Immunostimulatory Effects of Purple Bamboo Salts Composed with Rubus coreanus in Raw264.7 Cells and Mouse Peritoneal Macrophages

2017 ◽  
Vol 46 (3) ◽  
pp. 306-313
Author(s):  
Heejeon Park ◽  
Sokho Kim ◽  
Sohee Jeong ◽  
Heeran Park ◽  
Jin-Hyung Kim ◽  
...  
Keyword(s):  
Peritoneal Macrophages ◽  
Raw264.7 Cells ◽  
Rubus Coreanus ◽  
Mouse Peritoneal Macrophages

Distinct arginase isoforms expressed in primary and transformed macrophages: regulation by oxygen tension

1998 ◽  
Vol 274 (3) ◽  
pp. R775-R782 ◽  
Author(s):  
Claudine A. Louis ◽  
Jonathan S. Reichner ◽  
William L. Henry ◽  
Balduino Mastrofrancesco ◽  
Tomomi Gotoh ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Molecular Mechanisms ◽  
Primary Cultures ◽  
Peritoneal Macrophages ◽  
Arginase Activity ◽  
Raw264.7 Cells ◽  
Intact Cells ◽  
Cell Lysates ◽  
Mouse Peritoneal Macrophages ◽  
Lps Treatment

Experiments were performed to identify arginase isoforms expressed in primary and transformed rodent macrophages and to determine the molecular mechanisms for the previously observed increase in arginase activity in macrophages cultured in hypoxia or anoxia. Results demonstrate the following: 1) mRNA and protein for hepatic-type AI arginase are expressed in primary cultures of rat and mouse peritoneal macrophages and are enhanced seven- and ninefold, respectively, by lipopolysaccharide (LPS). 2) mRNA for extrahepatic-type AII arginase is constitutively expressed in mouse, but not rat, peritoneal macrophages and is detected in RAW264.7 cells after LPS treatment; neither J774A.1 nor P388D1 cells contain arginase mRNA. 3) AI arginase mRNA, arginase activity in cell lysates, andl-arginine flux through arginase in intact cells are all increased in rat wound-derived and mouse peritoneal macrophages by hypoxic or anoxic culture; AII arginase mRNA is, in contrast, suppressed >50% by O2deprivation. 4) Expression of thel-arginine transporter mCAT-2 is increased greater than twofold by reduced O2 culture. These results demonstrate substantial variability in arginase isoform expression among primary and transformed rodent macrophages. They also identify AI and AII arginase and the mCAT-2 l-arginine transporter as O2-regulated genes.

scholarly journals DOP67 CKD-506, a selective histone deacetylase (HDAC) 6 inhibitor, ameliorates colitis through regulation of NF-kB and AP-1 signalling pathway

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S105-S105
Author(s):  
J Shin ◽  
N Ha ◽  
D Bae ◽  
Y J Lee ◽  
Y I Choi ◽  
...  
Keyword(s):  
T Cell ◽  
Inflammatory Cytokines ◽  
Inflammatory Mediators ◽  
Peritoneal Macrophages ◽  
Raw264.7 Cells ◽  
Cell Transfer ◽  
Cytokines And Chemokines ◽  
Hdac6 Inhibitor ◽  
T Cell Transfer ◽  
Mouse Peritoneal Macrophages

