Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae

1984 ◽  
Vol 193 (3) ◽  
pp. 507-512 ◽  
Author(s):  
Richard B. Bailey ◽  
Anne Woodword
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Repression ◽  
Resistant Mutant ◽  
Isolation And Characterization

Isolation and characterization of an uncoupler-resistant mutant of Saccharomyces cerevisiae

1984 ◽  
Vol 8 (7) ◽  
pp. 507-516 ◽  
Author(s):  
Charles-Henri Dupont ◽  
Roland Caubet ◽  
Jean-Pierre Mazat ◽  
Bernard Guerin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Resistant Mutant ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of mutants which show an oversecretion phenotype in Saccharomyces cerevisiae.

Genetics ◽  
1988 ◽  
Vol 119 (3) ◽  
pp. 499-506
Author(s):  
A Sakai ◽  
Y Shimizu ◽  
F Hishinuma
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Repression ◽  
Temperature Sensitive ◽  
Cellular Functions ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Complementation Groups ◽  
Homozygous Diploid ◽  
Diploid Cells

Abstract We have isolated mutants responsible for an oversecretion phenotype in Saccharomyces cerevisiae, using a promoter of SUC2 and the gene coding for alpha-amylase from mouse as a marker of secretion. These mutations defined two complementation groups, designated as ose1 (over secretion) and rgr1 (resistant to glucose repression). The ose1 mutant produced an oversecretion of amylase by 12- to 15-fold under derepressing conditions; however, the amylase mRNA was present at nearly the same amount as it was in the parent cells. No expression of the amylase gene was detected under repressing conditions. The rgr1 mutant oversecreted amylase by 11- to 13-fold under repressing conditions by 15- to 18-fold under derepressing conditions. The rgr1 mutant showed pleiotropic effects on the following cellular functions: (1) resistance to glucose repression, (2) temperature-sensitive lethality, (3) sporulation deficieny in homozygous diploid cells, and (4) abnormal cell morphology. The rgr1 mutation was not allelic with ssn6 and cyc9, and failed to suppress snf1.

Isolation and characterization of the positive regulatory gene ADR1 from Saccharomyces cerevisiae

1983 ◽  
Vol 3 (3) ◽  
pp. 360-370
Author(s):  
C L Denis ◽  
E T Young
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Restriction Site ◽  
Glucose Repression ◽  
Regulatory Gene ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Site Mapping ◽  
Nonfermentable Carbon Source ◽  
Restriction Site Mapping

The DNA segments containing the ADR1 gene and a mutant allele, ADR1-5c, have been isolated by complementation of function in Saccharomyces cerevisiae. The ADR1 gene is required for synthesis of the glucose-repressible alcohol dehydrogenase (ADHII) when S. cerevisiae cells are grown on a nonfermentable carbon source, whereas the ADR1-5c allele allows ADHII synthesis even during glucose repression. A plasmid pool consisting of yeast DNA fragments isolated from a strain carrying the ADR1-5c allele was used to transform a strain containing the adr1-1 allele, which prevents ADHII depression. Transformants were isolated which expressed ADHII during glucose repression. A plasmid isolated from one of these transformants was shown to carry the ADR1-5c allele by its ability to integrate at the chromosomal adr1-1 locus. The wild-type ADR1 gene was isolated by colony hybridization, using the cloned ADR1-5c gene as a probe. The ADR1-5c and ADR1 DNA segments were indistinguishable by restriction site mapping. A partial ADR1 phenotype could be conferred by a 1.9-kilobase region, but DNA outside of this region appeared to be necessary for normal activation of ADHII by the ADR1 gene.

scholarly journals Isolation and characterization of the positive regulatory gene ADR1 from Saccharomyces cerevisiae.

1983 ◽  
Vol 3 (3) ◽  
pp. 360-370 ◽  
Author(s):  
C L Denis ◽  
E T Young
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Restriction Site ◽  
Glucose Repression ◽  
Regulatory Gene ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Site Mapping ◽  
Nonfermentable Carbon Source ◽  
Restriction Site Mapping

The DNA segments containing the ADR1 gene and a mutant allele, ADR1-5c, have been isolated by complementation of function in Saccharomyces cerevisiae. The ADR1 gene is required for synthesis of the glucose-repressible alcohol dehydrogenase (ADHII) when S. cerevisiae cells are grown on a nonfermentable carbon source, whereas the ADR1-5c allele allows ADHII synthesis even during glucose repression. A plasmid pool consisting of yeast DNA fragments isolated from a strain carrying the ADR1-5c allele was used to transform a strain containing the adr1-1 allele, which prevents ADHII depression. Transformants were isolated which expressed ADHII during glucose repression. A plasmid isolated from one of these transformants was shown to carry the ADR1-5c allele by its ability to integrate at the chromosomal adr1-1 locus. The wild-type ADR1 gene was isolated by colony hybridization, using the cloned ADR1-5c gene as a probe. The ADR1-5c and ADR1 DNA segments were indistinguishable by restriction site mapping. A partial ADR1 phenotype could be conferred by a 1.9-kilobase region, but DNA outside of this region appeared to be necessary for normal activation of ADHII by the ADR1 gene.

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