Differential inhibition of downstream gene expression by the cauliflower mosaic virus 35S RNA leader

Virus Genes ◽  
1989 ◽  
Vol 3 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Johannes F�tterer ◽  
Karl Gordon ◽  
Pierre Pfeiffer ◽  
H�l�lene Sanfa�on ◽  
Barbara Pisan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Differential Inhibition ◽  
Downstream Gene Expression ◽  
Cauliflower Mosaic Virus 35S

scholarly journals Effect of CWG methylation on expression of plant genes

1999 ◽  
Vol 341 (3) ◽  
pp. 473-476 ◽  
Author(s):  
Sriharsa PRADHAN ◽  
Nigel A. R. URWIN ◽  
Gareth I. JENKINS ◽  
Roger L. P. ADAMS
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Dna Methyltransferases ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Promoter Sequences ◽  
Plant Genes ◽  
Transcription Expression ◽  
Cauliflower Mosaic Virus 35S

The presence of two DNA methyltransferases in Pisum raises the possibility that they serve different functions. In vitro methylation of CWG sequences in the strong cauliflower mosaic virus 35S promoter had no effect on reporter gene expression. In contrast, in vitro methylation of CWG sequences in the relatively weak, CG-deficientPhaseolus vulgaris rbcS2 promoter inhibited transcription. Expression of both constructs was strongly inhibited by extensive CG methylation. A search of published plant promoter sequences revealed that the CG content of promoters is very variable, with some promoters having typical CG islands. In contrast, the distribution of CWG sequences is more even with little evidence for CWG islands.

scholarly journals Altered Patterns of Gene Expression in Arabidopsis Elicited by Cauliflower Mosaic Virus (CaMV) Infection and by a CaMV Gene VI Transgene

1999 ◽  
Vol 12 (5) ◽  
pp. 377-384 ◽  
Author(s):  
Chiara Geri ◽  
Edi Cecchini ◽  
Maria E. Giannakou ◽  
Simon N. Covey ◽  
Joel J. Milner
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Mosaic Virus ◽  
Rna Binding ◽  
Important Determinant ◽  
Blot Hybridization ◽  
Cauliflower Mosaic Virus ◽  
Slot Blot Hybridization ◽  
Pcr Products ◽  
Polymerase Chain

Cauliflower mosaic virus (CaMV) gene VI protein (P6) is an important determinant of symptom expression. Differential display polymerase chain reaction (PCR) was used to identify changes in gene expression in Arabidopsis elicited by a P6 transgene that causes a symptomatic phenotype. We used slot blot hybridization to measure the abundance of mRNAs complementary to 66 candidate PCR products in transgenic, CaMV-infected, and uninfected Arabidopsis plants. CaMV-infected and P6 transgenic plants showed broadly similar changes in abundance of mRNA species. In P6 transgenic plants we detected 18 PCR products that showed unambiguous changes in abundance plus another 15 that showed more limited changes (approximately twofold). CaMV-infected plants showed 17 unambiguous and 13 limited changes. Down-regulated species include those encoding a novel, phenol-like sulfotransferase, and a glycine-rich, RNA-binding protein. Up-regulated species included ones encoding an myb protein, glycine-rich and stress-inducible proteins, and a member of a previously unreported gene family. CaMV infection causes alterations in expression of many Arabidopsis genes. Transgene-mediated expression of P6 mimics virus infection in its effect on host gene expression, providing a potential mechanism for this process.

Functional analysis of cauliflower mosaic virus 35S promoter: re-evaluation of the role of subdomains B5, B4 and B2 in promoter activity

2007 ◽  
Vol 5 (6) ◽  
pp. 696-708 ◽  
Author(s):  
Simran Bhullar ◽  
Sudipta Datta ◽  
Sonia Advani ◽  
Suma Chakravarthy ◽  
Taru Gautam ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Mosaic Virus ◽  
Promoter Activity ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Cauliflower Mosaic Virus 35S

Cauliflower mosaic virus as a gene expression vector for plants

1990 ◽  
Vol 79 (1) ◽  
pp. 154-157 ◽  
Author(s):  
J. Futterer ◽  
J. M. Bonneville ◽  
T. Hohn
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Expression Vector ◽  
Cauliflower Mosaic Virus

scholarly journals The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region

2004 ◽  
Vol 78 (22) ◽  
pp. 12120-12128 ◽  
Author(s):  
Sandra Pauli ◽  
Helen M. Rothnie ◽  
Gang Chen ◽  
Xiaoyuan He ◽  
Thomas Hohn
Keyword(s):  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Sequence Motifs ◽  
Transcription Start ◽  
Transcriptional Enhancers ◽  
Enhance Gene Expression ◽  
Enhancer Function ◽  
Nucleotide Region ◽  
Cauliflower Mosaic Virus 35S

ABSTRACT A 60-nucleotide region (S1) downstream of the transcription start site of the cauliflower mosaic virus 35S RNA can enhance gene expression. By using transient expression assays with plant protoplasts, this activity was shown to be at least partially due to the effect of transcriptional enhancers within this region. We identify sequence motifs with enhancer function, which are normally masked by the powerful upstream enhancers of the 35S promoter. A repeated CT-rich motif is involved both in enhancer function and in interaction with plant nuclear proteins. The S1 region can also enhance expression from heterologous promoters.

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