Osmotically-regulated trehalose accumulation and cyclic β-(1,2)-glucan excretion by Rhizobium leguminosarum biovar trifolii TA-1

1991 ◽  
Vol 156 (6) ◽  
pp. 501-506 ◽  
Author(s):  
Michaël W. Breedveld ◽  
Ludovicus P. T. M. Zevenhuizen ◽  
Alexander J. B. Zehnder
Keyword(s):  
Rhizobium Leguminosarum ◽  
Rhizobium Leguminosarum Biovar Trifolii ◽  
Trehalose Accumulation

Slow rehydration improves the recovery of dried bacterial populations

1992 ◽  
Vol 38 (6) ◽  
pp. 520-525 ◽  
Author(s):  
J. W. Kosanke ◽  
R. M. Osburn ◽  
G. I. Shuppe ◽  
R. S. Smith
Keyword(s):  
Relative Humidity ◽  
Rhizobium Leguminosarum ◽  
Moisture Absorption ◽  
Rhizobium Meliloti ◽  
Bacterial Populations ◽  
Bulk Powder ◽  
Alfalfa Seed ◽  
Powder Samples ◽  
Cellular Stresses ◽  
Rhizobium Leguminosarum Biovar Trifolii

Slow rehydration of bacteria from dried inoculant formulations provided higher viable counts than did rapid rehydration. Estimates were higher when clay and peat powder formulations of Rhizobium meliloti, Rhizobium leguminosarum biovar trifolii, and Pseudomonas putida, with water activities between 0.280 and 0.650, were slowly rehydrated to water activities of approximately 0.992 before continuing the dilution plating sequence. Rhizobium meliloti populations averaged 6.8 × 108 cfu/g and 1328 cfu/alfalfa seed greater when slowly rehydrated from bulk powder and preinoculated seeds, respectively. Bulk powder samples were slowly rehydrated to 0.992 water activity by the gradual addition of diluent, followed by a 10-min period for moisture equilibration. Preinoculated seed samples were placed in an environmental chamber at 24 °C with relative humidity greater than 80% for 1 h to allow moisture absorption. "Upshock," osmotic cellular stresses that occur during rehydration, was reduced when dried microbial formulations were slowly rehydrated and equilibrated before becoming fully hydrated in the dilution plating sequence. These procedures may also be applicable when estimating total viable bacterial populations from dried soil or other dry formulations. Key words: rehydration procedure, microbial rehydration, desiccation, Rhizobium, Pseudomonas.

scholarly journals Competitiveness of a native Rhizobium leguminosarum biovar trifolii strain for nodule occupancy is manifested during infection

2008 ◽  
Vol 318 (1-2) ◽  
pp. 117-126 ◽  
Author(s):  
Samuel Duodu ◽  
Caroline Brophy ◽  
John Connolly ◽  
Mette M. Svenning
Keyword(s):  
Rhizobium Leguminosarum ◽  
Nodule Occupancy ◽  
Rhizobium Leguminosarum Biovar Trifolii

Structurally diverse chitolipooligosaccharide Nod factors accumulate primarily in membranes of wild-type Rhizobium leguminosarum biovar trifolii

Biochemistry ◽  
1995 ◽  
Vol 34 (11) ◽  
pp. 3832-3840 ◽  
Author(s):  
Guy G. Orgambide ◽  
Jeongrim Li ◽  
Rawle I. Hollingsworth ◽  
Frank B. Dazzo
Keyword(s):  
Rhizobium Leguminosarum ◽  
Nod Factors ◽  
Wild Type ◽  
Rhizobium Leguminosarum Biovar Trifolii

scholarly journals The Rhizobium leguminosarum biovar viciae nodO gene can enable a nodE mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch

Microbiology ◽  
1994 ◽  
Vol 140 (9) ◽  
pp. 2341-2347 ◽  
Author(s):  
A. Economou ◽  
A. E. Davies ◽  
A. W. B. Johnston ◽  
J. A. Downie
Keyword(s):  
Rhizobium Leguminosarum ◽  
Rhizobium Leguminosarum Biovar Trifolii
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