Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae

1985 ◽  
Vol 9 (4) ◽  
pp. 313-319 ◽  
Author(s):  
Elibieta Johannsen ◽  
Linda Eagle ◽  
Gail Bredenhann
1992 ◽  
Vol 38 (12) ◽  
pp. 1233-1237 ◽  
Author(s):  
Abindra S. Gupthar

A prototrophic strain of Saccharomyces cerevisiae CSIR Y190 MATa xyl−, resistant to high levels of ethanol, was hybridized with xylose-fermenting, auxotrophic mutants of Candida shehatae and Pichia stipitis through polyethylene glycol-induced protoplast fusion in an attempt to produce ethanol-tolerant, xylose-fermenting hybrids. Mononucleate fusants were obtained, but these dissociated into a mixture of parental-type segregants. Purified Candida- and Pichia-resembling segregants failed to acquire improved ethanol tolerance but expressed other novel properties of S. cerevisiae, suggesting that karyogamy was impaired after internuclear gene transfer. Key words: Pichia, Candida, Saccharomyces protoplast fusion.


2005 ◽  
Vol 7 (1-2) ◽  
pp. 263-280 ◽  
Author(s):  
Siu Wai Chiu ◽  
Vivien Wing Yan Luk ◽  
Stephen Yu ◽  
Peggy Lee ◽  
Natalie Wai ◽  
...  

2013 ◽  
Vol 32 (11) ◽  
pp. 1687-1701 ◽  
Author(s):  
Yan Yu ◽  
Wenxuan Ye ◽  
Li He ◽  
Xingkui Cai ◽  
Ting Liu ◽  
...  

1986 ◽  
Vol 861 ◽  
pp. 429-439 ◽  
Author(s):  
Kamnoon Kanchanapoom ◽  
Wendy F. Boss
Keyword(s):  

1997 ◽  
Vol 42 (3) ◽  
pp. 239-241 ◽  
Author(s):  
M. A. Loray ◽  
L. I. C. de Figueroa ◽  
M. Höfer

1992 ◽  
Vol 93 (3) ◽  
pp. 221-226 ◽  
Author(s):  
Soon Bai Chun ◽  
Jong Eon Chin ◽  
Suk Bai ◽  
Gil-Hwan An

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