Banding pattern analysis of human chromosomes by use of a urea treatment technique

Chromosoma ◽  
1972 ◽  
Vol 37 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Yukimasa Shiraishi ◽  
Tosihide H. Yosida
Keyword(s):  
Banding Pattern ◽  
Pattern Analysis ◽  
Treatment Technique ◽  
Human Chromosomes ◽  
Urea Treatment

Structural basis of banding pattern of human chromosomes

1973 ◽  
Vol 12 (2) ◽  
pp. 81-86 ◽  
Author(s):  
J. Cervenka ◽  
Hattie L. Thorn ◽  
R.J. Gorlin
Keyword(s):  
Banding Pattern ◽  
Structural Basis ◽  
Human Chromosomes

Q-Banding Pattern Analysis of KB Cells

1974 ◽  
Vol 13 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
C.-C. Lin
Keyword(s):  
Banding Pattern ◽  
Pattern Analysis ◽  
Kb Cells

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes ◽  
Nick Translation ◽  
In Situ Nick Translation ◽  
Eco Ri

Telomerase is processive

1991 ◽  
Vol 11 (9) ◽  
pp. 4572-4580
Author(s):  
C W Greider
Keyword(s):  
Banding Pattern ◽  
Tandem Repeats ◽  
Pattern Analysis ◽  
Reaction Products

Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-337, 1989; G.-L. Lu, J. D. Bradley, L. D. Attardi, and E. H. Blackburn, Nature (London) 344:126-132, 1990]. In a typical reaction, products greater than 2,000 nucleotides were synthesized in 60 min. Dilution and primer challenge experiments showed that these long products were synthesized processively. The apparent processivity was not due to a higher affinity of the enzyme for long d(TTGGGG) products over the shorter competitors. The degree of processivity was quantitated; telomerase synthesized approximately 520 nucleotides before half of the enzyme had dissociated. After dissociating, telomerase reinitiated d(TTGGGG)n synthesis on new primer oligonucleotides. The products from a telomerase reaction have a characteristic 6-nucleotide banding pattern (C. W. Greider and E. H. Blackburn, Cell 51:887-898, 1987). A strong pause in the reaction occurs after the addition of the first G in the sequence d(TTGGGG). Both the processivity and the banding pattern analysis imply that in the elongation mechanism there must be a translocation step after the 9 nucleotides of internal template RNA have been copied to the extreme 5' end.

Fluorescent banding pattern analysis of eight taxa of Phaseolus and Vigna in relation to their phylogenetic relationships

1993 ◽  
Vol 87 (1-2) ◽  
pp. 38-43 ◽  
Author(s):  
J. Y. Zheng ◽  
M. Nakata ◽  
K. Irifune ◽  
R. Tanaka ◽  
H. Morikawa
Keyword(s):  
Banding Pattern ◽  
Phylogenetic Relationships ◽  
Pattern Analysis ◽  
Fluorescent Banding

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

Differential Acridine Orange Staining of Human Chromosomes

1972 ◽  
Vol 21 (4) ◽  
pp. 319-326
Author(s):  
G.L. Castoldi ◽  
G.D. Grusovin ◽  
G.L. Scapoli ◽  
R. Spanedda
Keyword(s):  
Acridine Orange ◽  
Banding Pattern ◽  
Human Chromosomes ◽  
Salt Solutions ◽  
Single Stranded Dna ◽  
Acridine Orange Staining ◽  
Double Stranded Dna ◽  
Previously Treated

SummaryThe acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

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