Nucleolar organizer activity in wheat, rye and derivatives analyzed by a silver-staining procedure

Chromosoma ◽  
1984 ◽  
Vol 89 (5) ◽  
pp. 370-376 ◽  
Author(s):  
M. C. Cerme�o ◽  
J. Orellana ◽  
J. L. Santos ◽  
J. R. Lacadena
1985 ◽  
Vol 27 (4) ◽  
pp. 479-483 ◽  
Author(s):  
M. C. Cermeño ◽  
J. R. Lacadena

The nucleolar organizer activity in several Aegilops × rye hybrids (A. triuncialis × Secale cereale, A. variabilis × S. cereale, A. biunicialis × S. cereale, A. biuncialis × S. vavilovii, A. juvenalis × S. cereale) is analyzed by using a highly reproducible silver-staining procedure. The 1U and 5U chromosomes show a strong nucleolar activity, suppressing the NOR activity of chromosome 1R from rye in all the hybrid combinations (UCR, USvR, UMbR, UMbRv, and UMjDR). The nucleolus organizer chromosomes from the genomes C, Sv, Mb, and Mj show small activities. Our results confirm previous data of the nucleolar organizer activity predominant status of the 1U and 5U chromosomes from the U genome (A. umbellulata) and the weakest condition of the 1R chromosome from rye. A diagram showing the relationships between the nucleolar organizer activities of chromosomes from different genomes of Triticeae is presented.Key words: nucleolar competition, amphiplasty, Ag-NORs, Triticeae, Aegilops, Secale.


1986 ◽  
Vol 28 (2) ◽  
pp. 227-234 ◽  
Author(s):  
N. Cuñado ◽  
M. C. Cermeño ◽  
J. Orellana

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.


2008 ◽  
Vol 19 (3) ◽  
pp. 145-148 ◽  
Author(s):  
D. M. Cheng ◽  
T. E. Denton ◽  
S. L. Liem ◽  
C. L. Elliot

1985 ◽  
Vol 99 (4) ◽  
pp. 511-513 ◽  
Author(s):  
N. N. Mamaev ◽  
N. V. Bebiya ◽  
S. E. Mamaeva ◽  
I. A. Chalisov ◽  
I. V. Bykhovets

Caryologia ◽  
2006 ◽  
Vol 59 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Ferrari Maria Rosa ◽  
Maia Fradkin ◽  
Eduardo Josè Greizerstein ◽  
Carlos Alberto Naranjo ◽  
Lidia Poggio

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