Vigorous growth of fusion products allows highly efficient selection of interspecific potato somatic hybrids: molecular proofs

1993 ◽  
Vol 12-12 (7-8) ◽  
Author(s):  
Zs. Polg�r ◽  
J. Preiszner ◽  
D. Dudits ◽  
A. Feh�r
Keyword(s):  
Somatic Hybrids ◽  
Highly Efficient ◽  
Vigorous Growth ◽  
Fusion Products ◽  
Efficient Selection ◽  
Selection Of

scholarly journals Highly efficient selection of epitope specific antibody through competitive yeast display library sorting

mAbs ◽  
2013 ◽  
Vol 5 (4) ◽  
pp. 533-539 ◽  
Author(s):  
Vinita Puri ◽  
Emily Streaker ◽  
Ponraj Prabakaran ◽  
Zhongyu Zhu ◽  
Dimiter S. Dimitrov
Keyword(s):  
Specific Antibody ◽  
Yeast Display ◽  
Highly Efficient ◽  
Efficient Selection ◽  
Selection Of

Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA′ fusions on live bacteria 1 1Edited by J. Wells

2000 ◽  
Vol 301 (4) ◽  
pp. 893-904 ◽  
Author(s):  
Itai Benhar ◽  
Ronit Azriel ◽  
Limor Nahary ◽  
Shelly Shaky ◽  
Yevgeny Berdichevsky ◽  
...  
Keyword(s):  
Highly Efficient ◽  
Phage Antibodies ◽  
Live Bacteria ◽  
Efficient Selection ◽  
Selection Of

scholarly journals PRODUCTION OF SOMATIC HYBRIDS BETWEEN LYCOPERSICON HIRSUTUM AND L. ESCULENTUM.

HortScience ◽  
1991 ◽  
Vol 26 (6) ◽  
pp. 713F-713 ◽  
Author(s):  
T.J. Montagno ◽  
P.S. Jourdan ◽  
S. Z. Berry
Keyword(s):  
Somatic Hybrids ◽  
Leaf Shape ◽  
Fruit Size ◽  
Iodoacetic Acid ◽  
Lycopersicon Hirsutum ◽  
Unilateral Incompatibility ◽  
Hybrid Plants ◽  
Intermediate Morphology ◽  
Fusion Products ◽  
Selection Of

Unilateral incompatibility has limited the direction of crossing between L. esculentum and L. hirsutum; the latter can only serve as the pollen parent. In an attempt to introduce the L. hirsutum cytoplasm into L. esculentum, thirty-three somatic hybrid plants have been regenerated following four separate fusions between leaf protoplasts of L. hirsutum PI 126445 and etiolated hypocotyl protoplasts of L. esculentum (`OH7870', `OH832', and `OH8245'). A 33% PEG solution supplemented with 10% DMSO was used as the fusogen. Selection of fusion products was based on treatment of L. hirsutum protoplasts with 1 mM iodoacetic acid and non-regenerability of the L. esculentum genotypes. Hybridity was initially confirmed by intermediate morphology, including leaf shape, type of trichomes, flower shape, stigma placement, and fruit size and color. Isozyme analysis for GOT, PGM, and 6-PDH verified hybridity. Six of the hybrids produced viable seed upon selfing. At least some of the hybrids contained chloroplast DNA from L. hirsutum, indicating that the wild species cytoplasm may be present in these plants.

scholarly journals A novel sorting technology allows for highly efficient selection of sperm without chromatin damage

2013 ◽  
Vol 59 (3) ◽  
pp. 172-177 ◽  
Author(s):  
Michael G. Funaro ◽  
Howard H. Kim ◽  
Svetlana Mazel ◽  
Alexander Bolyakov ◽  
Marc Goldstein ◽  
...  
Keyword(s):  
Highly Efficient ◽  
Efficient Selection ◽  
Selection Of
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