Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae

1988 ◽  
Vol 7 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Jan Fahleson ◽  
Johan Dixelius ◽  
Eva Sundberg ◽  
Kristina Glimelius
Keyword(s):  
Chromosome Number ◽  
Dna Content ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Nuclear Dna Content ◽  
Flow Cytometric

scholarly journals Electrofusion of protoplasts of anther-derived dihaploid lines of commercial potato cultivars

1996 ◽  
Vol 5 (4) ◽  
pp. 449-460 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Yong-Sheng Xu ◽  
Pirjo Tanhuanpää ◽  
Leena Pietilä ◽  
Eija Pehu
Keyword(s):  
Dna Content ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Solanum Tuberosum L ◽  
Rapd Marker ◽  
Nuclear Dna Content ◽  
Parental Line ◽  
Flow Cytometric ◽  
Commercial Potato

Somatic hybrids of anther-derived dihaploid (2x) potato (Solanum tuberosum L.) lines were produced by electrofusion of protoplasts. Using RAPD (randomly amplified polymorphic DNA) markers, six new combinations of dihaploid parental lines from cultivars Matilda, Nicola, Pito, Stina, Van Gogh and White Lady were identified. RAPD marker identification of the putative hybrids was mostly done using two distinct parental line specific primers. 43% of the 76 regenerated calli from the six combinations produced hybrid shoots. Most of the somatic hybrids were tetraploid (4x), but in four fusion combinations plants of hexaploid (6x), octoploid (8x) or mixoploid level were also identified by chromosome counts or flow cytometric nuclear DNA analysis. The mean nuclear DNA content (2C value) of the tetraploid and hexaploid somatic hybrids was lower than the expected DNA content (i.e. the 2C values of the original tetraploid cultivars or the sum of the 2C values of the dihaploid fusion parents). Some somatic hybrids having the expected nuclear DNA content were also found.

Flow cytometric determination of nuclear DNA content in benign adrenal pheochromocytomas

Urology ◽  
1987 ◽  
Vol 30 (2) ◽  
pp. 102-104 ◽  
Author(s):  
James B. Amberson ◽  
E. Darracott Vaughan ◽  
George F. Gray ◽  
Gregory J. Naus
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Flow Cytometric

scholarly journals Determination of Nuclear DNA Content and Ploidy Level in Rubus by Flow Cytometry

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 514D-514
Author(s):  
Rengong Meng ◽  
Chad E. Finn ◽  
Robert P. Doss
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dna Flow Cytometry ◽  
Chromosome Counting ◽  
Mature Leaves ◽  
Microscopic Evaluation

Knowledge of the chromosome number in Rubus would be valuable when planning crosses and identifying plants, etc., however, preparation of tissue for microscopic evaluation and chromosome counting is difficult and time-consuming. Flow cytometry offers a more-efficient approach to this task. DNA flow cytometry was used to determine the nuclear DNA content in 22 Rubus genotypes. The genotypes represented a range of reported chromosome numbers from 2x to 12x. Six of the genotypes were representatives of Rubus ursinus, which is reported to have both 8x and 12x forms. Samples of nuclei were prepared from leaf discs of newly emerged and mature leaves following published protocols with some modifications. The DNA content was estimated by comparison of the fluorescence of Rubus nuclei with an internal DNA standard. There was an increase in nuclear DNA content concurrent with the increase in chromosome number. In these studies DNA flow cytometry could differentiate genotypes that differed by 2x, such as 6x and 8x, but could not reliably distinguish genotypes that differed by 1x, such as 7x vs. 8x or 6x. Aneuploids cannot be differentiated at this time.

Prognostic Implications of Nuclear DNA Content in Head and Neck Cancer

1989 ◽  
Vol 100 (2) ◽  
pp. 95-98 ◽  
Author(s):  
Yang-Chun Guo ◽  
Lawrence Desanto ◽  
Gregory V. Osetinsky
Keyword(s):  
Head And Neck ◽  
Dna Content ◽  
Nuclear Dna ◽  
Clinical Stage ◽  
Nuclear Dna Content ◽  
Survival Advantage ◽  
Prognostic Information ◽  
Diagnosis Of Cancer ◽  
Well Differentiated

The nuclear DNA content was measured in formalin-fixed and deparaffined specimens of 296 oral, pharyngeal, and laryngeal squamous cell carcinomas from patients in whom the clinical outcome was known. One hundred ninety (64%) contained cells with abnormal DNA (DNA aneuploid or tetra/polypoid). Only 32% (60 of 190) of the patients with DNA nondiploid cancers survived 5 years, compared with 49% (52 of 106) of the patients with DNA diploid cancers. When the findings were controlled for clinical stage, patients whose tumors were DNA diploid had a survival advantage at each stage. Histologic grading showed less correlation, because only patients with well-differentiated carcinomas had a survival advantage if their tumors were DNA diploid. These data showed that determination of DNA content in cancers of the head and neck can offer prognostic information not provided by other means and enhance the diagnosis of cancer.

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