Idiopathic amyloidosis in the stone marten (Martes foina): Identification of amyloid fibril proteins in tissue sections using the immunoperoxidase technique

1980 ◽  
Vol 41 (6) ◽  
pp. 465-468 ◽  
Author(s):  
R. P. Linke ◽  
O. Geisel ◽  
M. Eulitz ◽  
W. B. J. Nathrath
1984 ◽  
Vol 32 (3) ◽  
pp. 322-328 ◽  
Author(s):  
R P Linke

Monoclonal antibodies against amyloid fibril protein AA were produced by cell fusion of murine P3 X 63-Ag8.653 myeloma cells with spleen cells of immunized Balb/c mice. To increase immunogenicity, protein AA was coupled to horseradish peroxidase (HRP) or human high molecular weight kininogen (HMWK). Using micro-ELISA (enzyme-linked immunosorbent essay) seven hybridoma cell lines secreting antibodies that specifically bind to protein AA have been selected and cloned. When applied to formalin-fixed paraffin sections of a variety of different amyloid types using immunoperoxidase methods, five monoclonal antibodies bound specifically and strongly to amyloid only of the AA type. Since a series of different AA-amyloids could be stained, these reagents may be used to routinely diagnose AA-amyloidosis in tissue sections. A monoclonal antibody against HRP has also been produced that has been utilized to develop a monoclonal peroxidase-antiperoxidases (PAP) complex. When three immunoperoxidase methods were compared, the sensitivity of a conventional rat PAP was comparable to the monoclonal PAP complex, but the latter was easier to handle. Both methods were more sensitive than the indirect immunoperoxidase technique.


2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Kyriaki Kitikidou ◽  
Malamati Papakosta ◽  
Dimitris Bakaloudis ◽  
Christos Vlachos

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 539
Author(s):  
Urška Kuhar ◽  
Diana Žele Vengušt ◽  
Urška Jamnikar-Ciglenečki ◽  
Gorazd Vengušt

Papillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosal infections in humans and various domestic and wild animals. Nevertheless, there is limited knowledge about PVs in wildlife hosts, including mustelid species. This study describes a case in stone marten (Martes foina) with a clinical manifestation of skin tumor, which is rather atypical for infections with PVs. The result of the papillomavirus PCR performed on the skin tumor sample was positive, and the complete PV genome was determined in the studied sample using next-generation sequencing technology. The analysis of the PV genome revealed infection of the stone marten with a putative new PV type belonging to the Dyonupapillomavirus genus. The proposed new stone marten PV type was named MfoiPV1.


2018 ◽  
Vol 16 ◽  
pp. 163-172
Author(s):  
C. Olmedo ◽  
◽  
P. Refoyo ◽  
D. García ◽  
B. Muñoz ◽  
...  

Mammal Study ◽  
2013 ◽  
Vol 38 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Masumi Hisano ◽  
Evgeniy G. Raichev ◽  
Hiroshi Tsunoda ◽  
Ryuichi Masuda ◽  
Yayoi Kaneko

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Dimitrios E. Bakaloudis ◽  
Christos G. Vlachos ◽  
Malamati A. Papakosta ◽  
Vasileios A. Bontzorlos ◽  
Evangelos N. Chatzinikos

Stone martens (Martes foina) are documented as generalist throughout their distributional range whose diet composition is affected by food availability. We tested if this occurs and what feeding strategies it follows in a typical Mediterranean ecosystem in Central Greece by analysing contents from 106 stomachs, seasonally collected from three different habitats during 2003–2006. Seasonal variation in diet and feeding strategies was evident and linked to seasonal nutritional requirements, but possibly imposed by strong interference competition and intraguild predation. Fleshy fruits and arthropods predominated in the diet, but also mammals and birds were frequently consumed. An overall low dietary niche breadth (BA=0.128) indicated a fruit specialization tendency. A generalised diet occurred in spring with high individual specialisation, whereas more animal-type prey was consumed than fruits. A population specialization towards fruits was indicated during summer and autumn, whereas insects were consumed occasionally by males. In those seasons it switched to more clumped food types such as fruits and insects. In winter it selectively exploited both adult and larvae insects and partially fruits overwinter on plants. The tendency to consume particular prey items seasonally reflected both the population specialist behaviour and the individual flexibility preyed on different food resources.


2004 ◽  
Vol 51 (2) ◽  
pp. 49-54 ◽  
Author(s):  
T. Muller ◽  
J. Cox ◽  
W. Peter ◽  
R. Schafer ◽  
N. Johnson ◽  
...  
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