OPTIMIZATION OF BACTERIAL CELLULOSE PRODUCTION FROM SUGAR BEET MOLASSES BY GLUCONACETOBACTER XYLINUS NRRL B-759 IN STATIC CULTURE

We investigated the optimization of bacterial cellulose (BC) production from sugar beet molasses by Gluconacetobacter xylinus NRRL B-759 in static culture. The optimization studies were performed using the central composite design (CCD) of response surface methodology (RSM). The independent variables were the molasses concentration, inoculation ratio and culture volume. The dependent variable was BC production yield. From the optimization tests, based on the model developed by RSM-ANOVA, it was found that binary interactions between molasses concentration–culture volume and inoculation ratio–culture volume had the most significant influence on the responses. The optimum conditions were as follows: 78.932 g/L molasses concentration, 12.973% inoculation ratio, and 130.405 mL of culture volume. The obtained BC was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and elemental analysis. The characterization results obtained in the study revealed that the produced BC exhibited typical FTIR spectrum, elemental composition, and nanofiber structure.

Reaction Mechanism of the Microwave-Assisted Synthesis of 5-Hydroxymethylfurfural from Sucrose in Sugar Beet Molasses

5-hydroxymethylfurfural (HMF) stands out among the chemical products derived from biomass as a building block in the chemical industry. The conventional production of HMF is usually carried out from fructose, glucose, or other monosaccharides as feedstock, but sugar beet molasses, a by-product of the sugar industry containing sucrose (45–55%), is promising. This exploratory study used three aqueous stock solutions and one biphasic system as the sources of sucrose. The dehydration of sucrose to 5-hydroxymethylfurfural was assisted by microwave heating and subcritical water conditions. The maximum yield of HMF was 27.8 mol % for the aqueous solution of synthetic sucrose at 80 min of treatment. Although HMF yield was 7.1 mol % in the aqueous sugar beet molasses solution, it increased 2-fold after clarification (15.1 mol %) and 1.6-fold in the biphasic system (11.4 mol %). These are favorable outcomes since this is an exploratory investigation. The pseudo-first-order model fitted experimental data from the conversion of the sucrose from the stock solutions, and kinetic parameters were estimated and compared. The estimated reaction rate constant showed that inversion of sucrose is faster than fructose dehydration to HMF, but the latter reaction was the rate-determining step only for the biphasic system. The maximum partition coefficient value was four between 40 min and 60 min of reaction, calculated at room temperature. These predictions help investigators to estimate conversions and selectivity when pilot plants need to be simulated.

Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources

Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from B. paramycoides and A. salinestris showed similar bands which confirmed the presence of PHB; however, B. naejangsanensis showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for B. paramycoides and isopropyl ester of 2-butenoic acid for both of A. salinestris and Brevundimonas naejangsanensis. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.

Beet Molasses Enhance Salinity Tolerance in Thymus serpyllum—A Study under Greenhouse Condition

The growing demand for Thymus serpyllum biomass to produce drugs, cosmetics and spices necessitates the search for innovative methods mitigating the negative effects of environmental stressors in order to improve its yield under unfavorable conditions. Due to the exposure of plants to salinity stress (SS), we investigated the effect of sugar beet molasses (SBM) on the growth and biochemical parameters related to plants’ response to SS. Wild thyme plants were treated for 5 weeks to sodium chloride and 3% molasses solution using two modes of application (soil irrigation or foliar sprays). Plants irrigated by SBM showed slighter stem growth inhibition than control plants, high stress tolerance index and maintained a constant root water content under salt stress. Moreover plants treated with 100 mM NaCl and soil-applied SBM had lower lipid peroxidation level, showed lower POD activity, higher total soluble protein content and maintained a more even free amino acids level, compared to the control treatments. The concentration of potassium ions was higher in the case of plant roots irrigation with sugar beet molasses compared to control plants. In this experiment, most of the growth and biochemical parameters from foliar molasses-sprayed plants did not differ significantly from the control. We provided evidence that soil-applied SBM beneficially changed the plant’s biochemical response to salt stress. On the basis of the obtained results, we conclude that this soil amendment contributes to the strengthening of plant protection against this harmful environmental factor.