Genetic transformation of foxglove (Digitalis purpurea) by chimeric foreign genes and production of cardioactive glycosides

1990 ◽  
Vol 9 (3) ◽  
Author(s):  
Kazuki Saito ◽  
Mami Yamazaki ◽  
Koichiro Shimomura ◽  
Kayo Yoshimatsu ◽  
Isamu Murakoshi
Keyword(s):  
Genetic Transformation ◽  
Digitalis Purpurea ◽  
Foreign Genes ◽  
Cardioactive Glycosides

A Stable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of the Medicinal Plant Digitalis purpurea L.

2013 ◽  
Vol 172 (4) ◽  
pp. 1807-1817 ◽  
Author(s):  
Ying Li ◽  
Zhenrui Gao ◽  
Chunlan Piao ◽  
Kaiwen Lu ◽  
Zhiping Wang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Medicinal Plant ◽  
Genetic Transformation ◽  
Digitalis Purpurea

scholarly journals GENETIC TRANSFORMATION AND REGENERATION OF TRANSGENIC SWEETPOTATO PLANTS.

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 435f-435 ◽  
Author(s):  
Marceline Egnin ◽  
C.S. Prakash
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Binary Vector ◽  
Blot Hybridization ◽  
Pcr Amplification ◽  
Ms Medium ◽  
Petiole Explants ◽  
Efficient Delivery ◽  
Foreign Genes

This study aimed to optimize factors for the efficient delivery of foreign genes into sweetpotato using Agrobacterium tumefaciens and develop transgenic plants. Disarmed Agrobacterium C58 carrying a binary vector pBI 121C2H with gusA, nptll, and the nutritional protein asp-l genes was used to cocultivate (4 days) petiole explants of the sweetpotato genotype P1318846-3. Pre-incubation of petioles for 3 days on MS medium with 2,4-D (0.2 mg·liter–1) before infection resulted in higher transformation. Putative transgenic shoots were obtained by transfer of petioles to MS medium with TDZ (0.2 mg·liter–1) and kanamycin (80 to 140 mg·liter–1). The PCR amplification of gusA, nptll, and asp-1 genes in the 37 putative transgenic shoots showed that six plants contained the three genes. However, none of these plants showed histochemical expression of the gusA gene. The introduced gene may have been methylated resulting in the lack of its expression. DNA blot hybridization studies are underway to verify the presence and integration of the transgenes.

scholarly journals Genetic transformation of Digitalis purpurea by Agrobacterium tumefaciens

1997 ◽  
Vol 13 (1) ◽  
pp. 60-62
Author(s):  
N. Ya. Pogrebnyak ◽  
L. N. Shisha ◽  
S. V. Storozhenko ◽  
L. I. Yuzefovich ◽  
B. Dittrich ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Digitalis Purpurea

Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.

2014 ◽  
Vol 8 (5) ◽  
pp. 387-397 ◽  
Author(s):  
Naivy Pérez-Alonso ◽  
Borys Chong-Pérez ◽  
Alina Capote ◽  
Anabel Pérez ◽  
Yovanny Izquierdo ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Digitalis Purpurea

scholarly journals Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines

2008 ◽  
Vol 43 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Caroline Pereira Petrillo ◽  
Newton Portilho Carneiro ◽  
Antônio Álvaro Corsetti Purcino ◽  
Carlos Henrique Siqueira Carvalho ◽  
José Donizeti Alves ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Particle Bombardment ◽  
Inbred Lines ◽  
Zygotic Embryos ◽  
Tropical Maize ◽  
Gene Expressions ◽  
Maize Genotypes ◽  
Maize Genetic ◽  
Foreign Genes ◽  
Genetic Transformation System

The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba

Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Xiaomei Li ◽  
Liu Liu ◽  
Xiangyu Liu ◽  
Yan Hou ◽  
Bingyin Xu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Resistance Gene ◽  
Expression Vectors

RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription FactorOsMYB14

2013 ◽  
Vol 19 (6) ◽  
pp. 960
Author(s):  
Yanwen MIAO ◽  
Rong LI ◽  
Fangjie XIONG ◽  
Zhengming ZENG ◽  
Xiangli NIU ◽  
...  
Keyword(s):  
Rna Interference ◽  
Genetic Transformation ◽  
Function Analysis ◽  
Vector Construction ◽  
And Function

Construction and Genetic Transformation of TomatoARF4RNA Interference Expression Vector with Fruit Specific Promoter

2012 ◽  
Vol 18 (2) ◽  
pp. 206
Author(s):  
Yuanyuan FENG ◽  
Yingnan LI ◽  
Pei HOU ◽  
Jingrong ZHANG ◽  
Songhu WANG ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Expression Vector
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