rat kidney cortex
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2015 ◽  
Vol 1 (6) ◽  
pp. 311-321
Author(s):  
Safia Mohamed Hassan ◽  
Eman Ahmed Youssef


Author(s):  
R. C. Scaduto ◽  
T. Strzelecki ◽  
G. C. Keyser ◽  
W. J. Hoover ◽  
A. C. Schoolwerth


2015 ◽  
Vol 93 (4) ◽  
pp. 299-308 ◽  
Author(s):  
Elizabeth Alejandrina Guzmán-Hernández ◽  
Rafael Villalobos-Molina ◽  
María Alicia Sánchez-Mendoza ◽  
Leonardo Del Valle-Mondragón ◽  
Gustavo Pastelín-Hernández ◽  
...  

We investigated the involvement of cyclooxygenase-2 (COX-2) and the renin–angiotensin system in NG-nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Male Wistar rats were treated with l-NAME (75.0 mg·(kg body mass)−1·day−1, in their drinking water) for different durations (1–33 days). COX-2 and renin mRNA were measured using real-time PCR in the renal cortex, and prostanoids were assessed in the renal perfusate, whereas angiotensin II (Ang II) and Ang (1-7) were quantified in plasma. In some rats, nitric oxide synthase inhibition was carried out in conjunction with oral administration of captopril (30.0 mg·kg−1·day−1) or celecoxib (1.0 mg·kg−1·day−1) for 2 or 19 days. We found a parallel increase in renocortical COX-2 and renin mRNA starting at day 2 of treatment with l-NAME, and both peaked at 19–25 days. In addition, l-NAME increased renal 6-Keto-PGF1α (prostacyclin (PGI2) metabolite) and plasma Ang II from day 2, but reduced plasma Ang (1-7) at day 19. Captopril prevented the increase in blood pressure, which was associated with lower plasma Ang II and increased COX-2-derived 6-Keto-PGF1α at day 2 and plasma Ang (1-7) at day 19. Celecoxib partially prevented the increase in blood pressure; this effect was associated with a reduction in plasma Ang II. These findings indicate that renal COX-2 expression increased in parallel with renin expression, renal PGI2 synthesis, and plasma Ang II in l-NAME-induced hypertension.



2014 ◽  
Vol 307 (11) ◽  
pp. F1249-F1262 ◽  
Author(s):  
Jens Christian Brasen ◽  
James L. Burford ◽  
Alicia A. McDonough ◽  
Niels-Henrik Holstein-Rathlou ◽  
Janos Peti-Peterdi

The proximal tubule Na+/H+ exchanger 3 (NHE3), located in the apical dense microvilli (brush border), plays a major role in the reabsorption of NaCl and water in the renal proximal tubule. In response to a rise in blood pressure NHE3 redistributes in the plane of the plasma membrane to the base of the brush border, where NHE3 activity is reduced. This NHE3 redistribution is assumed to provoke pressure natriuresis; however, it is unclear how NHE3 redistribution per se reduces NHE3 activity. To investigate if the distribution of NHE3 in the brush border can change the reabsorption rate, we constructed a spatiotemporal mathematical model of NHE3-mediated Na+ reabsorption across a proximal tubule cell and compared the model results with in vivo experiments in rats. The model predicts that when NHE3 is localized exclusively at the base of the brush border, it creates local pH microdomains that reduce NHE3 activity by >30%. We tested the model's prediction experimentally: the rat kidney cortex was loaded with the pH-sensitive fluorescent dye BCECF, and cells of the proximal tubule were imaged in vivo using confocal fluorescence microscopy before and after an increase of blood pressure by ∼50 mmHg. The experimental results supported the model by demonstrating that a rise of blood pressure induces the development of pH microdomains near the bottom of the brush border. These local changes in pH reduce NHE3 activity, which may explain the pressure natriuresis response to NHE3 redistribution.



