Characterization of Soluble Sugar Content, Related Enzyme Activity and Gene Expression in the Fruits of ‘Minihyang’ Mandarin on Different Rootstocks

‘Minihyang’ mandarin bears fruits with small size and high sugar content. ‘Minihyang’ mandarin grafted on trifoliate orange (TO) tends to be vigorous and develops water sprout open. It is associated with insufficient floral differentiation and fruit set. Recently, the use of Flying Dragon (FD) as rootstock with a high dwarf effect has been proposed to improve this situation. Therefore, this study was conducted to evaluate the effect of two different rootstock genotypes on tree growth, fruit yield, and fruit quality at the physiological, biochemical, and molecular levels. As a result of the study, in FD, tree vigor was stably maintained, fruit size was large, and the sugar content was high compared to the TO. Fructose, glucose, and sucrose of fruit continued to increase from development to maturity. In particular, fructose and sucrose were significantly higher in the fruits of the FD than those in TO at 150 and 220 days after anthesis. The total sugar content was also significantly higher in the fruit of the FD. The activities of SPS and SS associated with sucrose synthesis tended to be increased during the fruit maturity season, but there was no significant difference between the two rootstocks. On the other hand, the activities of SS and AI breaking down sucrose were high in FD at 150 and 220 days after anthesis. These results suggest that the unloading of sucrose might be increased and affect the sugar content. However, the results of real-time PCR analysis of gene expression related to sucrose metabolism did not show an association with changes in enzyme activity affecting sugar content. Therefore, further detailed studies on the process after the regulation of gene expression are likely to be needed.

Genome-wide identification and expression profile of YABBY genes in Averrhoa carambola

Background Members of the plant-specific YABBY gene family are thought to play an important role in the development of leaf, flower, and fruit. The YABBY genes have been characterized and regarded as vital contributors to fruit development in Arabidopsis thaliana and tomato, in contrast to that in the important tropical economic fruit star fruit (Averrhoa carambola), even though its genome is available. Methods In the present study, a total of eight YABBY family genes (named from AcYABBY1 to AcYABBY8) were identified from the genome of star fruit, and their phylogenetic relationships, functional domains and motif compositions, physicochemical properties, chromosome locations, gene structures, protomer elements, collinear analysis, selective pressure, and expression profiles were further analyzed. Results Eight AcYABBY genes (AcYABBYs) were clustered into five clades and were distributed on five chromosomes, and all of them had undergone negative selection. Tandem and fragment duplications rather than WGD contributed to YABBY gene number in the star fruit. Expression profiles of AcYABBYs from different organs and developmental stages of fleshy fruit indicated that AcYABBY4 may play a specific role in regulating fruit size. These results emphasize the need for further studies on the functions of AcYABBYs in fruit development.

Understanding the fruit sink.

This chapter deals with understanding the fruit sink by studying the floral development and structures; pollination, fertilization, fruit set and types; limitations of fruit growth; thinning effects on fruit growth, size and yield; spring temperature effects on fruit size; nut development and growth; and the condition of alternate bearing.

Pest Infestation of Dipterocarpus retusus (Blume) Fruit at Different Heights in KPHP BATULANTEH

Keruing gunung (Dipterocarpus retusus) is a non-timber forest product (NTFP) as a fruit producer that can be used as raw material for vegetable fats. One of the problems faced in planting programs for both production and conservation forests is the presence of fruit pests. The purpose of this study was to determine the fruit pests infestation fruits of D. retusus and the effect of altitude on fruit size and weight in Batulanteh Sumbawa. Fruit samples were collected at locations with different heights, namely below 1000 masl (T 22ºC, RH 83%) and above 1000 masl (T 20ºC, RH 88%). The samples of invading pests were observed and measured morphometry and morphology. The results showed that the insect infestation on the fruit was Alcidodes crassus. The percentage of fruit severity level reached more than 50% at each location. Elevation has a significant effect on fruit diameter and fruit weight, both infested by pests and whole fruit.

Effects of Foliar Applications of Some Macro and Micro Nutrients on Yield, Quality Attributes of Guava (Psidium Guajava L.)

