The transposable element Tam1 from Antirrhinum majus shows structural homology to the maize transposon En/Spm and has no sequence specificity of insertion

Abstract The transposable element, Tam3, gives rise to large-scale (greater than 1 kb) chromosomal rearrangements at a low frequency, when it is inserted at the nivea locus of Antirrhinum majus. Although some deletions may result from imprecise excision of Tam3, rearrangements involving deletion, dispersion and inverted duplication of flanking sequences, where Tam3 remains in situ, have also been identified. These rearrangements have been mapped at the molecular level, and the behavior of Tam3 following rearrangement has been observed. It is clear that Tam3 has enormous potential to restructure chromosomes through successive rounds of large-scale rearrangements. The mechanisms by which such rearrangements might arise are discussed.

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Insertion and excision of the transposable element mariner in Drosophila.

Genetics ◽

10.1093/genetics/125.1.103 ◽

1990 ◽

Vol 125 (1) ◽

pp. 103-114 ◽

Cited By ~ 3

Author(s):

G Bryan ◽

D Garza ◽

D Hartl

Keyword(s):

Transposable Element ◽

Molecular Structures ◽

Sequence Specificity ◽

Wild Type ◽

Absolute Requirement ◽

Wide Range ◽

Drosophila Mauritiana ◽

White Locus ◽

Polymerase Chain ◽

Derivatives Of

Abstract The transposable element mariner is active in both germline and somatic cells of Drosophila mauritiana. Activity of the element is greatly enhanced in the presence of Mos1, a genetic factor identified as an autonomous copy of mariner. A strain of D. mauritiana containing Mos1 and other copies of mariner was used to initiate a screen for visible mutations. More than 20 mutations were obtained, including alleles of white, yellow and vermilion. Six alleles were characterized at the molecular level, and all were found to contain a mariner element inserted into the affected gene. Four insertions into the white locus were sequenced to determine the exact site of insertion of mariner. There appears to be little sequence specificity requirement for mariner insertion, other than an absolute requirement for the dinucleotide TA, which is duplicated upon insertion. Sequences of phenotypically wild-type germline and somatic revertants obtained from various white alleles, including the previously isolated wpch allele, were obtained using the polymerase chain reaction. Mariner excision is imprecise in both germline and soma, and the most frequent excision events are the same in the two tissues. Mutant derivatives of wpch were also studied, and were found to exhibit a wide range of molecular structures and phenotypes.

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Interaction between Agrobacterium tumefaciens oncoprotein 6b and a tobacco nucleolar protein that is homologous to TNP1 encoded by a transposable element of Antirrhinum majus

Journal of Plant Research ◽

10.1007/s10265-008-0160-1 ◽

2008 ◽

Vol 121 (4) ◽

pp. 425-433 ◽

Cited By ~ 13

Author(s):

Saeko Kitakura ◽

Shinji Terakura ◽

Yasushi Yoshioka ◽

Chiyoko Machida ◽

Yasunori Machida

Keyword(s):

Agrobacterium Tumefaciens ◽

Transposable Element ◽

Nucleolar Protein ◽

Antirrhinum Majus

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TAM (transposable element Antirrhinum majus)

10.1007/springerreference_107067 ◽

2012 ◽

Keyword(s):

Transposable Element ◽

Antirrhinum Majus

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Molecular analysis of a transposon-induced deletion of the nivea locus in Antirrhinum majus.

Genetics ◽

10.1093/genetics/123.2.417 ◽

1989 ◽

Vol 123 (2) ◽

pp. 417-425

Author(s):

C Lister ◽

C Martin

Keyword(s):

Transposable Element ◽

Molecular Analysis ◽

Dna Sequences ◽

Large Scale ◽

Large Deletion ◽

Antirrhinum Majus ◽

Coding Region ◽

Genomic Position

Abstract The transposable element Tam3 of Antirrhinum majus is capable of causing large-scale chromosomal restructuring. It induced a large deletion at the nivea locus, to produce the allele niv-:529. The deletion removed the entire nivea coding region while the element remains intact with the potential to induce further rearrangements. Genetic experiments showed that the endpoint of the deletion (called x) is closely linked to nivea. The DNA sequences of niv-:529, a genomic excision of Tam3 from niv-:529, and the original genomic position of x have been determined. These data suggest that the deletion could have resulted from an abortive transposition or through breakage and religation.

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