scholarly journals Autotetraploid plant regeneration by indirect somatic embryogenesis from leaf mesophyll protoplasts of diploid Gentiana decumbens L.f.

2015 ◽  
Vol 51 (3) ◽  
pp. 350-359 ◽  
Author(s):  
Karolina Tomiczak ◽  
Anna Mikuła ◽  
Elwira Sliwinska ◽  
Jan J. Rybczyński
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Mesophyll Protoplasts ◽  
Leaf Mesophyll ◽  
Indirect Somatic Embryogenesis

scholarly journals Plant Regeneration from Leaf Mesophyll Protoplasts of Lactuca sativa L.

1986 ◽  
Vol 3 (2) ◽  
pp. 83-85 ◽  
Author(s):  
Hiroshi SUZUKI ◽  
Sueo ENOMOTO ◽  
Katsuo OHYAMA
Keyword(s):  
Plant Regeneration ◽  
Lactuca Sativa ◽  
Mesophyll Protoplasts ◽  
Leaf Mesophyll ◽  
Lactuca Sativa L

scholarly journals Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 768
Author(s):  
Yue Fei ◽  
Lan-Xiang Wang ◽  
Zheng-Wu Fang ◽  
Zhi-Xiong Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Fagopyrum Esculentum ◽  
Cereal Crop ◽  
Embryogenic Calli ◽  
Hypocotyl Explants ◽  
Indirect Somatic Embryogenesis ◽  
Fagopyrum Esculentum Moench

Buckwheat (Fagopyrum esculentum, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of F. esculentum. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL−1 2,4-D and 1.5 mgL−1 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL−1 6-BA and 0.5 mgL−1 thidiazuron (TDZ)two to three times subculture at 40–60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL−1 6-BA and 0.5 mgL−1 TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of F. esculentum.

scholarly journals Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

2011 ◽  
Vol 02 (02) ◽  
pp. 262-267 ◽  
Author(s):  
Aung Htay Naing ◽  
Jae Dong Chung ◽  
Ki Byung Lim
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Indirect Somatic Embryogenesis
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