Differential staining of human chromosomes with quinacrine

1973 ◽  
Vol 76 (3) ◽  
pp. 1119-1122
Author(s):  
V. A. Benyush
Keyword(s):  
Differential Staining ◽  
Human Chromosomes

scholarly journals Differential Staining of Human Chromosomes by Treatment with Urea

1971 ◽  
Vol 47 (9) ◽  
pp. 729-731 ◽  
Author(s):  
Yukimasa SHIRAISHI ◽  
Tosihide H. YOSIDA
Keyword(s):  
Differential Staining ◽  
Human Chromosomes

INFLUENCE OF CYSTEAMINE ON DIFFERENTIAL STAINING OF BUdR-SUBSTITUTED HUMAN CHROMOSOMES

1979 ◽  
Vol 21 (1) ◽  
pp. 145-149 ◽  
Author(s):  
W. Scheid
Keyword(s):  
Visible Light ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Strand Breaks ◽  
Light System

In 5-bromodeoxyuridine (BUdR)-substituted human chromosomes stained with 4′-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

Ag-Nor staining in human chromosomes: Differential staining in normal and leukemic bone-marrow samples

1985 ◽  
Vol 36 (6) ◽  
pp. 647-649 ◽  
Author(s):  
K. C. Arden ◽  
S. Pathak ◽  
L. S. Frankel ◽  
A. Zander
Keyword(s):  
Bone Marrow ◽  
Differential Staining ◽  
Human Chromosomes

An Analysis of Factors for the Differential Staining of Sister Chromatids in Human Chromosomes Using Giemsa

1978 ◽  
Vol 27 ◽  
pp. 81-87
Author(s):  
Reita Nand ◽  
P. K. Ghosh
Keyword(s):  
Conformational Changes ◽  
Proteolytic Enzymes ◽  
Staining Technique ◽  
Differential Staining ◽  
Chromosomal Protein ◽  
Human Chromosomes ◽  
Trypsin Treatment ◽  
Slide Preparation ◽  
Sister Chromatids

Various reagents were tested for the purpose of developing an improved Giemsa staining technique for the differential staining of sister chromatids in human chromosomes. Reagents like acids, bases, buffers, protein denaturants and proteolytic enzymes were all potent inducers of differential staining. The best results were obtained by brief trypsinization followed by extraction of nucleic acids by incubation in hot HCl. There was poor contrast between unifilarly and bifilarly BrdU substituted chromatids in slides from which trypsin treatment was omitted. The method of slide preparation as they affect the spreads of BrdU substituted metaphases were also evaluated. The results support the role of these reagents in the conformational changes and structural lesions of chromosomal protein leading to differential staining.

A Study of the Ultrastructure of Visual Cell Outer Segment Membranes, Using a Water-Miscible Embedding Medium and Differential Staining

1972 ◽  
Vol 30 ◽  
pp. 50-51
Author(s):  
Anthony J. Godfrey
Keyword(s):  
Outer Segment ◽  
Protein Denaturation ◽  
Uranyl Acetate ◽  
Differential Staining ◽  
Visual Cell ◽  
Lead Citrate ◽  
Chick Retina ◽  
Dark Staining ◽  
Embedding Medium ◽  
The Individual

Aldehyde-fixed chick retina was embedded in a water-containing resin of glutaraldehyde and urea, without dehydration. The loss of lipids and other soluble tissue components, which is severe in routine methods involving dehydration, was thereby minimized. Osmium tetroxide post-fixation was not used, lessening the amount of protein denaturation which occurred. Ultrathin sections were stained with 1, uranyl acetate and lead citrate, 2, silicotungstic acid, or 3, osmium vapor, prior to electron microscope examination of visual cell outer segment ultrastructure, at magnifications up to 800,000.Sections stained with uranyl acetate and lead citrate (Fig. 1) showed that the individual disc membranes consisted of a central lipid core about 78Å thick in which dark-staining 40Å masses appeared to be embedded from either side.

Automatic Karyotyping of Human Chromosomes Using Band Patterns

2012 ◽  
Vol 2 (12) ◽  
pp. 154-156 ◽  
Author(s):  
Priya Chaku ◽  
◽  
Pooja Shah
Keyword(s):  
Human Chromosomes
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