In vitro malignant transformation of in vivo ENU-induced rat ovarian Sertoli cell tumor (adenoma)

1988 ◽  
Vol 114 (2) ◽  
pp. 142-148
Author(s):  
George Stoica ◽  
Marilyn O'Leary
Keyword(s):  
Malignant Transformation ◽  
Sertoli Cell ◽  
Cell Tumor ◽  
Sertoli Cell Tumor

scholarly journals Intra-abdominal Sertoli Cell Tumor in Dog with Local and Systemic Repercussions

2018 ◽  
Vol 46 ◽  
pp. 7
Author(s):  
Mirlla Baracho Ferreira ◽  
Keylla Suellen Batalha Rocha Feranandes ◽  
Alessandra Moreira Da Silva ◽  
Kayana Cunha Marques ◽  
Brizza Zorayd Luz Lopes Rocha ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Testicular Cancer ◽  
Malignant Transformation ◽  
Sertoli Cell ◽  
Abdominal Mass ◽  
Cell Tumor ◽  
Sertoli Cell Tumor ◽  
Left Testis ◽  
Bone Marrow Hypoplasia ◽  
Adjacent Structures

Background: In dogs, abdominal testes have higher risk of malignant transformation, and the Sertoli cell tumor is the most common type of testicular cancer in the species. This neoplasia can lead to an increase on estrogen production, causing a paraneoplastic feminization syndrome with dermatologic, behavioral and blood repercussions. When located inside theabdomen, the Sertoli cell tumor can compress adjacent organs, worsening the prognosis. Thus, this study aimed to report a case of an intra-abdominal Sertoli cell tumor in a dog associated to multiple organic complications.Case: A 4-year-old male Shih-tzu was admitted at the Veterinary Hospital of Universidade Federal Rural do Semi-Árido (UFERSA), with a history of abdominal cryptorchidism and the development of dermatological signs, such as alopecia and hyperpigmentation, with an evolution of two months. At physical examination it was observed alopecia on lumbosacral regionand on hind limbs, hyperpigmentation, gynecomastia and pendulous prepuce, suggestive of feminization syndrome. A firm mass was palpated on caudal abdomen. The following exams were requested: complete blood cell count, serum biochemistry (alanine aminotransferase, alkaline phosphatase, creatinine and urea), abdominal ultrasonography and fine needle aspiration cytology of the abdominal mass. Hematological and biochemistry profile were within the normal range for the species. Onthe ultrasonography, regular and rounded structures were observed, suggesting ectopic testes (left testis: 2.37 x 2.95 cm; right testis: 1.92 x 1.38 cm). The left testis presented characteristics of malignant transformation. The cytology of the abdominal mass suggested Sertoli cell tumor. The dog was submitted to an exploratory laparotomy. The right testis was atrophied and its orchiectomy was performed. The contralateral testis was completed adhered to great vessels, such as abdominal aorta and caudal vena cava, and also to adjacent viscera. Thus, the exeresis of the tumor was not possible and fragments of the mass were collected and referred to histopathology. The left testicle presented tumoral polyedric cells, alonged at the longitudinal axis, propagating disorderly, and with central rounded nucleus, and a clear and vacuolar cytoplasm. It was alsoobserved desmoplasia, anisocytosis, anisocariasis, nuclear atypia and evident nucleoli. The mitotic index was less than one mitotic figure/40x, with aberrant mitotic figures. The histopathological evaluation was compatible with Sertoli cell tumor, confirming the presumptive diagnosis. Chemotherapy was proposed, but the animal’s owner refused. After four months the dog was readmitted presenting a series of complications due to the tumor growth, including bone marrow hypoplasia, gallbladder compression and hydronephrosis. Because of the poor prognosis and severity of complications a decision to euthanize the animal was made.Discussion: Intra-abdominal Sertoli cell tumors have good prognosis when the diagnosis and surgical treatment are made early. Nevertheless, this neoplasia can be associated to critical and irreversible damages to various organs, such as bone marrow hypoplasia, and compression of abdominal organs when the tumor grows rapidly and invades adjacent structures,making the complete surgical exeresis impossible. Therefore, the orchiectomy of cryptorchidic dogs is recommended as soon as possible to prevent the development of testicular cancer in these animals.Keywords: cryptorchidism, testicular neoplasia, Canis lupus familiaris.

scholarly journals Astrocytes derived from p53-deficient mice provide a multistep in vitro model for development of malignant gliomas.

