We studied the biochemical composition, nutritive value of the green mass and prepared hay from Ro-manian cultivars of perennial ryegrass, Lolium perenne L. „Mara” and „Măgura” grown in monoculture on the experimental plot of the National Botanical Garden (Institute) “Alexandru Ciubotaru”, Chisinau, R. Mol-dova. Results revealed that dry matter of harvested green mass contained 98-116 g/kg CP, 299-326 g/kg CF, 71-77 g/kg ash, 329-353 g/kg ADF, 546-593 g/kg NDF, 38-40 g/kg ADL, 291-313 g/kg Cel, 217-240 g/kg HC, with nutritive and energy value 60.9-65.3 % DMD, 60.1-64.8 % ODM, RFV=96-108, 12.12-12.45 MJ/kg DE, 9.95- 10.23 MJ/kg ME and 5.97-6.25 MJ/kg NEl. The biochemical composition, nutritive and energy va-lue of prepared hay: 94-110 g/kg CP, 351-385 g/kg CF, 79-82 g/kg ash, 390-423 g/kg ADF, 631-689 g/kg NDF, 46-50 g/kg ADL, 344-373g/kg Cel, 241-266 g/kg HC, RFV=76-86, 11.13-11.61 MJ/kg DE, 9.14- 9.53 MJ/kg ME and 5.17-5.55 MJ/kg NEl. The biochemical methane potential of green mass substrates reached 338-344 L/kg organic matter. The studied cultivars of Lolium perennec could be used in the Republic of Mol-dova for the restoration of degraded permanent grasslands, as a component of the mix of grasses and legumes for the creation of temporary grasslands, the harvested biomass can be used as feed for farm animals or as substrate in biogas generator for renewable energy production.