Physarum polycephalum: Establishing an Assay for Testing Decision-making Under Shifting Somatic Boundaries

Prior studies of decision-making generally assume a fixed agent which maximizes utility among its various options. Physarum polycephalum is a popular model for basal cognition that can be cut into pieces that may or may not re-join. We exploited this capacity to develop a novel assay in which radical changes to the agent itself are among the options of the decision-making process. Specifically, we transected a Physarum culture in the presence of a food reward that was located closer to the new smaller piece. In this scenario, the newly created branch must choose between exploiting the reward itself, or first re-connecting with the original mass (and sharing the nutrient reward across a large body). We report a pilot study establishing a protocol in which the number of agents is part of the decision-making process. We observed that despite the presence of food, new branches strongly prefer to merge back to the syncytium before exploiting the reward. Many improvements to the protocol are possible, to extend this effort to understand the interplay between behavioral options and the structure and boundary of the individual making choices in its environment

Identification and characterization of histones in Physarum polycephalum evidence a phylogenetic vicinity of Mycetozoans to the animal kingdom

Physarum polycephalum belongs to Mycetozoans, a phylogenetic clade apart from the animal, plant and fungus kingdoms. Histones are nuclear proteins involved in genome organization and regulation and are among the most evolutionary conserved proteins within eukaryotes. Therefore, this raises the question of their conservation in Physarum and the position of this organism within the eukaryotic phylogenic tree based on histone sequences. We carried out a comprehensive study of histones in Physarum polycephalum using genomic, transcriptomic and molecular data. Our results allowed to identify the different isoforms of the core histones H2A, H2B, H3 and H4 which exhibit strong conservation of amino acid residues previously identified as subject to post-translational modifications. Furthermore, we also identified the linker histone H1, the most divergent histone, and characterized a large number of its PTMs by mass spectrometry. We also performed an in-depth investigation of histone genes and transcript structures. Histone proteins are highly conserved in Physarum and their characterization will contribute to a better understanding of the polyphyletic Mycetozoan group. Our data reinforce that P. polycephalum is evolutionary closer to animals than plants and located at the crown of the eukaryotic tree. Our study provides new insights in the evolutionary history of Physarum and eukaryote lineages.

Physarum-Inspired Bicycle Lane Network Design in a Congested Megacity

Improvement of mobility, especially environment-friendly green mobility, is challenging in existing megacities due to road network complexity and space constraints. Endorsing the bicycle lane network (BLN) in congested megacities is a promising option to foster green mobility. This research presents a novel bioinspired network design method that considers various constraints and preferences related to the megacity for designing an optimal BLN. The proposed method is inspired by natural Physarum polycephalum, a brainless, multi-headed single-celled organism, which is capable of developing a reticulated network of complex foraging behaviors in pursuit of food. The mathematical model of Physarum foraging behavior is adapted to maneuver various BLN constraints in megacity contexts in designing the optimal BLN. The Physarum-inspired BLN method is applied to two case studies on the megacity Dhaka for designing BLNs: the first one covers congested central city area, and the second one covers a broader area that includes major locations of the city. The obtained BLNs were evaluated comparing their available routes between different locations with the existing vehicle routes of the city in terms of distance and required travel times in different time periods, and the BLN routes were found to be suitable alternatives for avoiding congested main roads. The expected travel time using BLNs is shorter than other transport (e.g., car and public bus); additionally, at glance, the average travel speed on BLNs is almost double that of public buses in peak hours. Finally, the designed BLNs are promising for environment-friendly and healthy mobility.

Swarm Robot Exploration Strategy for Path Formation Tasks Inspired by Physarum polycephalum

Physarum polycephalum, a unicellular and multiheaded slime mould, can form highly efficient networks connecting separated food sources during the process of foraging. These adaptive networks exhibit a unique characteristic in that they are optimized without the control of a central consciousness. Inspired by this phenomenon, we present an efficient exploration and navigation strategy for a swarm of robots, which exploits cooperation and self-organisation to overcome the limited abilities of the individual robots. The task faced by the robots consists in the exploration of an unknown environment in order to find a path between two distant target areas. For the proposed algorithm (EAIPP), we experimentally present robustness tests and obstacle tests conducted to analyse the performance of our algorithm and compare the proposed algorithm with other swarm robot foraging algorithms that also focus on the path formation task. This work has certain significance for the research of swarm robots and Physarum polycephalum. For the research of swarm robotics, our algorithm not only can lead multirobot as a whole to overcome the limitations of very simple individual agents but also can offer better performance in terms of search efficiency and success rate. For the research of Physarum polycephalum, this work is the first one combining swarm robots and Physarum polycephalum. It also reveals the potential of the Physarum polycephalum foraging principle in multirobot systems.