1995 ◽  
Vol 15 (8) ◽  
pp. 4249-4259 ◽  
Author(s):  
A M Yahanda ◽  
J M Bruner ◽  
L A Donehower ◽  
R S Morrison
Keyword(s):  
Genomic Instability ◽  
Malignant Transformation ◽  
Nude Mice ◽  
Malignant Gliomas ◽  
Early Event ◽  
Wild Type ◽  
Early Passage ◽  
Wild Type P53

Loss or mutation of p53 is thought to be an early event in the malignant transformation of many human astrocytic tumors. To better understand the role of p53 in their growth and transformation, we developed a model employing cultured neonatal astrocytes derived from mice deficient in one (p53 +/-) or both (p53 -/-) p53 alleles, comparing them with wild-type (p53 +/+) cells. Studies of in vitro and in vivo growth and transformation were performed, and flow cytometry and karyotyping were used to correlate changes in growth with genomic instability. Early-passage (EP) p53 -/- astrocytes achieved higher saturation densities and had more rapid growth than EP p53 +/- and +/+ cells. The EP p53 -/- cells were not transformed, as they were unable to grow in serum-free medium or in nude mice. With continued passaging, p53 -/- cells exhibited a multistep progression to a transformed phenotype. Late-passage p53 -/- cells achieved saturation densities 50 times higher than those of p53 +/+ cells and formed large, well-vascularized tumors in nude mice. p53 +/- astrocytes exhibited early loss of the remaining wild-type p53 allele and then evolved in a manner phenotypically similar to p53 -/- astrocytes. In marked contrast, astrocytes retaining both wild-type p53 alleles never exhibited a transformed phenotype and usually senesced after 7 to 10 passages. Dramatic alterations in ploidy and karyotype occurred and were restricted to cells deficient in wild-type p53 following repeated passaging. The results of these studies suggest that loss of wild-type p53 function promotes genomic instability, accelerated growth, and malignant transformation in astrocytes.

scholarly journals Ezrin is an Actin Binding Protein That Regulates Sertoli Cell and Spermatid Adhesion During Spermatogenesis

Endocrinology ◽  
2014 ◽  
Vol 155 (10) ◽  
pp. 3981-3995 ◽  
Author(s):  
N. Ece Gungor-Ordueri ◽  
Elizabeth I. Tang ◽  
Ciler Celik-Ozenci ◽  
C. Yan Cheng
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Adherens Junction ◽  
Actin Binding ◽  
Permeability Barrier ◽  
Tight Junction Permeability ◽  
Residual Bodies ◽  
Cell Interface

Abstract During spermatogenesis, the transport of spermatids and the release of sperms at spermiation and the remodeling of the blood-testis barrier (BTB) in the seminiferous epithelium of rat testes require rapid reorganization of the actin-based cytoskeleton. However, the mechanism(s) and the regulatory molecule(s) remain unexplored. Herein we report findings that unfold the functional significance of ezrin in the organization of the testis-specific adherens junction at the spermatid-Sertoli cell interface called apical ectoplasmic specialization (ES) in the adluminal compartment and the Sertoli cell-cell interface known as basal ES at the BTB. Ezrin is expressed at the basal ES/BTB in all stages, except from late VIII to IX, of the epithelial cycle. Its knockdown by RNA interference (RNAi) in vitro perturbs the Sertoli cell tight junction-permeability barrier via a disruption of the actin microfilaments in Sertoli cells, which in turn impeded basal ES protein (eg, N-cadherin) distribution, perturbing the BTB function. These findings were confirmed by a knockdown study in vivo. However, the expression of ezrin at the apical ES is restricted to stage VIII of the cycle and limited only between step 19 spermatids and Sertoli cells. A knockdown of ezrin in vivo by RNAi was found to impede spermatid transport, causing defects in spermiation in which spermatids were embedded deep inside the epithelium, and associated with a loss of spermatid polarity. Also, ezrin was associated with residual bodies and phagosomes, and its knockdown by RNAi in the testis also impeded the transport of residual bodies/phagosomes from the apical to the basal compartment. In summary, ezrin is involved in regulating actin microfilament organization at the ES in rat testes.

Mast cell tumor histamine release following morphine exposure both in vitro and in vivo

2018 ◽  
Vol 45 (6) ◽  
pp. 885.e4
Author(s):  
Taylor Curley ◽  
Pedro Boscan ◽  
Douglas Thamm ◽  
Sam Johnson
Keyword(s):  
Mast Cell ◽  
Histamine Release ◽  
Cell Tumor ◽  
Mast Cell Tumor

scholarly journals Differentiation of human germ cell tumor cells in vivo and in vitro.