Abstract Background HDAC6 is a stress-inducible gene and highly expressed in pathological conditions as well as inflammatory bowel disease. Immuno-modulatory functions of HDAC6 inhibitors are well established and proposed therapeutic effects for autoimmune diseases through regulation of Treg cell function and inflammation. Moreover, HDAC6 inhibitors regulate inflammatory cytokines and chemokines, neutrophil activities, and epithelial regeneration in colitis models. CKD-506, a potent and selective oral HDAC6 inhibitor, is generally safe and well-tolerated in human, and is now investigating the efficacy in patients with rheumatoid arthritis. Herein, for future investigation with IBD, we identified molecular action mechanisms of CKD-506 involved in anti-colitis effects. Methods Mouse peritoneal macrophages or Raw264.7 cells were transfected with HDAC6 overexpression plasmid or empty vector as control. Cells were cultured in the presence or absence of 0.03~3 μM CKD-506, and the expression and production of inflammatory mediators were determined by RT–PCR and ELISA respectively. For reporter assays, Raw264.7 cells were transfected with pNF-kB-luc or pAP-1-luc plasmid and luciferase activity in cell lysates was determined by a luminometer. Signalling molecules in HDAC6 overexpressed cells were checked by immunoblot analysis. For the efficacy test of CKD-506, we used DSS-, TNBS-, Piroxicam (IL-10−/−)-, and adaptive T-cell transfer (RAG1−/−)-mediated colitis animal models. Colitis animals were treated with 1 to 100 mg/kg of CKD-506 and analysed disease activities and inflammatory mediators. Results In vivo, CKD-506 strongly inhibited disease activities in DSS-, TNBS-, Piroxicam-, and adaptive T-cell transfer-mediated colitis. In the chemical-induced colitis model, the expression of cell adhesion molecules and chemokines such as IP-10 and also infiltration of immune cells to colon tissues were reduced in CKD-506 treated mice. In vitro, HDAC6 overexpression strongly induced ROS and NADPH oxidase activity in Raw264.7 cells and CKD-506 significantly and dose-dependently inhibited HDAC6-mediated ROS and NADPH activity. Moreover, CKD-506 inhibited the production of pro-inflammatory cytokines and chemokines which are up-regulated in HDAC6 overexpressed mouse peritoneal macrophages or Raw264.7 cells. In promoter assay, HDAC6 overexpression highly induced NF-kB and AP-1 activity and CKD-506 strongly and dose-dependently inhibited both signalling pathways. Conclusion These data provide insight that CKD-506, a selective HDAC6 inhibitor, has anti-inflammatory and anti-colitis effects through regulation of NF-kB and AP-1 signalling pathway. Therefore, CKD-506 may provide beneficial effects in patients with Crohn’s disease and ulcerative colitis.

scholarly journals Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Long Chen ◽  
Xichun Peng ◽  
Jiaying Lv ◽  
Siyin Liao ◽  
Shiyi Ou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Functional Food ◽  
Peritoneal Macrophages ◽  
Water Soluble ◽  
Raw264.7 Cells ◽  
Molar Ratio ◽  
Immunostimulating Activity ◽  
Ft Ir ◽  
Mouse Peritoneal Macrophages

Polysaccharide is one of the important active ingredients of Cantharellus cibarius. The aims of this work were to analyze preliminary characterization and to investigate immunostimulating activity of a novel water-soluble neutral polysaccharide named JP1, which was purified from the fruiting body of Cantharellus cibarius using DEAE-FF chromatography and Sephadex G-100 chromatography. The characteristics of JP1 were determined by HPGPC, FT-IR spectra, gas chromatography, and Congo Red Method. Immunostimulating activity of JP1 was investigated in RAW264.7 cells. Results indicated that JP1 consisted of L-Arabinose, D-Mannose, D-Glucose, and D-Galactose in a molar ratio of 1 : 1.06 : 1.95 : 1.17 with a molecular weight of 336 kDa. JP1 is nontoxic to RAW264.7 cells at this concentration range (62.5–1000 μg/mL). Furthermore, JP1 can promote mouse peritoneal macrophages to secrete NO and enhance the secretion of macrophages’ cytokines IL-6 in RAW264.7 cells. These results suggested that JP1 could have potential immunostimulating activity applications as medicine or functional food.

Harvest and Culture of Mouse Peritoneal Macrophages

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (22) ◽  
Author(s):  
Mingfang Lu ◽  
Alan Varley
Keyword(s):  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages

scholarly journals Neutral amino acid transport in mouse peritoneal macrophages.

1987 ◽  
Vol 262 (27) ◽  
pp. 13015-13019
Author(s):  
H Sato ◽  
H Watanabe ◽  
T Ishii ◽  
S Bannai
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Peritoneal Macrophages ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
Mouse Peritoneal Macrophages
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