Regeneration of leaf mesophyll protoplasts of tomato cultivars (L. esculentum): factors important for efficient protoplast culture and plant regeneration

1987 ◽  
Vol 6 (3) ◽  
pp. 172-175 ◽  
Author(s):  
Mei-Lie M. C. Tan ◽  
Ellen M. Rietveld ◽  
Gijsbert A. M. van Marrewijk ◽  
Ad J. Kool
Keyword(s):  
Plant Regeneration ◽  
Protoplast Culture ◽  
Mesophyll Protoplasts ◽  
Leaf Mesophyll ◽  
Tomato Cultivars

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

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