Effect of different fixatives on Con A surface receptors of mouse peritoneal macrophages

1990 ◽  
Vol 93 (4) ◽  
pp. 443-446 ◽  
Author(s):  
Th. Peschke ◽  
L. Wollweber ◽  
A. Gabert ◽  
K. Augsten ◽  
R. Stracke
Keyword(s):  
Peritoneal Macrophages ◽  
Con A ◽  
Surface Receptors ◽  
Mouse Peritoneal Macrophages

Structural heterogeneity of endocytic membranes in macrophages as revealed by the cholesterol probe, filipin

1981 ◽  
Vol 51 (1) ◽  
pp. 95-107
Author(s):  
R. Montesano ◽  
P. Vassalli ◽  
L. Orci
Keyword(s):  
Structural Heterogeneity ◽  
Cholesterol Content ◽  
Peritoneal Macrophages ◽  
Con A ◽  
Coated Pits ◽  
Mechanisms Of Formation ◽  
Smooth Membrane ◽  
Endocytic Vesicles ◽  
Cytochemical Technique ◽  
Mouse Peritoneal Macrophages

The polyene antibiotic, filipin, by specifically interacting with cholesterol, produces approximately 25-nm protuberances (filipin-sterol complexes) in freeze-fractured membranes, and the addition of filipin to aldehyde fixatives has been recently introduced as a cytochemical technique for the localization of cholesterol in cell membranes. In a previous study we showed that, in fibroblasts filipin-sterol complexes are absent from endocytic coated pits. To establish whether the absence of filipin-sterol complexes is a phenomenon restricted to coated pits or is correlated with endocytosis in general, we applied the filipin probe to cultured mouse peritoneal macrophages, in which different forms of endocytosis take place. The macrophages were incubated with bovine albumin or concanavalin A (Con A) to induce pinocytosis, and with heat-killed straphylococci or opsonized erythrocytes to induce phagocytosis, then fixed in glutaraldehyde/filipin and freeze-fractured. Filipin-sterol complexes were plentiful on the plasma membrane, on the smooth-membrane invaginations and vesicles induced by albumin, on the large endocytic vacuoles induced by Con A, and on the membrane of phagosomes but, in contrast, they were absent from coated pits and vesicles, as well as from coated segments of invagination or vesicles. These results indicate that the membranes involved in different types of endocytosis do not react in the same way with filipin and may, therefore, have a different cholesterol content. This could reflect different mechanisms of formation for the various types of endocytic vesicles.

scholarly journals EFFECTS OF CONCANAVALIN A ON MOUSE PERITONEAL MACROPHAGES

1974 ◽  
Vol 140 (5) ◽  
pp. 1364-1386 ◽  
Author(s):  
Paul J. Edelson ◽  
Zanvil A. Cohn
Keyword(s):  
Concanavalin A ◽  
Peritoneal Macrophages ◽  
Surface Marker ◽  
Marker Enzyme ◽  
Apparent Effect ◽  
Con A ◽  
Cytoplasmic Vesicles ◽  
Secondary Lysosomes ◽  
Mouse Peritoneal Macrophages ◽  
Pinocytic Vesicles

Concanavalin A (Con A) binds to saccharide residues on the mouse peritoneal macrophage plasma membrane and stimulates extensive pinocytic interiorization of the membrane. The overall pinocytic rate is increased 3.5–4.5 times by the addition of Con A, and the surface marker enzyme adenosine triphosphatase can be identified histochemically in association with the cytoplasmic vesicles generated after exposure of the cells to Con A. Once formed, these pinocytic vesicles may persist for several days and fail to show morphologic evidence of fusion with primary or preformed secondary lysosomes. There is no apparent effect on the capacity of the macrophage to ingest either latex particles or IgG-coated SRBC administered either simultaneously with or subsequent to the Con A.

scholarly journals THE CYTOTOXIC EFFECT OF MOUSE MACROPHAGES ON SYNGENEIC AND ALLOGENEIC ERYTHROCYTES

1973 ◽  
Vol 137 (3) ◽  
pp. 807-820 ◽  
Author(s):  
H. Melsom ◽  
R. Seljelid
Keyword(s):  
Cytotoxic Effect ◽  
Peritoneal Macrophages ◽  
Target Cells ◽  
Activated Macrophages ◽  
Con A ◽  
Osmotic Effect ◽  
Prolonged Cultivation ◽  
Cytotoxic Reaction ◽  
Mouse Macrophages ◽  
Mouse Peritoneal Macrophages

A cytotoxic effect of mouse peritoneal macrophages against syngeneic and allogeneic erythrocytes was demonstrated by isotope release and release of hemoglobin. The cytotoxic effect was dependent on the contact between viable, activated macrophages and target cells. Activation was accomplished by prolonged cultivation of macrophages and by the presence of Zn++ and Con-A. Immunization did not prove necessary. Morphological observations as well as experiments with various salt concentrations indicate that the cytotoxic reaction may involve some kind of osmotic effect upon the target cells.

scholarly journals Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages

Biomedicines ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 274
Author(s):  
Luis Gil-de-Gómez ◽  
Patricia Monge ◽  
Juan P. Rodríguez ◽  
Alma M. Astudillo ◽  
María A. Balboa ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Peritoneal Macrophages ◽  
Lipid Mediator ◽  
Membrane Phospholipids ◽  
Lipid Profiling ◽  
Surface Receptors ◽  
Cytosolic Phospholipase ◽  
Mouse Peritoneal Macrophages ◽  
Phospholipid Species ◽  
Potential Use