1992 ◽  
Vol 25 (5) ◽  
pp. 563-576 ◽  
Author(s):  
JUN-ICHI HATA ◽  
JUNICHIRO FUJIMOTO ◽  
EIZABURO ISHII ◽  
AKIHIRO UMEZAWA ◽  
YASUO KOKAI ◽  
...  
Keyword(s):  
Germ Cell ◽  
Tumor Cells ◽  
Germ Cell Tumor ◽  
Cell Tumor ◽  
Human Germ Cell Tumor

Inoperable Ovarian Sertoli Cell Tumor in an Elderly Patient, Case Report and Review of Literature

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Bedoudou H ◽  
Aboueljaoud B ◽  
Mai A ◽  
Banani A ◽  
Alaoui Lamrani Y ◽  
...  
Keyword(s):  
Elderly Patient ◽  
Case Report ◽  
Sertoli Cell ◽  
Cell Tumor ◽  
Review Of Literature ◽  
Sertoli Cell Tumor ◽  
Patient Case

Testicular Tumors—Some New and a Few Perennial Problems

2008 ◽  
Vol 132 (4) ◽  
pp. 548-564 ◽  
Author(s):  
Robert H. Young
Keyword(s):  
Germ Cell ◽  
Sertoli Cell ◽  
Leydig Cell ◽  
Yolk Sac ◽  
Cell Tumor ◽  
Yolk Sac Tumor ◽  
Female Gonad ◽  
Sertoli Cell Tumor ◽  
Older Patient ◽  
Testicular Tumors

Abstract The histopathology of testicular tumors is presented, emphasizing new, unusual, or underemphasized aspects. Within the category of seminoma of the usual type, the recent literature has drawn attention to the presence in occasional tumors of solid or hollow tubules or spaces of varying sizes and shape that may result in cribriform or microcystic patterns, causing potential confusion with other neoplasms, most notably Sertoli cell tumor or yolk sac tumor. Although regions of typical neoplasia and awareness of this phenomenon usually will be diagnostic, immunohistochemistry may play a role in excluding Sertoli cell tumor or yolk sac tumor. Although immunohistochemistry can play an undoubted helpful role in this and selected other areas of testicular tumor evaluation, careful evaluation of the gross and routine microscopic features will solve the vast majority of diagnostic problems. An excellent review of immunohistochemistry in this area by R. E. Emerson, MD, and T. M. Ulbright, MD, is cited herein. Spermatocytic seminoma remains a crucial pitfall in diagnosis, and the pathologist must always be alert to the possible diagnosis when looking at a seminomatous neoplasm, particularly in an older patient, although about one third of these tumors occur in the usual seminoma age range. The embryonal carcinoma has a great diversity of patterns, which are briefly noted. The enigmatic and picturesque tumor, polyembryoma, which virtually never occurs in pure form but may be a confusing component of a variety of mixed germ cell tumors, is discussed and illustrated. The phenomenon of burnt-out germ cell neoplasia is also briefly noted and an excellent recent contribution is referred to. Within the sex cord–stromal family of neoplasms, recent contributions and elaborations of unusual morphologic features of Leydig cell tumors and Sertoli cell tumors are presented. Within the Leydig cell family, cyst formation, adipose metaplasia, calcification or ossification, and spindle cell patterns may be particularly confusing, and in the Sertoli cell family, a great array of patterns caused by differing admixtures of tubular, solid, and stromal components occur. The peculiar lesion, intratubular large cell hyalinizing Sertoli cell tumor, of young boys with Peutz-Jeghers syndrome, is briefly discussed. Some of the problems in the family of hematopoietic neoplasms are reviewed, these processes posing diverse problems in differential diagnosis and their correct recognition having crucial therapeutic implications. Although secondary tumors to the testis have not received the same attention in the literature as the similar phenomenon in the female gonad, remarkable examples of testicular spread of diverse neoplasms, usually carcinoma but rarely melanoma, are seen, and the pathologist should be alert to this possibility, particularly when examining an unusual morphology in an older patient. Finally, a few comments are made on the common paratesticular neoplasm, the adenomatoid tumor, highlighting its varied patterns and recent description of some of the issues that may arise when they undergo total or subtotal infarction.

Sign in / Sign up

Export Citation Format

Share Document