Dose- and time-dependent hippocampal cholinergic lesions induced by ethylcholine mustard aziridinium ion: Effects of nerve growth factor, GM1 ganglioside, and vitamin E

1988 ◽  
Vol 13 (8) ◽  
pp. 685-692 ◽  
Author(s):  
Gail V. W. Johnson ◽  
Michele Simonato ◽  
Richard S. Jope

1988 ◽  
Vol 448 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Guido Vantini ◽  
Mariella Fusco ◽  
Emilio Bigon ◽  
Alberta Leon


2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Naoki Wada ◽  
Takahiro Shimizu ◽  
Nobutaka Shimizu ◽  
Pradeep Tyagi ◽  
William de Groat ◽  
...  




2006 ◽  
Vol 318 (2) ◽  
pp. 709-724 ◽  
Author(s):  
Alvin V. Terry ◽  
Vinay Parikh ◽  
Debra A. Gearhart ◽  
Anilkumar Pillai ◽  
Elizabeth Hohnadel ◽  
...  




Endocrinology ◽  
2008 ◽  
Vol 149 (4) ◽  
pp. 1678-1686 ◽  
Author(s):  
Christoph Schell ◽  
Martin Albrecht ◽  
Christine Mayer ◽  
J. Ullrich Schwarzer ◽  
Monica B. Frungieri ◽  
...  

Testicular peritubular cells are myofibroblastic cells, which represent the major cellular components of the wall of the seminiferous tubules. In men their phenotypic characteristics, including possible secretory activity and regulation, are not well known, in neither normal nor pathologically altered testes. Especially in testes of men with impaired spermatogenesis, the cytoarchitecture of the tubular wall is frequently remodeled and presents fibrotic thickening, increased innervation, and infiltration by macrophages and mast cells. The latter are two sources of TNF-α. The purpose of our study was to explore human testicular peritubular cells and mechanisms of their regulation. To this end we primarily studied cultured human testicular peritubular cells (HTPCs), isolated from adult human testes. Having established that HTPCs express TNF-α receptors 1 and 2 and respond to recombinant human TNF-α by a rapid phosphorylation of ERK1/2, we used complementary approaches, including gene array/RT-PCR studies, Western blotting/immunocytochemistry, and ELISA techniques to study phenotypic characteristics of HTPCs and actions of TNFα. We found that HTPCs express the nerve growth factor gene and TNF-α-stimulated mRNA levels and secretion of nerve growth factor in a dose- and time-dependent manner. Similarly, monocyte chemoattractant protein-1 was identified as a product of HTPCs, which was regulated by TNF-α in a concentration- and time-dependent manner. TNF-α furthermore strongly enhanced expression and/or synthesis of other inflammatory molecules, namely IL-6 and cyclooxygenase-2. Active cyclooxygenase-2 is indicated by increased prostaglandin D2 levels. In addition, intercellular adhesion molecule-1, which was not detected at protein level in the absence of TNF-α, was induced upon TNF-α stimulation. In conclusion, these results provide novel insights into the nature of human peritubular cells, which are able to secrete potent signaling molecules and are regulated by TNF-α. These results also hint to an as-yet-unknown role of peritubular cells in normal human testis and involvement in the pathomechanisms associated with impaired spermatogenesis in men.







2011 ◽  
Vol 27 (3) ◽  
pp. 209-218 ◽  
Author(s):  
Arianna Calza ◽  
Fulvio Florenzano ◽  
Daniela Pellegrini ◽  
Paola Tirassa


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