scholarly journals Electrofusion of protoplasts of anther-derived dihaploid lines of commercial potato cultivars

1996 ◽  
Vol 5 (4) ◽  
pp. 449-460 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Yong-Sheng Xu ◽  
Pirjo Tanhuanpää ◽  
Leena Pietilä ◽  
Eija Pehu
Keyword(s):  
Dna Content ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Solanum Tuberosum L ◽  
Rapd Marker ◽  
Nuclear Dna Content ◽  
Parental Line ◽  
Flow Cytometric ◽  
Commercial Potato

Somatic hybrids of anther-derived dihaploid (2x) potato (Solanum tuberosum L.) lines were produced by electrofusion of protoplasts. Using RAPD (randomly amplified polymorphic DNA) markers, six new combinations of dihaploid parental lines from cultivars Matilda, Nicola, Pito, Stina, Van Gogh and White Lady were identified. RAPD marker identification of the putative hybrids was mostly done using two distinct parental line specific primers. 43% of the 76 regenerated calli from the six combinations produced hybrid shoots. Most of the somatic hybrids were tetraploid (4x), but in four fusion combinations plants of hexaploid (6x), octoploid (8x) or mixoploid level were also identified by chromosome counts or flow cytometric nuclear DNA analysis. The mean nuclear DNA content (2C value) of the tetraploid and hexaploid somatic hybrids was lower than the expected DNA content (i.e. the 2C values of the original tetraploid cultivars or the sum of the 2C values of the dihaploid fusion parents). Some somatic hybrids having the expected nuclear DNA content were also found.

Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae

1988 ◽  
Vol 7 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Jan Fahleson ◽  
Johan Dixelius ◽  
Eva Sundberg ◽  
Kristina Glimelius
Keyword(s):  
Chromosome Number ◽  
Dna Content ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Nuclear Dna Content ◽  
Flow Cytometric

Flow cytometric measurement of nuclear DNA content variations as a potentialin vivo mutagenicity test

Cytometry ◽  
1981 ◽  
Vol 2 (3) ◽  
pp. 189-191 ◽  
Author(s):  
F. J. Otto ◽  
H. Oldiges ◽  
W. Göhde ◽  
V. K. Jain
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Mutagenicity Test ◽  
Flow Cytometric ◽  
Flow Cytometric Measurement

scholarly journals Reliable flow cytometric estimation of nuclear DNA content in coffee trees

Cytometry ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Philippe Barre ◽  
Michel Noirot ◽  
Jacques Louarn ◽  
Christophe Duperray ◽  
Serge Hamon
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Flow Cytometric

Flow cytometric analysis of nuclear DNA content in Musa

1999 ◽  
Vol 98 (8) ◽  
pp. 1344-1350 ◽  
Author(s):  
M. A. Lysák ◽  
M. Dolez˘elová ◽  
J. P. Horry ◽  
R. Swennen ◽  
J. Dolez˘el
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Flow Cytometric

Cell cycle and germination of fresh, dried and deteriorated sugarbeet seeds as indicators of optimal harvest time

2003 ◽  
Vol 13 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Elwira Śliwińska
Keyword(s):  
Dna Content ◽  
Seed Quality ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Harvest Time ◽  
Flow Cytometric ◽  
Commercial Harvest ◽  
Optimal Harvest ◽  
The Status ◽  
Controlled Deterioration

AbstractSeeds of sugar beet (Beta vulgarisL.) were collected at weekly intervals from 3 weeks before to 1 week after commercial harvest time, dried and stored at room temperature (18–22°C). Laboratory germination tests and flow cytometric analyses were performed immediately after harvest (fresh seeds) and five times at weekly intervals during storage (dry seeds). After 6 months of storage, seeds were exposed to a controlled deterioration treatment (CD). The proportion of G2nuclei in the embryo was constant in the fresh seeds, regardless of their maturity. It decreased, however, after drying and CD, especially in those seeds harvested before maturation drying had commenced. The proportion of endosperm cells in the seed decreased with maturation, and a further decrease was observed after drying and CD. These observations suggest that nuclei with a higher nuclear DNA content were more sensitive to water stress caused by premature desiccation and to deterioration than nuclei with a lower DNA content. Fresh seeds exhibited some germination, but this increased after drying, suggesting that desiccation induced a switch from the developmental to the germination mode. Germination percentages were the highest in dry seeds collected at the commercial harvest time and a week after. This high germinability coincided with the highest proportion of G2cells in the embryo. It is concluded that flow cytometry provides information about the status of sugarbeet seed maturation, seed quality and storage potential, and can be used for estimation of optimal harvest time.

Flow cytometric analysis of nuclear DNA content in oral leukoplakia: relation to clinicopathologic findings

1995 ◽  
Vol 24 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Tohru Saito ◽  
Tomomi Yamashita ◽  
Ken-ichi Notani ◽  
Hiroshi Fukuda ◽  
Shigetaka Mizuno ◽  
...  
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Oral Leukoplakia ◽  
Flow Cytometric ◽  
Clinicopathologic Findings
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