Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication

1981 ◽  
Vol 184 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Janice Light ◽  
Søren Molin
1983 ◽  
Vol 192 (1-2) ◽  
pp. 140-148 ◽  
Author(s):  
Michael B. Yarmolinsky ◽  
Evelyn Stevens

1979 ◽  
Vol 43 (0) ◽  
pp. 419-425 ◽  
Author(s):  
P. Gustafsson ◽  
H. Dreisig ◽  
S. Molin ◽  
K. Nordstrom ◽  
B. E. Uhlin

1988 ◽  
Vol 7 (5-6) ◽  
pp. 559-564
Author(s):  
E. G. H. Wagner ◽  
C. Persson ◽  
M. Öhman ◽  
K. Nordstrom

2004 ◽  
Vol 186 (1) ◽  
pp. 207-211 ◽  
Author(s):  
Jan A. Olsson ◽  
Johan Paulsson ◽  
Kurt Nordström

ABSTRACT Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter at low copy numbers, leading to increased repA mRNA synthesis. This promoter is normally switched off by a constitutively synthesized plasmid-encoded repressor protein, CopB; in cells with low copy numbers, the concentration of CopB is low and the promoter is derepressed. Here we show that the rate of loss of a Par+ derivative of the basic replicon of R1 increased about sevenfold when the cells contained a high concentration of the CopB protein formed from a compatible plasmid.


1995 ◽  
Vol 17 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Rasmus Bugge Jensen ◽  
Elisabeth Grohmann ◽  
Helmut Schwab ◽  
Ramón Díaz-Orejas ◽  
Kenn Gerdes

1986 ◽  
Vol 14 (13) ◽  
pp. 5574-5574
Author(s):  
E. Gerhart ◽  
H. Wagner ◽  
Kurt Nordström

1994 ◽  
Vol 12 (1) ◽  
pp. 49-60 ◽  
Author(s):  
Pontus Blomberg ◽  
Hilde M. Engdahl ◽  
Charlotta Malmgren ◽  
Pascale Romby ◽  
E. Gerhart H. Wagner

Nature ◽  
1976 ◽  
Vol 262 (5569) ◽  
pp. 623-626 ◽  
Author(s):  
RICHARD P. NOVICK ◽  
MARJORIE SCHWESINGER

Sign in / Sign up

Export Citation Format

Share Document