2013 ◽  
Vol 305 (5) ◽  
pp. F628-F640 ◽  
Author(s):  
Kevin L. Schauer ◽  
Dana M. Freund ◽  
Jessica E. Prenni ◽  
Norman P. Curthoys

Metabolic acidosis is a relatively common pathological condition that is defined as a decrease in blood pH and bicarbonate concentration. The renal proximal convoluted tubule responds to this condition by increasing the extraction of plasma glutamine and activating ammoniagenesis and gluconeogenesis. The combined processes increase the excretion of acid and produce bicarbonate ions that are added to the blood to partially restore acid-base homeostasis. Only a few cytosolic proteins, such as phosphoenolpyruvate carboxykinase, have been determined to play a role in the renal response to metabolic acidosis. Therefore, further analysis was performed to better characterize the response of the cytosolic proteome. Proximal convoluted tubule cells were isolated from rat kidney cortex at various times after onset of acidosis and fractionated to separate the soluble cytosolic proteins from the remainder of the cellular components. The cytosolic proteins were analyzed using two-dimensional liquid chromatography and tandem mass spectrometry (MS/MS). Spectral counting along with average MS/MS total ion current were used to quantify temporal changes in relative protein abundance. In all, 461 proteins were confidently identified, of which 24 exhibited statistically significant changes in abundance. To validate these techniques, several of the observed abundance changes were confirmed by Western blotting. Data from the cytosolic fractions were then combined with previous proteomic data, and pathway analyses were performed to identify the primary pathways that are activated or inhibited in the proximal convoluted tubule during the onset of metabolic acidosis.



2010 ◽  
Vol 299 (2) ◽  
pp. C374-C380 ◽  
Author(s):  
Joseph Satriano ◽  
Hadi Mansoury ◽  
Aihua Deng ◽  
Kumar Sharma ◽  
Volker Vallon ◽  
...  

Diabetic nephropathy is the commonest cause of end-stage renal disease. Inordinate kidney growth and glomerular hyperfiltration at the very early stages of diabetes are putative antecedents to this disease. The kidney is the only organ that grows larger with the onset of diabetes mellitus, yet there remains confusion about the mechanism and significance of this growth. Here we show that kidney proximal tubule cells in culture transition to senescence in response to oxidative stress. We further determine the temporal expression of G1 phase cell cycle components in rat kidney cortex at days 4 and 10 of streptozotocin diabetes to evaluate changes in this growth response. In diabetic rats we observe increases in kidney weight-to-body weight ratios correlating with increases in expression of the growth-related proteins in the kidney at day 4 after induction of diabetes. However, at day 10 we find a decrease in this profile in diabetic animals coincident with increased cyclin-dependent kinase inhibitor expressions. We observe no change in caspase-3 expression in the diabetic kidneys at these early time points; however, diabetic animals demonstrate reduced kidney connexin 43 and increased plasminogen activator inhibitor-1 expressions and increased senescence-associated β-galactosidase activity in cortical tubules. In summary, diabetic kidneys exhibit an early temporal induction of growth phase components followed by their suppression concurrent with the induction of cyclin-dependent kinase inhibitors and markers of senescence. These data delineate a phenotypic change in cortical tubules early in the pathogenesis of diabetes that may contribute to further downstream complications of the disease.



Biochimie ◽  
2010 ◽  
Vol 92 (2) ◽  
pp. 128-135 ◽  
Author(s):  
V. Ventrella ◽  
J.R. Elvir ◽  
A.R. Borgatti ◽  
G. Trigari ◽  
T. Proverbio ◽  
...  


2009 ◽  
Vol 137 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Lin Wang ◽  
Jianji Li ◽  
Jingui Li ◽  
Zongping Liu


2009 ◽  
Vol 239 (3) ◽  
pp. 284-296 ◽  
Author(s):  
Vilim Žlender ◽  
Davorka Breljak ◽  
Marija Ljubojević ◽  
Dubravka Flajs ◽  
Daniela Balen ◽  
...  


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