Effects of the diuretics furosemide, ethacrynic acid, and chlorothiazide on gluconeogenesis from various substrates in rat kidney cortex slices

1972 ◽  
Vol 273 (1-2) ◽  
pp. 86-98 ◽  
Author(s):  
G. F�lgraff ◽  
H. N�nemann ◽  
D. Sudhoff
Keyword(s):  
Ethacrynic Acid ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Tamm—Horsfall Glycoprotein Release from Rat Kidney Cortex Slices in Vitro

1984 ◽  
Vol 67 (5) ◽  
pp. 529-534 ◽  
Author(s):  
P. K. Wirdnam ◽  
R. D. G. Milner
Keyword(s):  
Cell Membrane ◽  
Ethacrynic Acid ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
The Media ◽  
Cortex Slices ◽  
Kidney Cortex Slices ◽  
Krebs Henseleit Buffer

1. Rat kidney cortex slices were incubated for 30 min at 37°C in unmodified Krebs-Henseleit buffer containing aldosterone, vasopressin, theophylline, ethacrynic acid, frusemide, spironolactone or ouabain. 2. Tamm—Horsfall glycoprotein (THG) released into the media was measured by radioimmunoassay and at the end of each experiment the slices were homogenized and assayed for THG content. 3. Incubation of kidney cortex slices in unmodified buffer resulted in a significant increase in the slice THG content when compared with pre-incubation levels. The increase was prevented by puromycin or cycloheximide. 4. Incubation in ethacrynic acid (1 mmol/l) or frusemide (10 mmol/l) resulted in a significant increase in release of THG when compared with unmodified buffer. Puromycin or cycloheximide failed to prevent the increased release. 5. THG release induced by ethacrynic acid or frusemide is probably the result of an aggregation-disaggregation reaction on the cell membrane. It is suggested that the action of the chloride inhibiting diuretics, ethacrynic acid and frusemide, is mediated in some way via THG.

Effect of Pentobarbital Sodium on Uptake of PAH by Rat Kidney Cortex Slices in Vitro

1958 ◽  
Vol 195 (2) ◽  
pp. 343-346 ◽  
Author(s):  
E. J. Støren
Keyword(s):  
Oxygen Consumption ◽  
Renal Cortex ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Tissue Respiration ◽  
Rat Kidney Cortex ◽  
Pentobarbital Sodium ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Active uptake of PAH by rat renal cortex slices was studied by the method of Cross and Taggart. Uptake was determined at low and at high medium concentrations of PAH. Pentobarbital sodium in concentrations comparable to those found in plasma during anesthesia, significantly depressed the uptake of PAH on all occasions. Simultaneously oxygen consumption was reduced. Acetate failed to stimulate PAH uptake in the presence of pentobarbital, although tissue respiration was restored to normal.

Effect of Hippurate on Glucose Utilization in Rat Kidney Cortex Slices

1991 ◽  
Vol 14 (1-2) ◽  
pp. 42-47 ◽  
Author(s):  
Viera Spustová ◽  
Rastislav Dzúrik
Keyword(s):  
Glucose Utilization ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Renal handling of [(14)C]mannose

1976 ◽  
Vol 231 (1) ◽  
pp. 9-13 ◽  
Author(s):  
PD McNamara ◽  
C Rea ◽  
B Ozegovic ◽  
S Segal
Keyword(s):  
Rat Kidney ◽  
Neutral Fraction ◽  
Kidney Cortex ◽  
Renal Handling ◽  
Rat Kidney Cortex ◽  
Intracellular Fluid ◽  
Adult Rat ◽  
Extensive Metabolism ◽  
Cortex Slices ◽  
Kidney Cortex Slices

The uptake of D-[(14)C]mannose by adult rat kidney cortex slices has been examined. The process involved appeared to be saturable and oxygen dependent and to be inhibited by glucose, galactose, chi-methyl-D-glucoside, and phlorizin. Extensive metabolism of the sugar occurred. (14)CO(2) was produced to an extent comparable to that from D-glucose. Radioactive compounds accumulated in the tissue, approximately 50% of which were in the form of phosphorylated or other polar intermediates. The remaining neutral fraction consisted of mannose with a small amount of label in glucose and an unidentified compound which behaves like an alditol. Mannose concentration in the intracellular fluid was never greater than that of the medium.

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