Load-piston pressure stabilizer and its field of application

1973 ◽  
Vol 16 (5) ◽  
pp. 703-705
Author(s):  
A. M. Kipnis
Keyword(s):  
2004 ◽  
Vol 1 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Song Ai-Huan ◽  
Li Hong-Ye ◽  
Liu Xiao-Hong

AbstractThe effects of some factors on the isolation of protoplasts fromPenicillium digitatumwere studied, including the appropriate material (young mycelia and generating spores), the concentrations of enzyme and osmotic pressure stabilizers, reaction time and reaction temperature. Results demonstrated that germinating spores were an ideal material resource for the isolation ofP. digitatumprotoplasts. Highest yield and quality ofP. digitatumprotoplasts were obtained by shaking germinating spores suspended in a solution of 10 mg/ml Lywallzyme™ dissolved in 0.7 M NaCl as osmotic pressure stabilizer at 80 rev/min and 30°C for 3.0–3.5 h. The regeneration rate of the isolated protoplasts was as high as 24.9% on double-layer Czapek medium containing 0.7 M NaCl. Additionally, observation of the protoplast release pattern showed that the protoplasts ofP. digitatumwere released primarily from the hyphal apex and occasionally from the subapical or original sites of germinating tubes. The protoplasts ofP. digitatumwere regenerated in a direct manner of either yeast-like cell development or mycelium formation.


Endoscopy ◽  
1972 ◽  
Vol 4 (01) ◽  
pp. 15-19
Author(s):  
K. Yamada ◽  
Sh. Suzuki ◽  
T. Kozu ◽  
M. Endo ◽  
T. Takemoto

2011 ◽  
Vol 183-185 ◽  
pp. 1522-1526
Author(s):  
Hui Min Yue ◽  
Qian Yang

The factors of species of the medium, spawn age, species of the enzyme, time of hydrolysis, hydrolysis temperature, osmotic pressure stabilizer and regeneration medium, which have important effects on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast, were discussed in this paper. These results showed that the hydrolysis condition can be described as following: Chaetomium cupreum mycelium with spawn age about 48h, the concentration of the lytic enzymes about 2% and the time of enzymatic hydrolysis 2h. The regeneration condition of the protoplast is that: Chaetomium cupreum mycelium with the spawn age 6h, the concentration of the lytic enzymes 2%, the temperature of 25°C and the time of enzymatic hydrolysis 1.5h. We can get the Chaetomium cupreum protoplast, regenerating in the RA Medium with the sorbitol, which regeneration can be over 50%.


1966 ◽  
Vol 4 (1) ◽  
pp. 56-58
Author(s):  
S. Kh. Sheremet'ev

2005 ◽  
Vol 24 (2) ◽  
Author(s):  
M.S. Kulyk ◽  
К.I. Kapitanchuk ◽  
P.I. Grekov ◽  
S.P. Onischenko

1960 ◽  
Vol 199 (2) ◽  
pp. 319-324 ◽  
Author(s):  
B. G. Zimmerman ◽  
M. J. Brody ◽  
L. Beck

The action of ganglionic blocking agents on the cardiac output has been studied in intact, closed-chested animals whose cardiac filling pressure was maintained constant by means of a pressure-stabilizer operating through a jugular catheter introduced into the right heart. Under these conditions cardiodynamic consequences of venous pooling are negated. Hexamethonium produces a fall in cardiac output and blood pressure comparable to that observed in animals without controlled venous pressure. In cardiac sympathectomized animals the cardiac output increases slightly following the administration of hexamethonium. These observations indicate that the ganglion blocking agents reduce cardiac output primarily by blocking tonic sympathetic activity to the heart. The decrease in cardiac output is brought about by a reduction in both stroke volume and heart rate. Sympathetic tone to the blood vessels is reduced by a low dose of hexamethonium before a significant depression in cardiac output occurs.


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