Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.)

1994 ◽  
Vol 13 (10) ◽  
Author(s):  
K. Chengalrayan ◽  
SangeetaS. Sathaye ◽  
Sulekha Hazra
Keyword(s):  
Somatic Embryogenesis ◽  
Arachis Hypogaea ◽  
Mature Embryo ◽  
Arachis Hypogaea L

EFFECT OF 2,4-D AND NAA ON SOMATIC EMBRYOGENESIS FROM APICAL PORTION OF GROUNDNUT (ARACHIS HYPOGAEA L.)

2021 ◽  
Vol 1 (7) ◽  
pp. 119-124
Author(s):  
Muniappan V ◽  
Manivel P ◽  
Prabakaran V ◽  
Palanivel S ◽  
Parvathi S
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Arachis Hypogaea ◽  
Somatic Embryos ◽  
Mature Embryo ◽  
Induction Medium ◽  
Embryogenic Cultures ◽  
Apical Portion ◽  
Arachis Hypogaea L ◽  
Somatic Embryo Induction

Somatic embryogenesis was carried out epicotyl portion of the mature embryo/apical portion. The somatic embryo induction medium containing 2,4-D or NAA (10.0 to 50.0 mg/l). Of the two concentrations tested 2,4-D (30.0mg/l) recorded the highest percentage of response followed by NAA (30.0mg/l). But the highest number of somatic embryo were recorded in 30.0mg/l of 2,4-D followed by NAA. The apical portion of the mature embryo formed direct embryos without any intervention of callus. The maximum percentage of embryogenic cultures were noticed in 30.0mg/l of 2,4-D followed by NAA at 30.0mg/l. for the differentiation of somatic embryos, the embryogenic masses were transferred to medium without any growth regulator. The maximum number of somatic embryos per culture was recorded in 30 mg/l of 2,4-D followed by 30.0 mg/l of NAA. Keywords: Arachis hypogaea L.,Somatic Embryogenesis, 2,4-D and NAA

Somatic embryogenesis in Arachis hypogaea L.: genotype comparison

Plant Science ◽  
1992 ◽  
Vol 83 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Peggy Ozias-Akins ◽  
William F. Anderson ◽  
C.Corley Holbrook
Keyword(s):  
Somatic Embryogenesis ◽  
Arachis Hypogaea ◽  
Arachis Hypogaea L

scholarly journals INDUKSI KALUS KACANG TANAH (Arachis hypogaea L.) VARIETAS KELINCI DENGAN PERLAKUAN 2,4-D DAN BAP

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Ida Royani ◽  
Lalu Zulkifli ◽  
Prapti Sedijani
Keyword(s):  
Somatic Embryogenesis ◽  
Statistical Analysis ◽  
Arachis Hypogaea ◽  
Callus Induction ◽  
Callus Formation ◽  
Ms Medium ◽  
Initiation Time ◽  
Arachis Hypogaea L

Callus induction determines the successfulness of micropropagation through somatic embryogenesis. This research was conducted to determine the effect of 2,4-D and BAP on callus induction of peanut (Kelinci strain). Callus induction was done on MS medium, with the observed variables were of initiation time, diameter, texture and color callus. Statistical analysis showed that 2,4-D and BAP affect the initiation time, diameter, texture and color callus. The earliest initiation of callus formation was obtained from MS medium with the addition of 4 mg/L 2,4-D. The diameter of the largest callus obtained from MS medium with the addition of 3 mg/L 2,4-D + 1 mg/L BAP. Callus color were affected by the treatment of 2,4-D and BAP, while callus texture was not affected by these treatmen, in which compact callus texture was not affected by these treatment, in which compact callus texture was observed in all treatments.Keywords: Induction of callus, 2,4-D, BAP, peanuts

Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron

1992 ◽  
Vol 70 (6) ◽  
pp. 1186-1192 ◽  
Author(s):  
R. Gill ◽  
Praveen K. Saxena
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Somatic Embryos ◽  
Direct Somatic Embryogenesis ◽  
Shoot Buds ◽  
Arachis Hypogaea L ◽  
Seedling Explants

An efficient procedure has been developed for inducing direct somatic embryogenesis, organogenesis, and regeneration of plants from tissue cultures of peanut (Arachis hypogaea L.). Thin transverse sections of the cotyledons and juvenile leaves were cultured on Murashige and Skoog medium supplemented with N6-benzylaminopurine (BAP) or a substituted phenylurea, thidiazuron (TDZ). Somatic embryos or shoot buds differentiated from cut surfaces of the cotyledons and midrib region of the leaves. The application of BAP induced differentiation of shoot buds whereas the treatment with TDZ resulted in the production of somatic embryos. Somatic embryos developed into plants after subculturing on a basal meduim. Agar-solidified medium was found to be superior to the liquid medium for the development of embryos and shoot buds. The procedure of TDZ-induced somatic embryogenesis and plant regeneration was successfully applied to three genotypes of peanut. A distinct feature of this study is the induction of the morphogenic competence in cultures of seedling expiants of peanut that so far have remained recalcitrant to somatic embryogenesis in vitro. Key words: peanut, Arachis hypogaea, shoot regeneration, somatic embryogenesis, thidiazuron, plant regeneration.

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