The frequency and distribution of sister chromatid exchanges in human chromosomes

1977 ◽  
Vol 38 (3) ◽  
pp. 271-278 ◽  
Author(s):  
William F. Morgan ◽  
Peter E. Crossen
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchanges ◽  
Human Chromosomes ◽  
Chromatid Exchanges

scholarly journals MICROFLUOROMETRIC ANALYSIS OF DEOXYRIBONUCLEIC ACID REPLICATION KINETICS AND SISTER CHROMATID EXCHANGES IN HUMAN CHROMOSOMES

1974 ◽  
Vol 22 (7) ◽  
pp. 478-491 ◽  
Author(s):  
SAMUEL A. LATT
Keyword(s):  
High Resolution ◽  
Dna Synthesis ◽  
Sister Chromatid ◽  
Deoxyribonucleic Acid ◽  
Sister Chromatid Exchanges ◽  
Human Chromosomes ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Human Leukocytes ◽  
Chromatid Exchanges

Fluorescence of the dye 33258 Hoechst, when bound to chromosomes, is partially quenched by the incorporation of 5-bromodeoxyuridine into chromosomal deoxyribonucleic acid (DNA). This effect allows microfluorometric analysis of DNA synthesis. Metaphase chromosomes from cultured human leukocytes which have incorporated 5-bromodeoxyuridine for a portion of the DNA synthesis period exhibit reduced 33258 Hoechst fluorescence in 5-bromodeoxyuridine-containing regions. Regions synthesizing DNA during a particular interval can thus be highlighted by the appropriate protocol of 5-bromodeoxyuridine administration. Chromosomes from cells which have replicated twice in medium containing 5-bromodeoxyuridine exhibit one brightly and one dully fluorescing chromatid, reflecting incorporation of 5-bromodeoxyuridine into one or two chains of chromatid DNA, respectively. Sister chromatid exchanges, evident as sharply demarcated reciprocal alterations in fluorescence along chromosomes, can be located relative to quinacrine banding patterns. This fluorometric approach should be useful in many instances as a convenient, high resolution alternative to autoradiography.

Localization of Sister Chromatid Exchanges in Human Chromosomes

Science ◽  
1974 ◽  
Vol 185 (4145) ◽  
pp. 74-76 ◽  
Author(s):  
S. A. Latt
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchanges ◽  
Human Chromosomes ◽  
Chromatid Exchanges

scholarly journals Enhancement by Caffeine of Chromosome Aberrations and Sister Chromatid Exchanges Induced by Mitomycin C in Human Chromosomes

1978 ◽  
Vol 54 (10) ◽  
pp. 618-622
Author(s):  
Yukimasa SHIRAISHI ◽  
Keizo YAMAMOTO ◽  
Avery A. SANDBERG
Keyword(s):  
Mitomycin C ◽  
Sister Chromatid ◽  
Chromosome Aberrations ◽  
Sister Chromatid Exchanges ◽  
Human Chromosomes ◽  
Chromatid Exchanges

Diagnostic Ultrasound and Sister Chromatid Exchanges

1985 ◽  
Vol 40 (9) ◽  
pp. 589-590
Author(s):  
V. CIARAVINO ◽  
A. BRULFERT ◽  
M. W. MILLER ◽  
D. JACOBSON-KRAM ◽  
W. F. MORGAN
Keyword(s):  
Sister Chromatid ◽  
Diagnostic Ultrasound ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges
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