Cellulolytic complex of Aspergillus niger under conditions for citric acid production. Isolation and characterization of two ?-(1?4)-glucan hydrolases

1984 ◽  
Vol 20 (5) ◽  
pp. 326-330 ◽  
Author(s):  
Smilja Vidmar ◽  
Vito Turk ◽  
Igor Kregar
1985 ◽  
Vol 31 (5) ◽  
pp. 436-440 ◽  
Author(s):  
David W. Good ◽  
Randal Droniuk ◽  
G. Ross Lawford ◽  
Jared E. Fein

A process for the production of citric acid from canola (rapeseed) oil using the yeast Saccharomycopsis lipolytica was examined. A citrate nonutilizing strain, designated NTG9, which had an improved citric to isocitric acid ratio, was isolated after mutagenesis of S. lipolytica ATCC 20228 with nitrosoguanidine. Although the mutant grew well on canola oil, unlike the parent strain or a spontaneous revertant (JF2), it did not grow on glycolytic intermediates below glycerate-3-phosphate, amino acids, hexadecane, or yellow kerosene. The mutant was shown to be impaired in the gluconeogenic pathway because of a loss of phosphoenolpyruvate carboxykinase activity. A preliminary study of the effect of micronutrients on citric acid production by S. lipolytica NTG9 showed that manganese had a stimulatory effect on the process whereas zinc and iron were inhibitory. A revised growth medium was tested and found to increase citric acid production while decreasing that of isocitric acid.


1963 ◽  
Vol 30 (3) ◽  
pp. 365-379 ◽  
Author(s):  
N. F. MILLIS ◽  
B. H. TRUMPY ◽  
B. M. PALMER

2007 ◽  
Vol 1 (3) ◽  
pp. 246-253 ◽  
Author(s):  
A. A. Guilherme ◽  
G. A. S. Pinto ◽  
S. Rodrigues

1966 ◽  
Vol 12 (5) ◽  
pp. 901-907 ◽  
Author(s):  
H. Horitsu ◽  
D. S. Clark

Ferrocyanide at concentrations of less than 30 p.p.m. (the amount tolerated in citric acid fermentation of beet molasses) had no measurable effect on citric acid production or on the oxidation of glucose or Krebs cycle compounds by resting cells of Aspergillus niger or on the growth rate of this organism during submerged fermentation of beet molasses. Concentrations above 30 p.p.m., however, stimulated citric acid formation in resting cells, but markedly inhibited cell development in growing cells. This inhibition of growth was the main cause of the detrimental effect of high concentrations of ferrocyanide on citric acid formation in molasses; good growth throughout the fermentation was essential to high acid yield, inhibition of growth could be released at any time during the fermentation by addition of sufficient ZnSO4 to reduce the ferrocyanide content to below 30 p.p.m. No evidence that ferrocyanide favors citric acid accumulation by blocking a reaction in the Krebs cycle was found.


2018 ◽  
Vol 41 (7) ◽  
pp. 1029-1038 ◽  
Author(s):  
Xiaowen Sun ◽  
Hefang Wu ◽  
Genhai Zhao ◽  
Zhemin Li ◽  
Xihua Wu ◽  
...  

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