Macrophages contain large amounts of arachidonic acid (AA), which distributes differentially across membrane phospholipids. This is largely due to the action of coenzyme A-independent transacylase (CoA-IT), which transfers the AA primarily from diacyl choline-containing phospholipids to ethanolamine-containing phospholipids. In this work we have comparatively analyzed glycerophospholipid changes leading to AA mobilization in mouse peritoneal macrophages responding to either zymosan or serum-opsonized zymosan (OpZ). These two phagocytic stimuli promote the cytosolic phospholipase A2-dependent mobilization of AA by activating distinct surface receptors. Application of mass spectrometry-based lipid profiling to identify changes in AA-containing phospholipids during macrophage exposure to both stimuli revealed significant decreases in the levels of all major choline phospholipid molecular species and a major phosphatidylinositol species. Importantly, while no changes in ethanolamine phospholipid species were detected on stimulation with zymosan, significant decreases in these species were observed when OpZ was used. Analyses of CoA-IT-mediated AA remodeling revealed that the process occurred faster in the zymosan-stimulated cells compared with OpZ-stimulated cells. Pharmacological inhibition of CoA-IT strongly blunted AA release in response to zymosan but had only a moderate effect on the OpZ-mediated response. These results suggest a hitherto undescribed receptor-dependent role for CoA-independent AA remodeling reactions in modulating the eicosanoid biosynthetic response of macrophages. Our data help define novel targets within the AA remodeling pathway with potential use to control lipid mediator formation

scholarly journals EFFECTS OF CONCANAVALIN A ON MOUSE PERITONEAL MACROPHAGES

1974 ◽  
Vol 140 (5) ◽  
pp. 1387-1403 ◽  
Author(s):  
Paul J. Edelson ◽  
Zanvil A. Cohn
Keyword(s):  
Preceding Paper ◽  
Peritoneal Macrophages ◽  
Anomalous Behavior ◽  
Parasitic Infections ◽  
Half Time ◽  
Lysosomal Hydrolases ◽  
Con A ◽  
Inner Surface ◽  
Mouse Peritoneal Macrophages

The half-time for the degradation of horseradish peroxidase (HRP) is increased from 14 h to 37 h in Con A-treated cells, while the half-time for the degradation of [125I]BSA is increased from 5.4 h to 14.8 h. This supports prior microscopic observations which suggested that Con A pinosomes showed a marked impairment in their ability to form phagolysosomes. Artifacts due to anomalous behavior of HRP-Con A complexes, or to inhibition of lysosomal hydrolases by Con A, could be excluded. These indications of impaired phagolysosome formation, as well as those described in the preceding paper, could be reversed by postincubation of the cells in mannose, but not in galactose. This reversal is accompanied by a dissociation of Con A-FITC from the inner surface of the pinosome membrane, into the vesicle contents. These observations may be relevant to the ability of Con A to affect several membrane characteristics, and are also of interest in relation to the impaired formation of phagolysosomes which has been described in certain in vitro parasitic infections of macrophages or other cells.

scholarly journals Cellular Differentiation in a Murine Myelomonocytic Leukemia

Blood ◽  
1972 ◽  
Vol 39 (6) ◽  
pp. 771-777 ◽  
Author(s):  
M. J. Cline ◽  
D. Metcalf
Keyword(s):  
Cellular Differentiation ◽  
Low Frequency ◽  
Peritoneal Macrophages ◽  
Leukemic Cells ◽  
Macrophage Cell ◽  
Surface Receptors ◽  
Igg Receptors ◽  
Myelomonocytic Leukemia ◽  
Mouse Peritoneal Macrophages

Abstract Mouse peritoneal macrophages and macrophages derived from normal bone marrow progenitors in vitro possess surface receptors whose predominant specificity is directed toward the IgG2a subclass of IgG moleclues. Myelomonocytic leukemic cells also possess such IgG receptors but in an abnormally low frequency and without demonstrable subclass specificity. Within the leukemic population, monoblasts are distinguished from myeloblasts by their greater adhesiveness to glass, higher frequency of cells with surface receptors for IgG, and by erythrophagocytosis. Leukemic monoblasts, myeloblasts, and their morphologically mature progeny demonstrate impaired phagocytic ability. A concept of the development of surface receptors for IgG in the monocyte-macrophage cell line is presented.

Immunostimulatory Effects of Purple Bamboo Salts Composed with Rubus coreanus in Raw264.7 Cells and Mouse Peritoneal Macrophages

2017 ◽  
Vol 46 (3) ◽  
pp. 306-313
Author(s):  
Heejeon Park ◽  
Sokho Kim ◽  
Sohee Jeong ◽  
Heeran Park ◽  
Jin-Hyung Kim ◽  
...  
Keyword(s):  
Peritoneal Macrophages ◽  
Raw264.7 Cells ◽  
Rubus Coreanus ◽  
Mouse Peritoneal Macrophages

Harvest and Culture of Mouse Peritoneal Macrophages

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (22) ◽  
Author(s):  
Mingfang Lu ◽  
Alan Varley
Keyword(s):  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages
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