Development of In-Vitro Regeneration Protocol From Seedling Explants of Populus Gamblei Dode: A Difficult to Propagate Endemic Tree Species of Eastern Himalayas

This is the first report of in-vitro regeneration of Populus gamblei, an endemic and rare tree of montane to subtropical forest of Eastern Himalayas. Nodal segment from coppice shoot of young headed back seedling resulted in 100 % sprouting in MS + 1.0 mg L-1 BAP. Each micro shoot proliferated and gave 7.06 micro shoots on MS + 0.50 mg L-1 BAP + 0.10 mg L-1 IBA. In vitro multiplied shoots produced 100 % rooting in ½ MS + 0.5 mg L-1 NAA. In-vitro regenerated plants recorded 80 percent survival during hardening.