Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion

1984 ◽  
Vol 197 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Prem P. Gupta ◽  
Otto Schieder ◽  
Manju Gupta
Keyword(s):  
Gene Transfer ◽  
Protoplast Fusion ◽  
Nuclear Gene ◽  
Asymmetric Protoplast Fusion

Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion

1989 ◽  
Vol 78 (2) ◽  
pp. 194-200 ◽  
Author(s):  
M. D. Sacristán ◽  
M. Gerdemann-Knörck ◽  
O. Schieder
Keyword(s):  
Protoplast Fusion ◽  
Brassica Nigra ◽  
Hygromycin Resistance ◽  
Asymmetric Protoplast Fusion

Current Questions of Gene Transfer via Protoplast Fusion in Microorganisms

1983 ◽  
pp. 137-142
Author(s):  
L. Ferenczy
Keyword(s):  
Gene Transfer ◽  
Protoplast Fusion

Introgression of bacterial wilt resistance from Solanum melongena to S . t uberosum through asymmetric protoplast fusion

2016 ◽  
Vol 125 (3) ◽  
pp. 433-443 ◽  
Author(s):  
Ting Liu ◽  
Yan Yu ◽  
Xingkui Cai ◽  
Wei Tu ◽  
Conghua Xie ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Bacterial Wilt ◽  
Solanum Melongena ◽  
Wilt Resistance ◽  
Bacterial Wilt Resistance ◽  
Asymmetric Protoplast Fusion

Gene transfer by protoplast fusion: Expression cloning of rare gene products in mammalian cells

1993 ◽  
Vol 15 (2) ◽  
pp. 69-71
Author(s):  
Lynn Helena Caporale ◽  
Patricia DeHaven
Keyword(s):  
Gene Transfer ◽  
Protoplast Fusion ◽  
Mammalian Cells ◽  
Fusion Expression ◽  
Expression Cloning ◽  
Gene Products ◽  
Rare Gene

scholarly journals Chloroplast acquisition without the gene transfer in kleptoplastic sea slugs, Plakobranchus ocellatus

2020 ◽  
Author(s):  
Taro Maeda ◽  
Shunichi Takahashi ◽  
Takao Yoshida ◽  
Shigeru Shimamura ◽  
Yoshihiro Takaki ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Horizontal Transfer ◽  
Nuclear Gene ◽  
Intestinal Cells ◽  
Algal Food ◽  
Gene Expansion ◽  
Photosynthetic Genes ◽  
Sea Slugs ◽  
Complex Adaptive ◽  
Independent Transformation

AbstractSome sea slugs sequester chloroplasts from algal food in their intestinal cells and photosynthesize for months. This phenomenon, kleptoplasty, poses a question of how the chloroplast retains its activity without the algal nucleus, and there have been debates on the horizontal transfer of algal genes to the animal nucleus. To settle the arguments, we report the genome of a kleptoplastic sea slug Plakobranchus ocellatus and found no evidence that photosynthetic genes are encoded on the nucleus. Nevertheless, we confirmed that photosynthesis prolongs the life of mollusk under starvation. The data present a paradigm that a complex adaptive trait, as typified by photosynthesis, can be transferred between eukaryotic kingdoms by a unique organelle transmission without nuclear gene transfer. Our phylogenomic and transcriptomic analysis showed that genes for proteolysis and immunity underwent gene expansion and are upregulated in the chloroplast-enriched tissue, suggesting that these molluscan genes are involved in this DNA-independent transformation.

Asymmetric protoplast fusion aimed at intraspecific transfer of cytoplasmic male sterility (CMS) in Lolium perenne L.

1992 ◽  
Vol 84-84 (5-6) ◽  
pp. 763-770 ◽  
Author(s):  
J. Creemers-Molenaar ◽  
R. D. Hall ◽  
F. A. Krens
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Protoplast Fusion ◽  
Lolium Perenne ◽  
Lolium Perenne L ◽  
Asymmetric Protoplast Fusion

GENE TRANSFER BY PROTOPLAST FUSION

1983 ◽  
pp. 121-129
Author(s):  
Edward C. Cocking
Keyword(s):  
Gene Transfer ◽  
Protoplast Fusion
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