Effect of 1,2?5,6-dianhydrogalactitol and 1,2?5,6-dianhydro-3,4-diacetyldianhydrogalactitol on DNA synthesis by cells of mouse melanoma B16, bone marrow, gastrointestinal mucosa, spleen, and liver

1984 ◽  
Vol 98 (3) ◽  
pp. 1203-1205
Author(s):  
I. S. Sokolova ◽  
I. Elekes ◽  
L. �tv�s ◽  
L. B. Gorbacheva
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Gastrointestinal Mucosa ◽  
Mouse Melanoma ◽  
Melanoma B16

Thrombopoietin-Induced Stimulation of Megakaryocyte- Enriched Bone Marrow Cultures

1979 ◽  
Author(s):  
K. L. Kellar ◽  
B. L. Evatt ◽  
C. R. McGrath ◽  
R. B. Ramsey
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Rabbit Plasma ◽  
Bone Marrow Cultures ◽  
Plasma Fractions ◽  
Marrow Cultures ◽  
Stimulation Of

Liquid cultures of bone marrow cells enriched for megakaryocytes were assayed for incorporation of 3H-thymidine (3H-TdR) into acid-precipitable cell digests to determine the effect of thrombopoietin on DNA synthesis. As previously described, thrombopoietin was prepared by ammonium sulfate fractionation of pooled plasma obtained from thrombocytopenic rabbits. A control fraction was prepared from normal rabbit plasma. The thrombopoietic activity of these fractions was determined in vivo with normal rabbits as assay animals and the rate of incorporation of 75Se-selenomethionine into newly formed platelets as an index of thrombopoietic activity of the infused material. Guinea pig megakaryocytes were purified using bovine serum albumin gradients. Bone marrow cultures containing 1.5-3.0x104 cells and 31%-71% megakaryocytes were incubated 18 h in modified Dulbecco’s MEM containing 10% of the concentrated plasma fractions from either thrombocytopenic or normal rabbits. In other control cultures, 0.9% NaCl was substituted for the plasma fractions. 3H-TdR incorporation was measured after cells were incubated for 3 h with 1 μCi/ml. The protein fraction containing thrombopoietin-stimulating activity caused a 25%-31% increase in 3H-TdR incorporation over that in cultures which were incubated with the similar fraction from normal plasma and a 29% increase over the activity in control cultures to which 0.9% NaCl had been added. These data suggest that thrombopoietin stimulates DNA synthesis in megakaryocytes and that this tecnique may be useful in assaying thrombopoietin in vitro.

GM3 Signals Regulating TNF-Alpha Expression Are Mediated by Rictor and Arhgdib in Mouse Melanoma B16 Cells

Oncology ◽  
2007 ◽  
Vol 73 (5-6) ◽  
pp. 430-438 ◽  
Author(s):  
Pu Wang ◽  
Xiaoyan Yang ◽  
Peixing Wu ◽  
Jinghai Zhang ◽  
Toshinori Sato ◽  
...  
Keyword(s):  
Tnf Alpha ◽  
Mouse Melanoma ◽  
Melanoma B16

Megaloblastic anaemia and miscellaneous deficiency anaemias

2010 ◽  
pp. 4402-4420 ◽  
Author(s):  
A.V. Hoffbrand ◽  
Drew Provan
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Dna Synthesis ◽  
Red Blood Cell ◽  
Megaloblastic Anaemia ◽  
Vitamin B ◽  
Inhibition Of Dna Synthesis

Megaloblastic anaemias are characterized by red blood cell macrocytosis. They arise because of inhibition of DNA synthesis in the bone marrow, usually due to deficiency of one or other of vitamin B12 (cobalamin) or folate, but sometimes as a consequence of a drug or a congenital or acquired biochemical defect that disturbs their metabolism, or affects DNA synthesis independent of vitamin B...

scholarly journals Embryonic Stem Cell–Derived Dendritic Cells Expressing Glypican-3, a Recently Identified Oncofetal Antigen, Induce Protective Immunity against Highly Metastatic Mouse Melanoma, B16-F10

2006 ◽  
Vol 66 (4) ◽  
pp. 2414-2422 ◽  
Author(s):  
Yutaka Motomura ◽  
Satoru Senju ◽  
Tetsuya Nakatsura ◽  
Hidetake Matsuyoshi ◽  
Shinya Hirata ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Protective Immunity ◽  
Embryonic Stem ◽  
Mouse Melanoma ◽  
Melanoma B16

Radiation Effects on DNA Synthesis and Cell Division in the Bone-marrow of the Mouse

1966 ◽  
Vol 11 (5) ◽  
pp. 435-443 ◽  
Author(s):  
E. Frindel ◽  
F. Charruyer ◽  
M. Tubiana ◽  
H.S. Kaplan ◽  
E.L. Alpen
Keyword(s):  
Bone Marrow ◽  
Cell Division ◽  
Dna Synthesis ◽  
Radiation Effects
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