scholarly journals Isolation and characterization of two distinct myo-inositol transporter genes of Saccharomyces cerevisiae

1991 ◽  
Vol 266 (17) ◽  
pp. 11184-11191
Author(s):  
J. Nikawa ◽  
Y. Tsukagoshi ◽  
S. Yamashita
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Isolation And Characterization

Mutations at the Saccharomyces cerevisiae SUP4 tRNA Tyr Locus: Isolation, Genetic Fine-Structure Mapping, and Correlation with Physical Structure

1982 ◽  
Vol 2 (12) ◽  
pp. 1501-1513
Author(s):  
Janet Kurjan ◽  
Benjamin D. Hall
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Physical Structure ◽  
Mapping Technique ◽  
Structure Mapping ◽  
Isolation And Characterization ◽  
Qualitative Estimate ◽  
Genetic Fine Structure ◽  
Entire Gene

The SUP4 tRNA Tyr locus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at the SUP4 gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus the sup4 + anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and the sup4 + anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell 22 :415-425, 1980; Kurjan et al., Cell 20 :701-709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and “map expansion,” were seen.

scholarly journals Isolation and characterization of a Type II casein kinase (‘casein kinase-TS’) from Saccharomyces cerevisiae

FEBS Letters ◽  
1982 ◽  
Vol 144 (2) ◽  
pp. 354-358 ◽  
Author(s):  
Maria Pia Rigobello ◽  
Elisabetta Jori ◽  
Giovanna Carignani ◽  
Lorenzo A. Pinna
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Casein Kinase ◽  
Type Ii ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document