Food preference and food type innovation of surface- vs. cave-dwelling waterlouse (Asellus aquaticus) after 60 000 years of isolation

Behavioural innovativeness is important for colonising new habitats; however, it is also costly. Along the colonisation event of a simple, stable and isolated habitat offering only new food sources, one could hypothesize that the colonising individuals are more innovative than the average in their source population, showing preference to the new resource, while after colonisation, the adapted population will lose its innovativeness and become specialised to the new resource. To test this hypothesis, we compared food preference and food type innovation of a cave-dwelling waterlouse (Asellus aquaticus) population (genetically isolated for at least 60 000 years) to three surface-dwelling populations, also sampling individuals that have recently entered the cave (‘colonists’). In the cave, the only food sources are endogenous bacterial mats, while surface populations feed on various living and dead plant material together with their fungal and bacterial overgrow. We assayed all populations with the familiar and unfamiliar food types from the natural habitats and two novel food types not occurring in the natural habitats of the species. We found that all populations preferred surface to cave food and consumed the unnatural novel food types. Surface populations avoided cave food and colonists spent the most time with feeding on surface food. We conclude that the cave population maintained its preference for surface food and did not lose its food type innovativeness. We suggest that adapting to the special cave food was a major challenge in colonising the cave. Significance statement Behavioural innovativeness is a key trait for adapting to environmental changes or to colonise new habitats. However, it has developmental and maintenance costs due to the high energy need of the necessary sensory and neural organs. Therefore, we asked whether behavioural innovativeness decreases after colonising an isolated, stable and highly specialised habitat. By comparing food type innovativeness of surface-dwelling populations of waterlouse (Asellus aquaticus) to a population that has colonised a cave at least 60 000 years ago, we found that the high innovativeness towards unnatural food was retained in the cave population. Further, all populations preferred surface food (decaying leaves), with surface populations almost completely avoiding cave food (endogenous bacteria mats). We suggest that (i) food type innovativeness is evolutionary rigid in our system and (ii) the cave food was rather an obstacle against than a trigger of cave colonisation.

Building on 150 Years of Knowledge: The Freshwater Isopod Asellus aquaticus as an Integrative Eco-Evolutionary Model System

Interactions between organisms and their environments are central to how biological diversity arises and how natural populations and ecosystems respond to environmental change. These interactions involve processes by which phenotypes are affected by or respond to external conditions (e.g., via phenotypic plasticity or natural selection) as well as processes by which organisms reciprocally interact with the environment (e.g., via eco-evolutionary feedbacks). Organism-environment interactions can be highly dynamic and operate on different hierarchical levels, from genes and phenotypes to populations, communities, and ecosystems. Therefore, the study of organism-environment interactions requires integrative approaches and model systems that are suitable for studies across different hierarchical levels. Here, we introduce the freshwater isopod Asellus aquaticus, a keystone species and an emerging invertebrate model system, as a prime candidate to address fundamental questions in ecology and evolution, and the interfaces therein. We review relevant fields of research that have used A. aquaticus and draft a set of specific scientific questions that can be answered using this species. Specifically, we propose that studies on A. aquaticus can help understanding (i) the influence of host-microbiome interactions on organismal and ecosystem function, (ii) the relevance of biotic interactions in ecosystem processes, and (iii) how ecological conditions and evolutionary forces facilitate phenotypic diversification.

Water Lice and Other Macroinvertebrates in Drinking Water Pipes: Diversity, Abundance and Health Risk

Activities to ensure and maintain water quality in drinking water networks, including flushing, are presented after standardized hydrant sampling combined with a stainless-steel low pressure–high flow rate (NDHF) filter and a 100 µm mesh size was used to separate pipe inhabitants. A databank of more than 1000 hydrant samples in European lowland areas was developed and used to analyze the diversity and abundance of macroinvertebrates in drinking water networks. Load classes for water louse (Asellus aquaticus) and oligochaetes are given with three evaluation classes: normal colonization, increased colonization, and mass development. The response of Asellus aquaticus in drinking water networks to environmental conditions are presented as are their growth and reproduction, promotion of a third generation by climate change effects, food limitations, and the composition and stability of their feces. Finally, the health risks posed by dead water lice and water lice feces with bacterial regrowth and the promotion of microbe development on house filters are analyzed.

Population divergence in aggregation and sheltering behaviour in surface and cave-adapted Asellus aquaticus (Crustacea: Isopoda)

Aggregation (gathering together) and sheltering (hiding in cover) are basic behaviours that might reduce the risk of predation. However, both behaviours have costs, like increased competition over resources and high prevalence of contact-spread parasites (aggregation) or lost opportunities for foraging and mating (sheltering). Therefore, adaptive variation in these behaviours is expected between populations with varying levels of predation risk. We compared aggregation and sheltering in surface- (various predators) and cave-adapted (no predator) populations of the Isopod Asellus aquaticus in a common garden experiment. Since the cave environment is constantly dark, we also tested for population variation in light-induced behavioural plasticity by applying light treatments. Variation in sheltering was explained by habitat type: cave individuals sheltered less than surface individuals. We found high between-population variation in aggregation with or without shelters and their light-induced plasticity, which were unexplained by habitat type. Cave individuals habituated (decreased sheltering), while surface individuals showed sensitization (increased sheltering). We suggest that population variation in sheltering is driven by predation, while variation in aggregation must be driven by other, unaccounted environmental factors, similarly to light-induced behavioural plasticity. Based on habituation/sensitization patterns, we suggest that predation-adapted populations are more sensitive to disturbance related to standard laboratory procedures.