Heritable fragile sites on human chromosomes

1979 ◽  
Vol 53 (1) ◽  
pp. 23-27 ◽  
Author(s):  
G. R. Sutherland
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

A Novel Technique for Observing the Internal Ultrastructure of Human Chromosomes with Known Karyotype

2008 ◽  
Vol 14 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Mohammad Ghazizadeh ◽  
Yoshihiro Sasaki ◽  
Tatsuo Oguro ◽  
Shigeru Sato ◽  
Seiko Egawa ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Osmium Tetroxide ◽  
Human Lymphocytes ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Chromosome Research ◽  
Novel Technique ◽  
Transmission Electron ◽  
Comprehensive Information ◽  
Normal Human

Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. In this study we have used a method in which metaphase chromosome spreads were prepared on a flexible thermoplastic membrane (ACLAR) film. To assess chromosome identity, a diamidino-phenylindole staining and karyotying was first done using a conventional cytogenetic system. The chromosome spreads were then fixed with 1% osmium tetroxide, stained with freshly prepared 2% tannic acid, dehydrated, and flat-embedded in epoxy resin. The resin sheet was easily detachable and carried whole chromosome spreads. By this method, TEM observation of chromosomes from normal human lymphocytes allowed a thorough examination of the ultrastructure of centromeres, telomeres, fragile sites, and other chromosomal regions. Various ultrastructural patterns including thick electron dense boundaries, less dense internal regions, and extended chromatin loops at the periphery of the chromosomes were discernible. Application of the present method to chromosome research is expected to provide comprehensive information on the internal ultrastructure of different chromosomal regions in relation to function.

Fragile sites on human chromosomes

Pathology ◽  
1981 ◽  
Vol 13 (1) ◽  
pp. 179 ◽  
Author(s):  
Grant R. Sutherland
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

On the nature of folic-acid-sensitive fragile sites in human chromosomes: An hypothesis

1983 ◽  
Vol 16 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Carlos L. Krumdieck ◽  
Patricia N. Howard-Peebles ◽  
John M. Opitz
Keyword(s):  
Folic Acid ◽  
Fragile Sites ◽  
Human Chromosomes

scholarly journals Fragile Sites on Human Chromosomes

1986 ◽  
Vol 23 (3) ◽  
pp. 285-285
Author(s):  
T. Webb
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

scholarly journals HumCFS: a database of fragile sites in human chromosomes

BMC Genomics ◽  
2019 ◽  
Vol 19 (S9) ◽  
Author(s):  
Rajesh Kumar ◽  
Gandharva Nagpal ◽  
Vinod Kumar ◽  
Salman Sadullah Usmani ◽  
Piyush Agrawal ◽  
...  
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

DNA polymerase ? inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes

1984 ◽  
Vol 67 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Thomas W. Glover ◽  
Carol Berger ◽  
Jane Coyle ◽  
Barbara Echo
Keyword(s):  
Dna Polymerase ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Common Fragile Sites

Enigmatic fragile sites on human chromosomes

1988 ◽  
Vol 4 (5) ◽  
pp. 121 ◽  
Author(s):  
Frederick Hecht
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

The clinical significance of fragile sites on human chromosomes

2001 ◽  
Vol 58 (3) ◽  
pp. 157-161 ◽  
Author(s):  
GR Sutherland ◽  
E Baker
Keyword(s):  
Clinical Significance ◽  
Fragile Sites ◽  
Human Chromosomes

Synergism between aphidicolin and adenoviruses in the induction of breaks at fragile sites on human chromosomes

1991 ◽  
Vol 54 (1) ◽  
pp. 39-53 ◽  
Author(s):  
D. Caporossi ◽  
S. Bacchetti ◽  
B. Nicoletti
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes

Heritable fragile sites on human chromosomes

1979 ◽  
Vol 53 (1) ◽  
pp. 29-30 ◽  
Author(s):  
G. R. Sutherland ◽  
P. Leonard
Keyword(s):  
Fragile Sites ◽  
Human Chromosomes
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