV 35S-Tag1-GUS DNA. These ecotypes contain no endogenous Tag1 elements. A major 2.3-kb and several minor transcripts were detected in all major organs of the plants. The major transcript encoded a putative transposase of 84.2 kD with two nuclear localization signal sequences and a region conserved among transposases of the Ac or hAT family of elements. The abundance of Tag1 transcripts varied among transgenic lines and did not correlate with somatic excision frequency or germinal reversion rates, suggesting that factors other than transcript levels control Tag1 excision activity. In untransformed plants of the Landsberg ecotype, which contain two endogenous Tag1 elements, no Tag1 transcripts were detected. Agrobacterium-mediated transformation of these Landsberg plants with a defective 1.4-kb Tag1 element resulted in the appearance of full-length Tag1 transcripts from the endogenous elements. Transformation with control DNA containing no Tag1 sequences did not activate endogenous Tag1 expression. These results indicate that Agrobacterium-mediated transformation with dTag1 can activate the expression of Tag1.

The Use of Mutator for Gene-Tagging: Cross-Referencing between Transposable Element Systems

1988 ◽  
pp. 137-147 ◽  
Author(s):  
N. S. Shepherd ◽  
W. F. Sheridan ◽  
M. G. Mattes ◽  
G. Deno
Keyword(s):  
Transposable Element ◽  
Gene Tagging
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