Effects of foliar applied macro (NPK) and micro nutrients (Fe, Mn, Zn, Cu and B) at different time on yield, quality and benefit: cost ratio of rainy and winter season guava were evaluated in north western Haryana, India. The two season’s data depicted that physico- chemical characters with respect to fruit set, fruit retention, fruit size, average fruit weight and yield were significantly increased with foliar application of macro and micro nutrients at vegetative, flowering and fruiting stage (T7). The shoot C: N ratio was also found highest with T7 foliar treatment which was followed by T5 in both the seasons. The T7 foliar spray resulted in maximum benefit: cost ratio in both the seasons. Proper fertilization strategies can help fruit growers to get good yield and quality fruits. Bangladesh J. Bot. 50(4): 1159-1164, 2021 (December)

Comparative study on the phenology, gender and yield components of cucumber (Cucumis sativus L.) varieties

The intensity of sex expression is important in crops, including cucumber, since sex form and flowering have direct effects on date of harvesting and yield. Two groups of cucumber comprising a native variety (Odukpani) and elite varieties (Griffaton, Poinsett, Ashley, Marketmore and Monarch) were evaluated in 2015 and 2016 cropping seasons at the Teaching and Research Farm, College of Agriculture, Garkawa, Plateau State; to assess flowering, sex expression and some yield traits. The experimental design was randomized complete block design with the 6 cucumber varieties as the treatments, replicated five times. The two groups differed significantly (p < 0.05) in phonological, flowering and yield traits assessed. The elite varieties were superior with respect to flowering traits, as they flowered early. The intra-population hierarchical cluster analysis of quantitative traits grouped the elite varieties in one main cluster and the native variety (Odukpani) alienated as an outlier. The cluster plots showed that Odukpani was robust in vegetative growth, produced few but larger fruit size and flowering was delayed for over two weeks. Conversely, the elite varieties flowered early, produced more flowers and recorded superior number of fruits per plant. The profuse production of pistillate flowers by the elite varieties had pronounced yield advantage over the local variety. Hybridization between the two groups of cucumber would make a mark in the drive for sustainable cucumber fruit yield.

Designing the best breeding strategy for Coffea canephora: Genetic evaluation of pure and hybrid individuals aiming to select for productivity and disease resistance traits

Breeding programs of the species Coffea canephora rely heavily on the significant genetic variability between and within its two varietal groups (conilon and robusta). The use of hybrid families and individuals has been less common. The objectives of this study were to evaluate parents and families from the populations of conilon, robusta, and its hybrids and to define the best breeding and selection strategies for productivity and disease resistance traits. As such, 71 conilon clones, 56 robusta clones, and 20 hybrid families were evaluated over several years for the following traits: vegetative vigor, incidence of rust and cercosporiosis, fruit ripening time, fruit size, plant height, canopy diameter, and yield per plant. Components of variance and genetic parameters were estimated via residual maximum likelihood (REML) and genotypic values were predicted via best linear unbiased prediction (BLUP). Genetic variability among parents (clones) and hybrid families was detected for most of the evaluated traits. The Mulamba-Rank index suggests potential gains up to 17% for the genotypic aggregate of traits in the hybrid population. An intrapopulation recurrent selection within the hybrid population would be the best breeding strategy because the genetic variability, narrow and broad senses heritabilities and selective accuracies for important traits were maximized in the crossed population. Besides, such strategy is simple, low cost and quicker than the concurrent reciprocal recurrent selection in the two parental populations, and this maximizes the genetic gain for unit of time.

Wi-Fruit

People usually assess fruit qualities from external features such as color, shape, size, and texture. However, it is quite common that we select fruits with perfect appearances but rotten inside, especially for fruits with thick pericarps. Thus the accurate measurement is desirable to evaluate the internal conditions of fruits. As two key features of fruit internal qualities, existing methods on measuring fruit moisture and soluble solid contents (SSC) are either destructive or costly, limiting their adoption in daily life. In this paper, we propose Wi-Fruit, a non-destructive and low-cost fruit moisture and SSC measurement system leveraging Wi-Fi channel state information (CSI). First, to cope with the fruit structure dependency challenge, we propose a double-quotient model to pre-process CSI on adjacent antennas. Second, to address the fruit size and type dependency challenges, a lightweight artificial neural network (ANN) model with visual information fusion is proposed for fruit moisture and SSC estimations. Extensive evaluations are conducted on 6 types of fruits with both thick (i.e., watermelon and grapefruit) and thin pericarps (i.e., dragon fruit, apple, pear, and orange) over a month in either an empty laboratory room or a library with massive books. Results demonstrate that Wi-Fruit achieves an acceptable estimation accuracy (RMSE=0.319). It is independent of various fruit structures, sizes, and types, while also robust to time and environmental changes. The fruit internal sensing capabilities of Wi-Fruit can help fruit saving and safety in both pre-harvest and post-harvest applications.